Curriculum Vitae

Curriculum Vitae

Person-Related Identification Information

• Mgr. Karel Kubíček, Ph.D., 19.09.1977 in Valtice, married, 2 children

Department

• Department of Condensed Matter Physics
  Masaryk University
  Kotlářská 2
  611 37 Brno
  Czech Republic
   
  
• CEITEC - Central European Institute of Technology
  Structural Biology of Gene Regulation
  Kamenice 753/5
  625 00 Brno
   
  T: +420 549 49 3253
  M: +420 731 28 76 16
  F: +420 541 211 214
  E: karelk(AT)physics.muni.cz
      karel.kubicek(AT)ceitec.muni.cz

Employment - Position

• Associate Professor in Biophysics
  
• Researcher @Laboratory of Structural Biology of Gene Regulation (prof. Richard Štefl)

Education and Academic Qualifications

• 2020-present: Associate Professor in Biophysics
• 2018 (5 months): Scientific exchange within Twinning project (TWINFUSYON) Johannes Kepler University Linz, Austria (prof. Kurt Hingerl)
• 2008-present: Researcher in the laboratory of prof. Richard Štefl
• 2007-2020: Assistant Professor for Biophysics at the Dept. Condensed Matter Physics, Faculty of Science, Masaryk University, Brno, Czech Republic
• 2005–2007: Postdoctoral researcher at the Max-Planck-Institute for Biophysical Chemistry in Göttingen, Department of NMR-based Structural Biology; studies of ligand–target interactions by solution NMR in the group of Dr. Teresa Carlomagno and Prof. Christian Griesinger.)
• 2001–2004: International doctorate in structural biology, Magnetic Resonance Center (CERM) in Florence, Italy. Degree awarded by University of Florence, Italy. Topic of Thesis: NMR Characterization of Protein–Protein and Protein–Cofactor Interactions (Supervisor Prof.Lucia Banci)
• 1996–2001: Masaryk University, Faculty of Science, Department of Theoretical and Physical Chemistry, final exams passed in June 2001. Topic of Thesis: Application of Residual Dipolar Interactions for Structure Determination of Biomolecules (Supervisor Prof. Vladimír Sklenář)
• 1992–1996: Technical College of Chemistry in Brno, final exams passed in 1996

Grants

• 2015-2018 GAČR standard grant
• 2011-2013 OP Vzdělávání pro konkurenceschopnost: Reforma a rozvoj výuky Biofyziky pro potřeby 21. století
• 2008-2010 GAČR postdoctoral grant

Teaching Activities

• 2013-present Organisation of Biophysical competition (http://physics.muni.cz/biophys/souteze.shtml)
• 2008-present Mentoring students
• 2008-present Teaching activities (biophysics, practical and theoretical NMR spectroscopy, experimental techniques in biophysical research)

Scientific and Research Activities

• Electron microscopy, NMR spectroscopy, AFM microscopy, protein-protein interactions, drug-design, protein-ligand interactions, SAXS, fluorescent anisotropy, isothermal titration calorimetry, molecular dynamics, electron microscopy ...

University Activities

• 2021-2023 Academic Senate of the Masaryk University Brno, Czech Rep.
• 2018-2020 Academic Senate of the Masaryk University Brno, Czech Rep.
• 2015-2017 Academic Senate of the Masaryk University Brno, Czech Rep.
• 2008-2011 Academic Senate of the Faculty of Science of the Masaryk University Brno, Czech Rep.
• 1998–2001 Academic Senate of the Masaryk University Brno, Czech Rep.
• 1996–2001 Academic Senate of the Faculty of Science of the Masaryk University Brno, Czech Rep.

Activities Outside University

• The Board of Biophysics @University of South Bohemia

Awards Related to Science and Research

• 2006–2007 DAAD scholarship within the program “Modern Applications of Biotechnology”
• 2001 Special prize of the Czech chemical society for the “Best presentation” on the Student scientific competition
• 2001 Chemistry section prize
• 2000 I. prize in the Student scientific competition
• 2000 Chemistry section prize

Major Publications

• BRÁZDA, Pavel, Ondrej ŠEDO, Karel KUBÍČEK and Richard ŠTEFL. Efficient and robust preparation of tyrosine phosphorylated intrinsically disordered proteins. BioTechniques. UNITED HOUSE, 2 ALBERT PL, LONDON N3 1QB: FUTURE SCI LTD, 2019, vol. 67, No 1, p. 16-22. ISSN 0736-6205. Available from: https://dx.doi.org/10.2144/btn-2019-0033. URL info
• JASNOVIDOVA, Olga, Magdaléna KREJČÍKOVÁ, Karel KUBÍČEK and Richard ŠTEFL. Structural insight into recognition of phosphorylated threonine-4 of RNA polymerase II C-terminal domain by Rtt103p. EMBO reports. Hoboken: Wiley-Blackwell, 2017, vol. 18, No 6, p. 906-913. ISSN 1469-221X. Available from: https://dx.doi.org/10.15252/embr.201643723. URL info
• JASNOVIDOVA, Olga, Tomáš KLUMPLER, Karel KUBÍČEK, Sergei KALYNYCH, Pavel PLEVKA and Richard ŠTEFL. Structure and dynamics of the RNAPII CTDsome with Rtt103. Proceeding of the National Academy of Sciences of the USA. The Academy, 2017, vol. 114, No 42, p. 11133-11138. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1712450114. URL info
• NEČASOVÁ, Ivona, Tomáš KLUMPLER, Karel KUBÍČEK and Ctirad HOFR. Revisiting the role of TRF2 basic domain in chromatin maintenance with the most recent structural and functional approaches. In EMBO Workshop Telomeric chromatin and telomere fragility. 2015. info
• BAČÍKOVÁ, Veronika, Josef PASULKA, Karel KUBÍČEK and Richard ŠTEFL. Structure and semi-sequence-specific RNA binding of Nrd1. Nucleic Acids Research. United Kingdom: Oxford University Press, 2014, vol. 42, No 12, p. 8024-8038. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gku446. URL info
• TUDEK, Agnieszka, Odil Porrua FUERTE, Tomasz KABZINSKI, Michael LIDSCHREIBER, Karel KUBÍČEK, Andrea FOŘTOVÁ, Francoise LACROUTE, Štěpánka VAŇÁČOVÁ, Patrick CRAMER, Richard ŠTEFL and Domenico LIBRI. Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation. Molecular Cell. CAMBRIDGE: CELL PRESS, 2014, vol. 55, No 3, p. 467-481. ISSN 1097-2765. Available from: https://dx.doi.org/10.1016/j.molcel.2014.05.031. URL info
• SUCHÁNKOVÁ, Tereza, Karel KUBÍČEK, Jana KAŠPÁRKOVÁ, Viktor BRABEC and Jiří KOZELKA. Platinum-DNA interstrand crosslinks: Molecular determinants of bending and unwinding of the double helix. Journal of Inorganic Biochemistry. New York, USA: Elsevier Science Inc, 2012, vol. 108, No 1, p. 69-79. ISSN 0162-0134. Available from: https://dx.doi.org/10.1016/j.jinorgbio.2011.09.025. info
• KUBÍČEK, Karel, Hana ČERNÁ, Peter HOLUB, Josef PASULKA, Dominika HROŠŠOVÁ, Frank LOEHR, Ctirad HOFR, Štěpánka VAŇÁČOVÁ and Richard ŠTEFL. Serine phosphorylation and proline isomerization in RNAP II CTD control recruitment of Nrd1. Genes & Development. New York: Cold Spring Harbor Laboratory Press, 2012, vol. 26, No 17, p. 1891-1896. ISSN 0890-9369. Available from: https://dx.doi.org/10.1101/gad.192781.112. URL info
• PORRUA-FUERTE, Odil, Fruzsina HÓBOR, Jocelyne BOULAY, Karel KUBÍČEK, Yves D’AUBENTON-CARAFA, Rajani Kanth GUDIPATI, Richard ŠTEFL and Domenico LIBRI. In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination. EMBO Journal. NEW YORK: NATURE PUBLISHING GROUP, 2012, vol. 31, No 19, p. 3935-3948. ISSN 0261-4189. Available from: https://dx.doi.org/10.1038/emboj.2012.237. URL info
• ŠIKORSKÝ, Tomáš, Fruzsina HÓBOR, Eva KRIŽANOVÁ, Josef PASULKA, Karel KUBÍČEK and Richard ŠTEFL. Recognition of asymmetrically dimethylated arginine by TDRD3. Nucleic Acids Research. Oxford: Oxford University Press, 2012, vol. 40, No 22, p. 11748-11755. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gks929. URL info
• HÓBOR, Fruzsina, Roberto PERGOLI, Karel KUBÍČEK, Dominika HROŠŠOVÁ, Veronika BAČÍKOVÁ, Michal ZIMMERMANN, Josef PASULKA, Ctirad HOFR, Štěpánka VAŇÁČOVÁ and Richard ŠTEFL. Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein. The Journal of Biological Chemistry. Am. Soc. for Biochem. and Mol. Biol., 2011, vol. 286, No 5, p. 3645-3657. ISSN 0021-9258. Available from: https://dx.doi.org/10.1074/jbc.M110.158774. info
• KUBÍČEK, Karel, Josef PASULKA, Hana ČERNÁ, Frank LÖHR and Richard ŠTEFL. 1H, 13C, and 15N resonance assignments for the CTD-Interacting Domain of Nrd1 bound to Ser5-phosphorylated CTD of RNA Polymerase II. Biomolecular NMR Assignments. Netherlands: Springer Netherlands, 2011, vol. 5, No 2, p. 203-205. ISSN 1874-2718. Available from: https://dx.doi.org/10.1007/s12104-011-9300-y. URL info
• KUBÍČEK, Karel, Jordan MONNET, Simone SCINTILLA, Jana KOPEČNÁ, Fabio ARNESANO, Lukáš TRANTÍREK, Claude CHOPARD, Giovanni NATILE and Jiří KOZELKA. Unusual Interstrand Pt(S,S-diaminocyclohexane)-GG Crosslink Formed by Rearrangement of a Classical Intrastrand Crosslink Within a DNA Duplex. Chemistry - An Asian Journal. WILEY-V C H VERLAG GMBH, 2010, vol. 5, No 2, p. 244-247. ISSN 1861-4728. URL info
• PERGOLI, Roberto, Karel KUBÍČEK, Fruzsina HÓBOR, Josef PASULKA and Richard ŠTEFL. (1)H, (13)C, and (15)N chemical shift assignments for the RNA recognition motif of Nab3. Biomolecular NMR Assignments. UNITED STATES: SPRINGER, 2010, vol. 4, No 1, p. 119-121. ISSN 1874-2718. Available from: https://dx.doi.org/10.1007/s12104-010-9221-1. URL info
• KUBICEK, Karel, S. Kaspar GRIMM, Julien ORTS, Florenz SASSE and Teresa CARLOMAGNO. The Tubulin-Bound Structure of the Antimitotic Drug Tubulysin. Angewandte Chemie International Edition. Weinheim, Spolková republika Německo: Wiley-VCH Verlag GmbH & Co., 2010, vol. 49, No 28, p. 4809-4812. ISSN 1433-7851. URL info
• REESE, Marcel, Victor M SANCHEZ-PEDREGAL, Karel KUBÍČEK, Jens MEILER, Marcel J BLOMMERS, Christian GRIESINGER and Teresa CARLOMAGNO. Structural basis of the activity of the microtubule-stabilizing agent epothilone a studied by NMR spectroscopy in solution. Angewandte Chemie International Edition. Weinheim: Wiley-VCH Verlag GmbH & Co., 2007, vol. 46, No 11, p. 1864-1868. ISSN 1433-7851. URL info
• SANCHEZ-PEDREGAL, Victor M, Karel KUBÍČEK, Jens MEILER, Isabelle LYOTHIER, Ian PATERSON and Teresa CARLOMAGNO. The tubulin-bound conformation of discodermolide derived by NMR studies in solution supports a common pharmacophore model for epothilone and discodermolide. Angewandte Chemie International Edition. Weinheim: Wiley-VCH Verlag GmbH & Co., 2006, vol. 45, No 44, p. 7388-7394. ISSN 1433-7851. URL info
• BANCI, Lucia, Ivano BERTINI, Simone CIOFI-BAFFONI, Efthalia KATSARI, Nikolaos KATSAROS, Karel KUBÍČEK and Stefano MANGANI. A copper(I) protein possibly involved in the assembly of CuA center of bacterial cytochrome c oxidase. Proceedings of the National Academy of Sciences of the USA. Washington, 2005, vol. 102, No 11, p. 3994-3999. ISSN 0027-8424. URL info
• BANCI, Lucia, Ivano BERTINI, Isabella C FELLI, Ludwig KRIPPAHL, Karel KUBÍČEK, Jose J MOURA and Antonio ROSATO. A further investigation of the cytochrome b5-cytochrome c complex. Journal of Biological Inorganic Chemistry. Heidelberg: Springer Verlag, 2003, vol. 8, No 7, p. 777-786. ISSN 0949-8257. URL info

2021/06/07