Detailed Information on Publication Record
2012
Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine
HAMMEROVÁ, Jindřiška, Stjepan ULDRIJAN, Eva TÁBORSKÁ, Alena HYRŠLOVÁ VACULOVÁ, Iva SLANINOVÁ et. al.Basic information
Original name
Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine
Authors
HAMMEROVÁ, Jindřiška (203 Czech Republic, guarantor, belonging to the institution), Stjepan ULDRIJAN (203 Czech Republic, belonging to the institution), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution), Alena HYRŠLOVÁ VACULOVÁ (203 Czech Republic) and Iva SLANINOVÁ (203 Czech Republic, belonging to the institution)
Edition
Biological Chemistry, Berlin, DE, Walter de Gruyter&CO, 2012, 1431-6730
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.683
RIV identification code
RIV/00216224:14110/12:00057556
Organization unit
Faculty of Medicine
UT WoS
000307087600011
Keywords in English
apoptosis; Bcl-2; benzophenanthridine alkaloids; LC3; necrostatin-1; RIP1
Tags
International impact
Změněno: 4/9/2012 14:01, Mgr. Michal Petr
Abstract
V originále
We show that the plant quaternary benzo[c]phenanthridine alkaloid sanguilutine (SL) is a strong inducer of caspase-independent non-apoptotic death in human melanoma cells. Necrostatin-1, a specific inhibitor of necroptosis, completely reversed the cytotoxic effect of SL, suggesting that necroptosis was a predominant type of cell death induced by SL in these cells. In addition, we showed that SL can trigger an autophagic response, as confirmed by GFP-LC3 puncta formation and LC3-II accumulation. Interestingly, we observed a significant decrease in the viability of melanoma cells treated with combination of autophagy inhibitors (3-methyladenine, bafilomycin-A1 and LY294002) and SL. Our results further indicated that autophagy may serve as a pro-survival mechanism, delaying the induction of necroptosis in melanoma cells. The ability of SL to induce caspase-independent non-apoptotic cell death (necroptosis) suggests its possible therapeutic potential in the treatment of apoptosis-resistant melanoma tumours. Furthermore, SL might serve as a useful tool for studying the mechanisms of necroptosis and autophagy induction and the interplay between these two processes.
Links
| GA525/08/0819, research and development project |
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| LC06077, research and development project |
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| LH12176, research and development project |
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| NS10236, research and development project |
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