Burkholderia cenocepacia lectin A binding to heptoses from the
bacterial lipopolysaccharide

J 2012

Burkholderia cenocepacia lectin A binding to heptoses from the bacterial lipopolysaccharide

MARCHETTI, Roberta, Lenka MALINOVSKÁ, Emilie LAMEIGNERE, Lenka ADAMOVÁ, Cristina DE CASTRO et. al.

Basic information

Original name

Burkholderia cenocepacia lectin A binding to heptoses from the bacterial lipopolysaccharide

Authors

MARCHETTI, Roberta (380 Italy), Lenka MALINOVSKÁ (203 Czech Republic, belonging to the institution), Emilie LAMEIGNERE (250 France), Lenka ADAMOVÁ (203 Czech Republic, belonging to the institution), Cristina DE CASTRO (380 Italy), Gianluca CIOCI (250 France), Christian STANETTY (40 Austria), Paul KOSMA (40 Austria), Antonio MOLINARO (380 Italy), Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution), Anne IMBERTY (250 France) and Alba SILIPO (380 Italy)

Edition

Glycobiology, Oxford, Oxford University Press, 2012, 0959-6658

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.537

RIV identification code

RIV/00216224:14740/12:00057548

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/glycob/cws105

UT WoS

000308011800011

Keywords in English

bacterial lectin;heptose;lipopolysaccharide;NMR;titration microcalorimetry

Abstract

V originále

Bacteria from the Burkholderia cepacia complex (Bcc) cause highly contagious pneumonia among cystic fibrosis (CF) patients. Among them, Burkholderia cenocepacia is one of the most dangerous in the Bcc and is the most frequent cause of morbidity and mortality in CF patients. Indeed, it is responsible of “cepacia syndrome”, a deadly exacerbation of infection, that is the main cause of poor outcomes in lung transplantation. Burkholderia cenocepacia produces several soluble lectins with specificity for fucosylated and mannosylated glycoconjugates. These lectins are present on the bacterial cell surface and it has been proposed that they bind to lipopolysaccharide epitopes. In this work, we report on the interaction of one B. cenocepacia lectin, BC2L-A, with heptose and other manno configured sugar residues. Saturation transfer difference NMR spectroscopy studies of BC2L-A with different mono- and disaccharides demonstrated the requirement of manno configuration with the hydroxyl or glycol group at C6 for the binding process. The crystal structure of BC2L-A complexed with the methyl-heptoside confirmed the location of the carbohydrate ring in the binding site and elucidated the orientation of the glycol tail, in agreement with NMR data. Titration calorimetry performed on monosaccharides, heptose disaccharides and bacterial heptose-containing oligosaccharides and polysaccharides confirmed that bacterial cell wall contains carbohydrate epitopes that can bind to BC2L-A. Additionally, the specific binding of fluorescent BC2L-A lectin on B. cenocepacia bacterial surface was demonstrated by microscopy.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GA303/09/1168, research and development project

Name: Lektiny z lidských patogenů - struktura, funkce, inženýrství

Investor: Czech Science Foundation, Lectins from human pathogens - structure, function, engineering

GD301/09/H004, research and development project

Name: Molekulární a strukturní biologie vybraných cytostatik. Od mechanistických studií k chemoterapii rakoviny

Investor: Czech Science Foundation

Files attached

2012_glycobiology.pdf

Request the author's version of the file

Citovat

MARCHETTI, Roberta, Lenka MALINOVSKÁ, Emilie LAMEIGNERE, Lenka ADAMOVÁ, Cristina DE CASTRO, Gianluca CIOCI, Christian STANETTY, Paul KOSMA, Antonio MOLINARO, Michaela WIMMEROVÁ, Anne IMBERTY and Alba SILIPO. Burkholderia cenocepacia lectin A binding to heptoses from the bacterial lipopolysaccharide. Glycobiology. Oxford: Oxford University Press, 2012, vol. 22, No 10, p. 1387-1398. ISSN 0959-6658. Available from: https://dx.doi.org/10.1093/glycob/cws105.

@article{990466,
   author = {Marchetti, Roberta and Malinovská, Lenka and Lameignere, Emilie and Adamová, Lenka and de Castro, Cristina and Cioci, Gianluca and Stanetty, Christian and Kosma, Paul and Molinaro, Antonio and Wimmerová, Michaela and Imberty, Anne and Silipo, Alba},
   article_location = {Oxford},
   article_number = {10},
   doi = {http://dx.doi.org/10.1093/glycob/cws105},
   keywords = {bacterial lectin;heptose;lipopolysaccharide;NMR;titration microcalorimetry},
   language = {eng},
   issn = {0959-6658},
   journal = {Glycobiology},
   title = {Burkholderia cenocepacia lectin A binding to heptoses from the bacterial lipopolysaccharide},
   url = {http://glycob.oxfordjournals.org/content/22/10/1387.abstract},
   volume = {22},
   year = {2012}
}

TY  - JOUR
ID  - 990466
AU  - Marchetti, Roberta - Malinovská, Lenka - Lameignere, Emilie - Adamová, Lenka - de Castro, Cristina - Cioci, Gianluca - Stanetty, Christian - Kosma, Paul - Molinaro, Antonio - Wimmerová, Michaela - Imberty, Anne - Silipo, Alba
PY  - 2012
TI  - Burkholderia cenocepacia lectin A binding to heptoses from the bacterial lipopolysaccharide
JF  - Glycobiology
VL  - 22
IS  - 10
SP  - 1387-1398
EP  - 1387-1398
PB  - Oxford University Press
SN  - 09596658
KW  - bacterial lectin;heptose;lipopolysaccharide;NMR;titration microcalorimetry
UR  - http://glycob.oxfordjournals.org/content/22/10/1387.abstract
L2  - http://glycob.oxfordjournals.org/content/22/10/1387.abstract
N2  - Bacteria from the Burkholderia cepacia complex (Bcc) cause highly contagious pneumonia among cystic fibrosis (CF) patients. Among them, Burkholderia cenocepacia is one of the most dangerous in the Bcc and is the most frequent cause of morbidity and mortality in CF patients. Indeed, it is responsible of “cepacia syndrome”, a deadly exacerbation of infection, that is the main cause of poor outcomes in lung transplantation. Burkholderia cenocepacia produces several soluble lectins with specificity for fucosylated and mannosylated glycoconjugates. These lectins are present on the bacterial cell surface and it has been proposed that they bind to lipopolysaccharide epitopes. In this work, we report on the interaction of one B. cenocepacia lectin, BC2L-A, with heptose and other manno configured sugar residues. Saturation transfer difference NMR spectroscopy studies of BC2L-A with different mono- and disaccharides demonstrated the requirement of manno configuration with the hydroxyl or glycol group at C6 for the binding process. The crystal structure of BC2L-A complexed with the methyl-heptoside confirmed the location of the carbohydrate ring in the binding site and elucidated the orientation of the glycol tail, in agreement with NMR data. Titration calorimetry performed on monosaccharides, heptose disaccharides and bacterial heptose-containing oligosaccharides and polysaccharides confirmed that bacterial cell wall contains carbohydrate epitopes that can bind to BC2L-A. Additionally, the specific binding of fluorescent BC2L-A lectin on B. cenocepacia bacterial surface was demonstrated by microscopy.
ER  -

MARCHETTI, Roberta, Lenka MALINOVSKÁ, Emilie LAMEIGNERE, Lenka ADAMOVÁ, Cristina DE CASTRO, Gianluca CIOCI, Christian STANETTY, Paul KOSMA, Antonio MOLINARO, Michaela WIMMEROVÁ, Anne IMBERTY and Alba SILIPO. Burkholderia cenocepacia lectin A binding to heptoses from the bacterial lipopolysaccharide. \textit{Glycobiology}. Oxford: Oxford University Press, 2012, vol.~22, No~10, p.~1387-1398. ISSN~0959-6658. Available from: https://dx.doi.org/10.1093/glycob/cws105.

Detailed Information on Publication Record