JUNG, Yong Woo, J.S. BYUN, Y.D. KIM, Dušan HEMZAL and Josef HUMLÍČEK. Study of the Interaction Between HSA and Oligo-DNA Using Total Internal Reflection Ellipsometry. JOURNAL OF THE KOREAN PHYSICAL SOCIETY. Seoul: Korean Physical Soc, 2012, vol. 60, No 8, p. 1288-1291. ISSN 0374-4884. Available from: https://dx.doi.org/10.3938/jkps.60.1288.
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Basic information
Original name Study of the Interaction Between HSA and Oligo-DNA Using Total Internal Reflection Ellipsometry
Authors JUNG, Yong Woo (410 Republic of Korea), J.S. BYUN (410 Republic of Korea), Y.D. KIM (410 Republic of Korea), Dušan HEMZAL (203 Czech Republic, belonging to the institution) and Josef HUMLÍČEK (203 Czech Republic, guarantor, belonging to the institution).
Edition JOURNAL OF THE KOREAN PHYSICAL SOCIETY, Seoul, Korean Physical Soc, 2012, 0374-4884.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10302 Condensed matter physics
Country of publisher Republic of Korea
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.506
RIV identification code RIV/00216224:14740/12:00060852
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.3938/jkps.60.1288
UT WoS 000304100200020
Keywords in English Ellipsometry; Total internal reflection; HSA; DNA; Biosensors
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Josef Humlíček, CSc., učo 307. Changed: 6/1/2014 12:14.
Abstract
Techniques of quantitative analysis are very important for studies of the interactions between bio-molecules in the field of biotechnology and drug development. The total internal reflection ellipsometry system (TIRE) is an attractive label-free procedure for the quantitative analysis of biomolecules because it combines the analytic ability of ellipsometry and the high surface sensitivity of surface plasmon resonance. In this work, we have used TIRE to study the optical properties of an aquatic monolayer of human serum albumin (HSA) and oligo-DNA. Also, we have monitored the adsorption and the interaction processes of protein layers.
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