J 2012

Study of the Interaction Between HSA and Oligo-DNA Using Total Internal Reflection Ellipsometry

JUNG, Yong Woo, J.S. BYUN, Y.D. KIM, Dušan HEMZAL, Josef HUMLÍČEK et. al.

Basic information

Original name

Study of the Interaction Between HSA and Oligo-DNA Using Total Internal Reflection Ellipsometry

Authors

JUNG, Yong Woo (410 Republic of Korea), J.S. BYUN (410 Republic of Korea), Y.D. KIM (410 Republic of Korea), Dušan HEMZAL (203 Czech Republic, belonging to the institution) and Josef HUMLÍČEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF THE KOREAN PHYSICAL SOCIETY, Seoul, Korean Physical Soc, 2012, 0374-4884

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10302 Condensed matter physics

Country of publisher

Republic of Korea

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 0.506

RIV identification code

RIV/00216224:14740/12:00060852

Organization unit

Central European Institute of Technology

DOI

UT WoS

000304100200020

Keywords in English

Ellipsometry; Total internal reflection; HSA; DNA; Biosensors

Tags

Tags

International impact, Reviewed
Změněno: 6/1/2014 12:14, prof. RNDr. Josef Humlíček, CSc.

Abstract

V originále

Techniques of quantitative analysis are very important for studies of the interactions between bio-molecules in the field of biotechnology and drug development. The total internal reflection ellipsometry system (TIRE) is an attractive label-free procedure for the quantitative analysis of biomolecules because it combines the analytic ability of ellipsometry and the high surface sensitivity of surface plasmon resonance. In this work, we have used TIRE to study the optical properties of an aquatic monolayer of human serum albumin (HSA) and oligo-DNA. Also, we have monitored the adsorption and the interaction processes of protein layers.