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@proceedings{991683,
author = {Pešl, Martin and Skopalík, Josef and Štěpán, Jiří and Ihnatová, Ivana and Starčuk, Zenon and Prášek, Jiří},
booktitle = {3rd International Congress on Stem Cells and Tissue Formation},
title = {Labeling of rabbit bone-marrow mesenchymal cells with In-111 and NMR contrast particles},
year = {2010}
}
TY - CONF
ID - 991683
AU - Pešl, Martin - Skopalík, Josef - Štěpán, Jiří - Ihnatová, Ivana - Starčuk, Zenon - Prášek, Jiří
PY - 2010
TI - Labeling of rabbit bone-marrow mesenchymal cells with In-111 and NMR contrast particles
N2 - Background and objectives: Bone-marrow mesenchymal cells (BMMCs) labeling methods were already described for canine, pig and rat models. Evaluation and comparison of labeling methods for rabbit BMMC is still lacking. Radioactive labeling with 111In and NMR contrast particles could be a method for tracking of cells delivered into the infarcted heart. Methods: Rabbit BMMCs were isolated and cultured for 1-3 weeks. (A) Labeling was based on incubation of cells with complex 111In-tropolone for 5-15 min. Labeling efficiency was determined. Surviving of BMMCs during 1 week was monitored. Different amount of labeled cells was placed in phantom of rabbit chest and underwent basic gamma camera imaging. (B) Two types of iron oxide particles (Resovist or supermagnetic maghemite) were added to the BMMCs culture up to final concentration 100ug/ml. After several days particles were washed out and labeled BMMCs placed in phantom of rabbit chest. NMR imaging was evaluated. Results: (A) Radioactive labeling efficiency was up to 40%, which resulted in BMMCs displaying about 3 Bq/cell. Viability was not significantly decreased by this procedure. BMMCs numbers as low as 50 x 10^3 could be easily localized and imaged using gamma camera. (B) Labeling with Resovist and deadherence process resulted in surviving of 80% of cells. Cluster as small as 50 x 10^3 cells was able to be detected by NMR imaging. Conclusion: Method of 111In and Resovist labeling of rabbit BMMCs were proved working and optimized. This method can be used as base for in vivo studies of tracking of cells delivered into the infarcted rabbit heart. Several weeks monitoring of labeled BMMCs (with typical problem as so called washout of radioactive/contrast agents etc.) is intensively monitored now.
ER -
PEŠL, Martin, Josef SKOPALÍK, Jiří ŠTĚPÁN, Ivana IHNATOVÁ, Zenon STARČUK a Jiří PRÁŠEK. Labeling of rabbit bone-marrow mesenchymal cells with In-111 and NMR contrast particles. In \textit{3rd International Congress on Stem Cells and Tissue Formation}. 2010.
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