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@proceedings{991823, author = {Mádr, Aleš and Musilová, Jindra and Crha, Igor and Glatz, Zdeněk}, booktitle = {29th International Symposium on Chromatography (ISC 2012, Torun)}, keywords = {culture media, assisted reproduction, capillary electrophoresis, acetonitrile stacking}, isbn = {978-83-7780-440-7}, title = {Analysis of the embryo culture media by capillary electrophoresis using acetonitrile stacking}, year = {2012} }
TY - CONF ID - 991823 AU - Mádr, Aleš - Musilová, Jindra - Crha, Igor - Glatz, Zdeněk PY - 2012 TI - Analysis of the embryo culture media by capillary electrophoresis using acetonitrile stacking SN - 9788377804407 KW - culture media, assisted reproduction, capillary electrophoresis, acetonitrile stacking N2 - In recent years, several techniques were applied for studies of the embryo metabolome regarding embryo viability assessment. They are the most important and challenging tasks in the assisted reproduction for pregnancy rates after embryo transfer are dependent on the quality or developmental potential of selected embryos. Besides other molecules, amino acids are essential for embryonic well-being. It was found that amino acids profiling could predict blastocyst formation and clinical pregnancy. However, utilization of analytical techniques measuring levels of amino acids in clinical laboratories is hampered by low clinical practicality and expensiveness. Capillary electrophoresis (CE) coupled with contactless conductivity detection is highly efficient analytical technique which can be miniaturized onto chip and be fully automatic. Acquisition and operating costs are acceptable, sample consumption is negligible. For measurement of amino acids differences, there are placed demands on sensitivity. CE offers a wide variety of online preconcentration techniques, e.g. acetonitrile stacking, thus sensitivity can be improved without handling with a sample. ER -
MÁDR, Aleš, Jindra MUSILOVÁ, Igor CRHA a Zdeněk GLATZ. Analysis of the embryo culture media by capillary electrophoresis using acetonitrile stacking. In \textit{29th International Symposium on Chromatography (ISC 2012, Torun)}. 2012. ISBN~978-83-7780-440-7.
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