HAMMEROVÁ, Jindřiška, Stjepan ULDRIJAN, Eva TÁBORSKÁ, Alena HYRŠLOVÁ VACULOVÁ and Iva SLANINOVÁ. Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine. Biological Chemistry. Berlin, DE: Walter de Gruyter&CO, 2012, vol. 393, No 7, p. 647-658. ISSN 1431-6730. doi:10.1515/hsz-2011-0279.
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Basic information
Original name Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine
Authors HAMMEROVÁ, Jindřiška (203 Czech Republic, guarantor, belonging to the institution), Stjepan ULDRIJAN (203 Czech Republic, belonging to the institution), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution), Alena HYRŠLOVÁ VACULOVÁ (203 Czech Republic) and Iva SLANINOVÁ (203 Czech Republic, belonging to the institution).
Edition Biological Chemistry, Berlin, DE, Walter de Gruyter&CO, 2012, 1431-6730.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.683
RIV identification code RIV/00216224:14110/12:00057556
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1515/hsz-2011-0279
UT WoS 000307087600011
Keywords in English apoptosis; Bcl-2; benzophenanthridine alkaloids; LC3; necrostatin-1; RIP1
Tags International impact
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 4/9/2012 14:01.
Abstract
We show that the plant quaternary benzo[c]phenanthridine alkaloid sanguilutine (SL) is a strong inducer of caspase-independent non-apoptotic death in human melanoma cells. Necrostatin-1, a specific inhibitor of necroptosis, completely reversed the cytotoxic effect of SL, suggesting that necroptosis was a predominant type of cell death induced by SL in these cells. In addition, we showed that SL can trigger an autophagic response, as confirmed by GFP-LC3 puncta formation and LC3-II accumulation. Interestingly, we observed a significant decrease in the viability of melanoma cells treated with combination of autophagy inhibitors (3-methyladenine, bafilomycin-A1 and LY294002) and SL. Our results further indicated that autophagy may serve as a pro-survival mechanism, delaying the induction of necroptosis in melanoma cells. The ability of SL to induce caspase-independent non-apoptotic cell death (necroptosis) suggests its possible therapeutic potential in the treatment of apoptosis-resistant melanoma tumours. Furthermore, SL might serve as a useful tool for studying the mechanisms of necroptosis and autophagy induction and the interplay between these two processes.
Links
GA525/08/0819, research and development projectName: Rostlinné zdroje minoritních benzofenanthridinových alkaloidů a studium interakcí těchto alkaloidů s DNA
Investor: Czech Science Foundation, Standard Projects
LC06077, research and development projectName: Centrum chemické genetiky
Investor: Ministry of Education, Youth and Sports of the CR, Basic Research Center
LH12176, research and development projectName: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)
Investor: Ministry of Education, Youth and Sports of the CR, KONTAKT II
NS10236, research and development projectName: Terapeutický potenciál inhibice vybraných signálních drah v buňkách maligního melanomu
Investor: Ministry of Health of the CR, Departmental Programme of Research and Development - MH II from 2008 to 2011
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