This diploma thesis is focused on detection of the relationship between the reaction-speed ability and agility with players pupil categories FC Sparta Brno by using the device Fitro Agility Check. The first part of this thesis describes a comprehensive overview of modern football, sports performance in football, concretize the concept of agility and show his constituents. The second part deals with the analysis and evaluation of the result measurements. This work can serve as a basis for further research or as information resource for football coaches.