Životopis

Curriculum vitae

Person Identification

• Jan Havliš, born December 17, 1970 in Brno.

Workplace

• Masaryk University
  : Faculty of Science
  :: National Centre for Biomolecular Research
  ::: Laboratory of functional Genomics and Proteomics
  Kamenice 5, CZ-6250 00 Brno

Employment Position

• Associate professor

Education and Academic Qualifications

• 2013: General Linguistics, B.A., "Construction of quasi-natural language - basic concept", Dept Gen Ling & Baltistics, Fac Art, Masaryk Uni
• 2006: Analytical Chemistry, habilitation, "Bioanalytics - HPLC and MS", Dept Analyt Chem, Fac Sci, Masaryk Uni
• 1998: Physical and Theoretical Chemistry, Ph.D., "Noval study of the redox properties of NAD-coenzymes", Dept Phys Theor Chem, Fac Sci, Masaryk Uni
• 1994: Biochemistry, M.Sc., "Interaction of serum albumin with copper(II) ions", Dept Biochem, Fac Sci, Masaryk Uni
• 1992: Biochemistry, B.Sc., Dept Biochem, Fac Sci, Masaryk Uni

Employment Summary

• November 2011 - till yet: associate professor, CEITEC MU & NCBR Fac Sci, Masaryk Uni
• September 2007 - October 2011: associate professor, Inst Experim Biol, Fac Sci, Masaryk Uni
• May 2006 - August 2007: associate professor, Dept Analyt Chem, Fac Sci, Masaryk Uni
• February 1998 - April 2006: assistant protfessor, Dept Analyt Chem, Fac Sci, Masaryk Uni
• October 1997 - January 1998: reseach assistant, Dept Analyt Chem, Fac Sci, Masaryk Uni

Pedagogical Activities

• 1994-1997: Lab. exercises in physical chemistry
  1997-2007: Methods in chemical research (part HPLC), lab. exercises
  1999-till yet: Separation methods A - cze, lecture
  1999: Petr Marsalek, master's thesis - consultant, finished
  1999: Alice Kotulanova, master's thesis - consultant, finished
  1998: Hana Chroma, PhD thesis - consultant, finished
  1999-2002: Sona Humplikova, master's thesis - supervisor, finished
  1999-2007: Seminar II (for students of Analytical Chemistry)
  2000-2001: Alice Kotulanova, PhD thesis - consultant, unfinished
  2000-till yet: Bioanalytics I - cze, lecture
  2000: Lasers in chemistry, lecture
  2003: Analysis of organic compounds 2 - lecture and lab. exercises
  2003-2005: Roman Tomas, master's thesis - supervisor, finished
  2004-2007: Tomas Prosecky, master's thesis - supervisor, finished
  2004-2005: Hana Ovadová, bachelor's thesis - supervisor, finished
  2005-2007: Michal Horak, master's thesis - consultant, finished
  2005-till yet: Bioanalytics II - cze, lecture
  2005-2007: Hana Ovadova, master's thesis - supervisor, finished
  2006: Lenka Monincova, bachelor's thesis - supervisor, finished
  2006-till yet: Lenka Monincova, master's thesis - supervisor, finished
  2006-till yet: David Potesil, PhD thesis - supervisor, finished
  2007-till yet: Hana Ovadova, PhD thesis - supervisor, unfinished
  2007-till yet: Radek Pilny, PhD thesis - supervisor, unfinished
  2007-till yet: Monika Brodova, PhD thesis - supervisor, unfinished
  2008-till yet: Petra Staskova, bachelor's thesis - supervisor, finished
  2008-till yet: Separation methods B - cze, lecture
  2008-till yet: Discover the science I & II - cze, lectures
  2008-till yet: Bioanalytics I - eng, lecture
  2008-till yet: Bioanalytics II - eng, lecture
  2009-till yet: Separation methods A - eng, lecture
  2009-till yet: Separation methods B - eng, lecture
  2009-2011: Marcela Zupkova, master's thesis - supervisor, finished
  2009-2011: Jan Mlatecek, master's thesis - consultant, finished
  2009-2011: Petr Rasovsky, master's thesis - consultant, finished
  2011-2012: Kamil Mikulasek, bachelor's thesis - consultant, finished
  2012-till yet: Seminary BGP I
  2012-till yet: Proteomics - cze (parts Intro, Proteins & Peptides, Expression Proteomics), lecture
  2012-2014: Kamil Mikulasek, master's thesis - consultant, finished
  2013-till yet: Seminary BGP II
  2013-till yet: Seminary BGP III
  2013-till yet: Methods in genomics and proteomics - cze (part Separation of Proteins), lecture
  2013-2015: Tomas Rozman, master's thesis - consultant, finished
  2014-till yet: Seminary BGP IV
  2014-till yet: Constructed languages & the phenomenon of language construction - cze, lecture
  2014-till yet: Law, ethics and philosophy of science (parts Philosophy of Science, Ethics in Science)- eng, lecture
  2014-till yet: Kamil Mikulasek, PhD thesis - supervisor
  2014-2015: Jan Fiala, bachelor's thesis - consultant, finished
  2015-till yet: Introduction to interlinguistics - cze, lecture
  2015-2017: Jan Fiala, master's thesis - consultant, finished
  2016-till yet: Proteomics - eng (parts Intro, Proteins & Peptides, Expression Proteomics), lecture
  2016-till yet: Discover the science I & II - eng, lectures
  2017-till yet: Methods in genomics and proteomics - eng (part Separation of Proteins), lecture
  

Scientific and Research Activities

• 1990-1994: Interaction of serum albumin with metal ions (Cu, Ni, Zn, Fe)
  1994-1998: Study of the redox properties of NAD-coenzymes
  1998-2007: MALDI TOF MS - quantification
  1998-2003: Determination of purins and pyrimidins by means of MALDI TOF MS in biol. samples as markers of gen. diseases
  1999-2001: Demethylation state of DNA within FRM1 gene
  2001-2003: Analyses of important substrates and metabolites of some diaminooxidases
  2001-2011: Study of protein complexes; Metalloproteins
  2001-till yet: Quantification of proteins and peptides using MS
  2003-till yet: Constructed languages and interlinguistics
  2010-till yet: QSRR of ultra short oligonucleotides in II-RPLC & HILIC
  

Internship and stays for the purpose of study or work

• February 1998: University of Vienna (Universitaet Wien), Faculty of Natural Sciences and Mathematics, Department of Analytical Chemistry, Bio Mass Spec, Austria, Dr Guenter Allmaier
  April 2001: University of Szeged (Szegedi Tudomanyegyetem), Faculty of Science, Department of Inorganic and Analytical Chemistry, Hungary, Dr Antal Peter
  2001-2003: Max Planck Institute, Molecular Cell Biology and Genetics, Mass Spectrometry Group, Dresden, Germany, Dr Andrej Shevchenko
  
• 2001/10/01 – 2003/09/29: Max-Planck-Institut für Molekulare Zellbiologie und Genetik, DEU
  • dvouletý postdoktorální pobyt zaměřený na výzkum v oblasti proteomiky

University Activities

• member of Doctoral Board in Life Sciences
  member Field Committee Genomics and Proteomics
  chair of of Field Committee Bio-omics
  since 2017 - senator of the AS of Fac Sci, Masaryk Uni
  since 2018 - deputy for pedagogical matters, NCBR

Extrauniversity Activities

• 2006-2011: secretary of Czech Chemical Society (CSCH), branch Brno
  2010-2015: member of Executive Board of Czech Society for Mass Spectrometry
  

Selected Publications

• MIKULÁŠEK, Kamil, Kamil JAROŇ, Petr KULHÁNEK, Miroslava BITTOVÁ a Jan HAVLIŠ. Sequence-dependent separation of trinucleotides by ion-interaction reversed-phase liquid chromatography A structure-retention study assisted by soft-modelling and molecular dynamics. Journal of Chromatography A. Amsterdam: Elsevier Science BV, 2016, roč. 1469, October, s. 88-95. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2016.09.060. supplementary data info
• HAVLIŠ, Jan, Tatiana ŠTOSOVÁ, Marek ŠEBELA, Pavel ŘEHULKA, Ondrej ŠEDO a Zbyněk ZDRÁHAL. Evaluation of the possible proteomic application of trypsin from Streptomyces griseus. Analytical Biochemistry. San Diego: Academic Press, 2008, roč. 376, č. 1, s. 94-102. ISSN 0003-2697. info
• HAVLIŠ, Jan a Mathias MANN. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols. U.S.A.: Nature Publishing Group, 2008, roč. 1, č. 6, s. 2856-2860. ISSN 1745-2473. info
• HAVLIŠ, Jan a Christine WALCH-SOLIMENA. Nucleo-cytoplasmic shuttling of the Golgi phosphatidylinositol 4-kinase Pik1 is regulated by 14-3-3 proteins and coordinates Golgi function with cell growth. Molecular and Cellular Biology. Washington, D.C.: ASM, 2008, roč. 19, č. 3, s. 1046-61, 16 s. ISSN 0270-7306. info
• POTĚŠIL, David, Soňa ČEJKOVÁ, Ludmila ROČŇOVÁ, Martin TRBUŠEK, Zbyněk ZDRÁHAL a Jan HAVLIŠ. Sensitization of B-CLL lymphocytes, quest for a protein marker. Which mass spectrometry quantitative technique will give answer? 2008. URL URL info
• HAVLIŠ, Jan. Speciace metaloproteinů - využití paralelně kombinovaných spektrometrických technik. In 13. Spektrometrická konference - sborník. 2007. info
• HAVLIŠ, Jan a Hana OVADOVÁ. METALO + PROTEIN = GE + LA + ICP + MS. In KANICKÝ, Viktor a Tomáš VACULOVIČ. 13. Spektrometrická konference - sborník. 2007. info
• HAVLIŠ, Jan. Analýza metaloproteinů pomocí MS - paralelně kombinované techniky GLIM. In 8. ročník Školy hmotnostní spektrometrie - Sborník přednášek. Brno: Fakulta Chemická, VUT, Brno, 2007, s. 100-108. ISBN 978-80-214-3477-6. info
• HAVLIŠ, Jan a Lenka MONINCOVÁ. Prediction of tryptic peptide retention times by means of soft modelling as a tool for liquid chromatography-mass spectrometry driven proteomics. Trends in Chromatography. Indie: Research Trends (P) Ltd., 2007, roč. 3, -, s. 65-72. ISSN 0972-8635. info
• HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Natalie WIELSCH, Henrik THOMAS, Zbyněk ZDRÁHAL a Andrej SHEVCHENKO. Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation. Proteomics. Weinheim: Wiley-VCH, 2006, roč. 10, č. 6, s. 2959-2963, 4 s. ISSN 1615-9853. info
• HAVLIŠ, Jan, Zbyněk ZDRÁHAL a Ondrej ŠEDO. Protein Modification Degree - An Indirect MS Quantitation Approach. In Abstract Book 17th IMSC Prague. 2006. info
• HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Petr TARKOWSKI, Natalie WIELSCH, Henrik THOMAS a Andrej SHEVCHENKO. Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process. In Abstract Book 17th IMSC Prague. 2006. info
• ŠEBELA, Marek, David KOPEČNÝ, Zbyněk LAMPLOT, Jan HAVLIŠ, Henrik THOMAS a Andrej SHEVCHENKO. Thermostable -cyclodextrin conjugates of two similar plant amine oxidases and their properties. Biotechnology and Applied Biochemistry. 2005, roč. 41, s. 77-84, 7 s. ISSN 0885-4513. info
• HAVLIŠ, Jan, Tobias OELSCHLAEGEL, Martin SCHWICKART, Joao MATOS, Aliona BOGDANOVA, Alain CAMASSES, Andrej SHEVCHENKO a Wolfgang ZACHARIAE. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Cell. United States of America: Cell Press, Cambridge, MA, USA, 2005, roč. 120, č. 6, s. 773-788, 15 s. ISSN 0092-8674. info
• HAVLIŠ, Jan, Ondrej ŠEDO a Zbyněk ZDRÁHAL. Indirect quantification of protein modification degree. In Book of abstracts 2nd Czech Proteomic Conference. 2005. info
• HAVLIŠ, Jan. Praktické aspekty absolutní kvantifikace proteinů a peptidů pomocí MS. In 3. ročník Školy HPLC/MS - Sborník přednášek. Pardubice: Univerzita Pardubice, 2005, s. 175-179, 4 s. ISBN 80-7194-785-7. info
• HAVLIŠ, Jan. Využití izotopového značení v hmotnostní spektrometrii. In 3. ročník Školy HPLC/MS - Sborník přednášek. Pardubice: Univerzita Pardubice, 2005, s. 147-153, 6 s. ISBN 80-7194-785-7. info
• HAVLIŠ, Jan, Klára NOVOTNÁ a Josef HAVEL. Optimization of high performance liquid chromatography separation of neuroprotective peptides. Fractional experimental design combined with Artificial neural networks. Journal of Chromatography A. Elsevier B.V., 2005, roč. 1096, 1-2, s. 50-57. ISSN 0021-9673. info
• HAVLIŠ, Jan, Tatiana ŠTOSOVÁ, Marek ŠEBELA a René LENOBL. Proteolytické enzymy: význam pro proteomiku. Chemické listy. Praha: Czech Chemical Society, 2005, roč. 99, č. 12, s. 896-905. ISSN 0009-2770. info
• THOMAS, Henrik, Jan HAVLIŠ, Jan PEYCHL a Andrej SHEVCHENKO. Dried-Droplet Probe Preparation on AnchorChip(TM) Targets for Navigating the Acquisition of MALDI TOF Spectra by Fluorescence of Matrix / Analyte Crystals. Rapid Communications in Mass Spectrometry. United Kingdom: John Wiley & Sons, Ltd, 2004, roč. 18, č. 9, s. 923-930. ISSN 0951-4198. info
• SCHWICKART, Martin, Jan HAVLIŠ, Bianca HABERMANN, Aliona BOGDANOVA, Alain CAMASSES, Tobias OELSCHLAEGEL, Andrej SHEVCHENKO a Wolfgang ZACHARIAE. Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27. Molecular and Cellular Biology. Washington, D.C.: ASM, 2004, roč. 24, č. 8, s. 3562-3576. ISSN 0270-7306. info
• HAVLIŠ, Jan a Andrej SHEVCHENKO. Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2004, roč. 76, č. 11, s. 3029-3036, 7 s. ISSN 0003-2700. info
• ŠEBELA, Marek, Zbyněk LAMPLOT, Michal MALOŇ, René LENOBEL, Karel LEMR, Jan HAVLIŠ, Pavel PEČ, Chunhua QIAO a Lawrence M. SAYRE. 1,5-Diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases. European Journal of Biochemistry. 2004, č. 271, s. 4696-4708, 12 s. ISSN 0014-2956. info
• HAVLIŠ, Jan, Henrik THOMAS, Marek ŠEBELA a Andrej SHEVCHENKO. Fast Response Proteomics by Accelerated In-gel Digestion of Proteins. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2003, roč. 75, č. 6, s. 1300-1306, 6 s. ISSN 0003-2700. info
• HAVLIŠ, Jan a Martin TRBUŠEK. 5-methyl-cytosine as a marker for monitoring of DNA methylation. Journal of Chromatography B. Amsterdam: Elsevier, 2002, roč. 781, 1-2, s. 373-391. ISSN 1570-0232. info
• HAVLIŠ, Jan, Anna SHEVCHENKO a Andrej SHEVCHENKO. Protein Identification by MALDI TOF MS: Practical Assessment of the Fast In-gel Digestion Protocol. 50th ASMS, Orlando, Florida, USA, 2002. info
• SHEVCHENKO, Anna, Daniel SCHAFT, Assen ROGUEV, Pim PIJNAPPEL, Jan HAVLIŠ, Francis STEWART a Andrej SHEVCHENKO. SEAMing Proteins from the TAP: Charting Protein Interaction Networks in Yeast by Tandem Affinity Purification and Mass Spectrometry. 50th ASMS, Orlando, Florida, USA, 2002. info
• HAVLIŠ, Jan, John Edward MADDEN, Alma Luísa REVILLA VÁZQUEZ a Josef HAVEL. High Performance Liquid Chromatographic determination of deoxycytidine monophosphate and methyl-deoxycytidine monophosphate for DNA demethylation monitoring: Experimental Design and Artificial Neural Networks optimisation. J. Chromatogr. B. Amsterdam: Elsevier Science, 2001, roč. 2001, č. 755, s. 185-193. ISSN 0378-4347. info
• CHROMÁ-KEULL, Hana, Jan HAVLIŠ a Josef HAVEL. Reactive Dye Ostazine Black V-B: Determination in Dye-bath and Hydrolysis Montoring by Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. Rapid Communications in Mass Spectrometry. United Kingdom: John Wiley & Sons, Ltd, 2000, roč. 2000, č. 14, s. 40-42. ISSN 0951-4198. info
• ŠEBELA, Marek, Pavel PEČ, Petr GALUSZKA a Jan HAVLIŠ. Characterisation of a homogenous plant aminoaldehyde dehydrogenase. Biochimica et Biophysica Acta. Amsterdam: Elsevier Science B.V., 2000, -, č. 1480, s. 329-669. ISSN 0005-2728. info
• CHROMÁ-KEULL, Hana, Jan HAVLIŠ a Josef HAVEL. : Reactive Dye Ostazine Black V-B: Determination in Dye-bath and Hydrolysis Monitoring by Matrix Assisted Laser Desorption-Ionisation Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 1999, roč. 13, č. 1, s. 40-43. ISSN 0951-4198. info
• HAVLIŠ, Jan, Alma Luísa REVILLA VÁZQUEZ, Libor KOZÁK a Josef HAVEL. Determination of purins, pyrimidines and their nucleosides in urine by HPLC and MALDI-TOF mass spectrometry. 23rd HPLC, Granada, Spain: n / a, 1999. n / a. info
• HAVLIŠ, Jan a Oldřich JANICZEK. Influence of protein-bound copper(II) on properties of bovine serum albumin-tryptophan complex. Scripta Facultatis Scientiarum Naturalium Universitatis Masarykianae - Chemistry. Brno: Přírodovědecká fakulta MU, 1998, roč. 1997-1998, 27-28, s. 43-49. ISSN 2100-6828. info
• HAVLIŠ, Jan a Marie STUDNICKOVA. Zinc centres in alcohol dehydrogenase from liver and from bakers yeast are metal dithiolenes. Biochemistry and bioenergeics : section of Journal of electroanalytical chemistry and electrochemistry. Lausanne: Elsevier Sequoia S.A., 1997, roč. 43, -, s. 157-159. ISSN 0302-4598. info
• HAVLIŠ, Jan, Oldřich JANICZEK a Martin MANDL. Influence of protein-bound copper(II) on properties of bovine serum albumin-tryptophan complex. In International Biometals Symposium. Calgary: University of Calgary, 1997, s. 25. info

2018/10/29