The aim of the thesis was to investigate the relation between personality factors and factors of learning styles of high school students. Partial goals were to investigate relation of personality factors or learning styles with school achievement and to validate gender differences in learning styles and personality factors. Data collection was carried out using a questionnaire LSI (R. Dunn, K. Dunn, G. Price) for the learning styles and NEO-FFI (Costa PT, McCrae RR) for the personality factors. The research sample consisted of a total of 219 secondary school students and female students. The results indicated that there were significant, although not very strong correlation between learning styles and personality factors that were significantly influenced by gender. The relationship between school achievement and personality factors were found out only for Conscientiousness factor, and only for boys. School achievement most significantly correlated with persistence and responsibility factors. No significant gender differences in the learning styles were found out. Gender differences in personality factors were partially proved for the factor of Neuroticism and Openness. The causes of these findings were discussed.