Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella

BÜYÜKGÜZEL, Ender, Pavel HYRŠL a Kemal BÜYÜKGÜZEL. Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella. In XXIV International Congress of Entomology. 2012.

Základní údaje

• Originální název: Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella
• Název česky: Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella
• Autoři: BÜYÜKGÜZEL, Ender (792 Turecko), Pavel HYRŠL (203 Česká republika, garant, domácí) a Kemal BÜYÜKGÜZEL (203 Česká republika).
• Vydání: XXIV International Congress of Entomology, 2012.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 30102 Immunology
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/12:00060850
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova česky: Eicosanoids; Galleria mellonella; Antioxidant enzymes; Oxidative stress
• Klíčová slova anglicky: Eicosanoids; Galleria mellonella; Antioxidant enzymes; Oxidative stress
• Příznaky: Mezinárodní význam

Anotace

Antioxidant enzymes play a major role in the defense against pro-oxidative effects of xenobiotics and prooxidant plant allelochemicals in insects. We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with esculetin (0.001%), dexamethasone (0.001%) and phenidone (0.1%) to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing 0.05% xanthotoxin (XA) for 2 days. High dietary XA concentrations (0.005 and 0.1%) were associated with increasing MDA and carbonyl content (by 3-fold) and antioxidant enzyme activities, superoxide dismutase (SOD) (by 3-fold) and catalase (CAT) (by 4-fold), and glutathione-dependent enzymes, glutathione S-transferase (GST) (by 15-fold) and glutathione peroxidase (GPx) (by 7-fold). Relative to control, eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone also resulted in impaired MDA content and antioxidant enzyme activities. However, carbonyl content did not differ between control- and EBIs-feeding larvae. This oxidative stress was associated with elicited antioxidative responses by increasing SOD, GST and GPx and decreasing CAT activities in hemolymph. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants.

Anotace česky

Antioxidant enzymes play a major role in the defense against pro-oxidative effects of xenobiotics and prooxidant plant allelochemicals in insects. We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with esculetin (0.001%), dexamethasone (0.001%) and phenidone (0.1%) to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing 0.05% xanthotoxin (XA) for 2 days. High dietary XA concentrations (0.005 and 0.1%) were associated with increasing MDA and carbonyl content (by 3-fold) and antioxidant enzyme activities, superoxide dismutase (SOD) (by 3-fold) and catalase (CAT) (by 4-fold), and glutathione-dependent enzymes, glutathione S-transferase (GST) (by 15-fold) and glutathione peroxidase (GPx) (by 7-fold). Relative to control, eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone also resulted in impaired MDA content and antioxidant enzyme activities. However, carbonyl content did not differ between control- and EBIs-feeding larvae. This oxidative stress was associated with elicited antioxidative responses by increasing SOD, GST and GPx and decreasing CAT activities in hemolymph. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants.