The focus of this diploma thesis is optimization of capillary
isoelectric focusing method (CIEF) for its future utilization
in the two-dimensional proteomics analysis. The thesis deals
with the capillary coating proto-col and optimization of the
separation of tryptic digest of several pro-teins including
BSA, myoglobin, and caseins. In the last part, the possi-bility
of CIEF-based sample fractionation is tested.