BH3-ONLY PROTEINS - FROM STRUCTURE TO FUNCTION

IONESCU, Crina-Maria, Zuzana KLÍMOVÁ, David SEHNAL, Radka SVOBODOVÁ VAŘEKOVÁ and Jaroslav KOČA. BH3-ONLY PROTEINS - FROM STRUCTURE TO FUNCTION. In XXIII. biochemický sjezd České společnosti pro biochemii a molekulární biologii a Slovenské spoločnosti pre biochémiu a molekulárnu biológiu. 2012.

Basic information

• Original name: BH3-ONLY PROTEINS - FROM STRUCTURE TO FUNCTION
• Authors: IONESCU, Crina-Maria, Zuzana KLÍMOVÁ, David SEHNAL, Radka SVOBODOVÁ VAŘEKOVÁ and Jaroslav KOČA.
• Edition: XXIII. biochemický sjezd České společnosti pro biochemii a molekulární biologii a Slovenské spoločnosti pre biochémiu a molekulárnu biológiu, 2012.

Other information

• Type of outcome: Presentations at conferences
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Keywords in English: apoptosis, BH3 domain

Abstract

Disfunctions in the mechanism of programmed cell death (apoptosis) are directly related to cancer, neurodegenerative and auto-immune diseases. Apoptosis is highly regulated by the interplay of BH3-only proteins [1,2]. How these proteins perform their function at the molecular level is not yet understood, which makes it very hard to develop medical treatment. We recently developed SiteBinder, an effective tool for comparing the 3D structure of multiple protein motifs [3]. Using this tool, we performed a brief structural comparison of the BH3 domain of several BH3-only proteins known to promote cell death. The preliminary results showed that there is a detectable structural trend which correlates with the type of mechanism of action (direct versus indirect) of these proteins. We present here our results of an extensive structural comparison analysis comprising all BH3-only proteins whose structure is known. Our investigations were ultimately meant to determine whether the measures of 3D structural similarity correlate with specificity of binding. The results of our analysis can be useful in studies focused on modulating apoptosis and related diseases.

Links

• GD301/09/H004, research and development project: Name: Molekulární a strukturní biologie vybraných cytostatik. Od mechanistických studií k chemoterapii rakoviny

Investor: Czech Science Foundation

• MUNI/A/0914/2009, interní kód MU: Name: Rozsáhlé výpočetní systémy: modely, aplikace a verifikace (Acronym: SV-FI MAV)

Investor: Masaryk University, Category A