Carboxypeptidase B1 is a highly tissue specific pancreatic
protein, whose elevated levels were observed on both protein
and transcriptomic level in breast cancer. Based on
proteomics-transcriptomics studies made on clinico-pathological
representative group of patients CPB1 was identified as
putative biomarker of grade 1 breast tumors with high risk of
metastases in the lymph nodes. Therefore, the aim of this
thesis is to improve the functional characterization of this
protein, clarifying its impact on migration and invasiveness of
tumor cells and attempt to prepare stably transfected cell
line. To verify the specificity of CPB1 for grade 1 breast
tumors, luminal type A, the analysis of online available data
in the Oncomine database and Kaplan-Maier estimate survival
curves were used. To prove a trend that CPB1 increases
migration and invasiveness of tumor cells Transwell and
xCELLigence system methods were used. Pilot proteomic
experiments evaluating the effect of CPB1 on changes of total
proteome and identifying potential interaction partners of this
protein were also carried out. Performed research is a
contribution to a better functional protein characterization of
CPB1 and is a good basis for the continuation and optimization
of methods used for this purpose.