Další formáty:
BibTeX
LaTeX
RIS
@article{1115115,
author = {Čejková, Darina and Zobaníková, Marie and Pospíšilová, Petra and Strouhal, Michal and Mikalová, Lenka and Weinstock, George M. and Šmajs, David},
article_location = {Reading},
article_number = {2},
doi = {http://dx.doi.org/10.1099/jmm.0.050658-0},
keywords = {article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi},
language = {eng},
issn = {0022-2615},
journal = {Journal of Medical Microbiology},
title = {Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains},
volume = {62},
year = {2013}
}
TY - JOUR
ID - 1115115
AU - Čejková, Darina - Zobaníková, Marie - Pospíšilová, Petra - Strouhal, Michal - Mikalová, Lenka - Weinstock, George M. - Šmajs, David
PY - 2013
TI - Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
JF - Journal of Medical Microbiology
VL - 62
IS - 2
SP - 196-207
EP - 196-207
PB - Society for General Microbiology
SN - 00222615
KW - article
KW - bacterial strain
KW - bacterium isolate
KW - controlled study
KW - gene deletion
KW - genotype
KW - nonhuman
KW - operon
KW - polymerase chain reaction
KW - priority journal
KW - RNA sequence
KW - Treponema
KW - Treponema pallidum
KW - Treponema paraluiscuniculi
N2 - This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/ subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.
ER -
ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lenka MIKALOVÁ, George M. WEINSTOCK a David ŠMAJS. Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains. \textit{Journal of Medical Microbiology}. Reading: Society for General Microbiology, roč.~62, č.~2, s.~196-207. ISSN~0022-2615. doi:10.1099/jmm.0.050658-0. 2013.
|
