Several projects focused on urinary tract-related catheter
biofilm composition are compiled in this thesis. Besides, the
suitability of molecular-based techniques for polymicrobial low
abundant sample analysis is presented. Both Polymerase Chain
Reaction-Denaturing Gradient Gel Electrophoresis followed by
Sequencing (PCR-DGGE-S) and routine culture were used to
characterize bacterial biofilms from urinary tract-related
catheters, whereas next-generation sequencing methods were
performed in selected samples. Culture and fungal ITS2
amplification followed by capillary electrophoresis were used
to characterize Candida spp. in catheter biofilms.
Possibilities of Candida parapsilosis species identification
and the importance of bacterial species Actinotignum schaalii
in catheterized patients were studied and broadly discussed.
The applicability of molecular techniques for clinical use was
proved in infective endocarditis suffering but culture-negative
patient.