The lichens are poikilohydric organisms which are very resistant against the stress caused by radiation, especially against UV-B radiation. UV-B radiation has a negative effect on physiological functions of lichens. However, the lichnes developed some adaptive mechanisms to protect own thalli against harmful effects of UV-B radiation. I analyzed the content of UV-absorbing compounds and pigments by spectrophotometric methods in my Master thesis. In my experiment with controlled conditions with supplemental UV-B radiation, I found that the response of photosynthetic apparatus of different species of lichens, their photobionts, respectively, is species-specific. According to my experimental findings the different species of lichens in natural state contain some amount of UV-absorbing compounds. Synthesis of these compounds is induced by currently incident UV-B radiation. The amount of UV-absorbing compounds is increased by higher doses of UV-B radiation. Therefore, these compounds have a photoprotective function. The best known UV-absorbing compounds are the parietin and the usnic acid. In my experiments, supplemental UV-B radiation had a negative impact on the photosynthetic processes in lichens. I found that the parametres of chlorophyll fluorescence derived from Kautsky curve supplemented by saturation pulses and Chlorophyll Fluorescence Imaging technique (especially effective quantum yield ΦII), and fast induction kinetics decreased due to the impact of UV-B radiation. Similarly to UV-B absorbing compounds, the response of chlorophyll fluorescence parameters was species-specific.