The bachelor's thesis „Human sperm DNA and its disturbances“
will concern with published data about the mechanisms of DNA
breaks present in the human sperm head and the possible causes
and the time and place of their origin.
Further the present knowledge about the influence of the DNA
breaks onto the development of human embryo and reparation
possibilities and abilities of fertilized egg. Among the
current methods of the DNA breaks detection belongs TUNEL
assay, SCSA test, SCD test and comet assay. Possibilities of
the separation of viable spermatozoa from those with high
frequency of breaks will be also described.
DNA sperm damage may cause a difference between fertile and
infertile man and this is also why current methods of assisted
reproductive treatment deal with this problem.