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@article{990831,
author = {Hammerová, Jindřiška and Uldrijan, Stjepan and Táborská, Eva and Hyršlová Vaculová, Alena and Slaninová, Iva},
article_location = {Berlin, DE},
article_number = {7},
doi = {http://dx.doi.org/10.1515/hsz-2011-0279},
keywords = {apoptosis; Bcl-2; benzophenanthridine alkaloids; LC3; necrostatin-1; RIP1},
language = {eng},
issn = {1431-6730},
journal = {Biological Chemistry},
title = {Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine},
volume = {393},
year = {2012}
}
TY - JOUR
ID - 990831
AU - Hammerová, Jindřiška - Uldrijan, Stjepan - Táborská, Eva - Hyršlová Vaculová, Alena - Slaninová, Iva
PY - 2012
TI - Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine
JF - Biological Chemistry
VL - 393
IS - 7
SP - 647-658
EP - 647-658
PB - Walter de Gruyter&CO
SN - 14316730
KW - apoptosis
KW - Bcl-2
KW - benzophenanthridine alkaloids
KW - LC3
KW - necrostatin-1
KW - RIP1
N2 - We show that the plant quaternary benzo[c]phenanthridine alkaloid sanguilutine (SL) is a strong inducer of caspase-independent non-apoptotic death in human melanoma cells. Necrostatin-1, a specific inhibitor of necroptosis, completely reversed the cytotoxic effect of SL, suggesting that necroptosis was a predominant type of cell death induced by SL in these cells. In addition, we showed that SL can trigger an autophagic response, as confirmed by GFP-LC3 puncta formation and LC3-II accumulation. Interestingly, we observed a significant decrease in the viability of melanoma cells treated with combination of autophagy inhibitors (3-methyladenine, bafilomycin-A1 and LY294002) and SL. Our results further indicated that autophagy may serve as a pro-survival mechanism, delaying the induction of necroptosis in melanoma cells. The ability of SL to induce caspase-independent non-apoptotic cell death (necroptosis) suggests its possible therapeutic potential in the treatment of apoptosis-resistant melanoma tumours. Furthermore, SL might serve as a useful tool for studying the mechanisms of necroptosis and autophagy induction and the interplay between these two processes.
ER -
HAMMEROVÁ, Jindřiška, Stjepan ULDRIJAN, Eva TÁBORSKÁ, Alena HYRŠLOVÁ VACULOVÁ and Iva SLANINOVÁ. Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine. \textit{Biological Chemistry}. Berlin, DE: Walter de Gruyter\&{}CO, vol.~393, No~7, p.~647-658. ISSN~1431-6730. doi:10.1515/hsz-2011-0279. 2012.
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