This Bachelor thesis deals with physical conditional preparation of football referee. An aim of the thesis is to determine an influence of intervention program on a level of physical condition of the tested referees in form of a case study. The thesis is created based on theoretical knowledge acquired from the literature and other relevant sources, and also based on research part, i.e. by the intervention program. A practical part describes the whole process of the intervention program and also the obtained results. The next part deals with an evaluation of tested individuals by discussion and summarization of the whole intervention program. Based on the results of the thesis propose additional possible directions of the future research.