Curriculum Vitae

Životopis

Identifikace osoby

• Prof.RNDr.Igor Kučera,DrSc., narozen 1. ledna 1955 v Brně, ženatý, 2 děti

Pracoviště

• Katedra biochemie PřF MU
  Kotlářská 2
  611 37 Brno

Funkce na pracovišti

• profesor

Vzdělání a akademická kvalifikace

• 1998: Prof. biochemie
• 1992: Doc. biochemie, práce: "Oxid dusnatý v denitrifikační dráze bakterie Paracoccus denitrificans"
• 1990: DrSc. biochemie, práce: "Regulace denitrifikačních pochodů u bakterie Paracoccus denitrificans"
• 1979: RNDr. biochemie, práce: "Reakce katalysované alkoholdehydrogenasou v přítomnosti dvou substrátů a koenzymu - analytická aplikace a kinetika"

Přehled zaměstnání

• 1999-: profesor PřF MU
• 1992-1998: docent PřF MU
• 1985-1992: odborný asistent PřF UJEP/MU
• 1983-1985: odborný pracovník VV PřF UJEP
• 1979-1983: odborný asistent VV PřF UJEP
• 1978: odborný instruktor

Pedagogická činnost

• Přednášky: enzymologie, bioenergetika, nerovnovážné systémy
• Semináře: enzymologie, bioenergetika

Vědeckovýzkumná činnost

• Výzkumná činnost v oblasti mikrobiální bioenergetiky a enzymologie, se zaměřením na bakteriální denitrifikaci.
• GAČR 204/93/2054: Mechanismus regulace respiračních procesů u Paracoccus denitrificans
• GAČR 203/01/1989: Mechanismy regulace respiračních systémů denitrifikačních bakterií faktory prostředí

Universitní aktivity

• 2000-: vedoucí Sekce chemie PřF MU

Mimouniversitní aktivity

• 1996-2001: člen podoborové komise chemie Grantové agentury ČR
• 1993-2000: člen Vědecké rady Fakulty chemické VUT Brno

Ocenění vědeckou komunitou

• 1986: Plaketa Presidia Československé akademie věd
• 1984: Cena mladého biochemika Československé společnosti biochemické

Vybrané publikace

• FUKALA, Ivo and Igor KUČERA. Natural Polyhydroxyalkanoates-An Overview of Bacterial Production Methods. Molecules. Basel: MDPI, 2024, vol. 29, No 10, p. 1-25. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules29102293. URL info
• KRYL, Martin, Vojtěch SEDLÁČEK and Igor KUČERA. Structural Insight into Catalysis by the Flavin-Dependent NADH Oxidase (Pden_5119) of Paracoccus denitrificans. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2023, vol. 24, No 4, p. 1-17. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms24043732. URL info
• SEDLÁČEK, Vojtěch, Martin KRYL and Igor KUČERA. The ArsH Protein Product of the Paracoccus denitrificans ars Operon Has an Activity of Organoarsenic Reductase and Is Regulated by a Redox-Responsive Repressor. Antioxidants. Basel: MDPI, 2022, vol. 11, No 5, p. 1-18. ISSN 2076-3921. Available from: https://dx.doi.org/10.3390/antiox11050902. URL info
• KUČERA, Igor and Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. Microorganisms. Basel: MDPI, 2020, vol. 8, No 8, p. 1-11. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms8081230. URL info
• SEDLÁČEK, Vojtěch and Igor KUČERA. Arginine 95 is important for recruiting superoxide to the active site of the FerB flavoenzyme of Paracoccus denitrificans. FEBS Letters. Hoboken: Wiley, 2019, vol. 593, No 7, p. 697-702. ISSN 0014-5793. Available from: https://dx.doi.org/10.1002/1873-3468.13359. Full Text info
• SEDLÁČEK, Vojtěch and Igor KUČERA. Functional and mechanistic characterization of an atypical flavin reductase encoded by the pden_5119 gene in Paracoccus denitrificans. Molecular Microbiology. HOBOKEN: WILEY, 2019, vol. 112, No 1, p. 166-183. ISSN 0950-382X. Available from: https://dx.doi.org/10.1111/mmi.14260. URL info
• SEDLÁČEK, Vojtěch and Igor KUČERA. Modifications of the Aerobic Respiratory Chain of Paracoccus Denitrificans in Response to Superoxide Oxidative Stress. Microorganisms. Basel: MDPI, 2019, vol. 7, No 12, p. 1-12. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms7120640. Full Text info
• KUČERA, Igor and Vojtěch SEDLÁČEK. An Enzymatic Method for Methanol Quantification in Methanol/Ethanol Mixtures with a Microtiter Plate Fluorometer. Food Analytical Methods. New York: Springer, 2017, vol. 10, No 5, p. 1301-1307. ISSN 1936-9751. Available from: https://dx.doi.org/10.1007/s12161-016-0692-2. URL info
• SEDLÁČEK, Vojtěch, Tomáš KLUMPLER, Jaromír MAREK and Igor KUČERA. Biochemical properties and crystal structure of the flavin reductase FerA from Paracoccus denitrificans. Microbiological Research. ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2016, vol. 188, No 1, p. 9-22. ISSN 0944-5013. Available from: https://dx.doi.org/10.1016/j.micres.2016.04.006. URL info
• SEDLÁČEK, Vojtěch, Nikola PTÁČKOVÁ, Petra REJMONTOVÁ and Igor KUČERA. The flavoprotein FerB of Paracoccus denitrificans binds to membranes, reduces ubiquinone and superoxide, and acts as in vivo antioxidant. FEBS Journal. WILEY-BLACKWELL, 2015, vol. 282, No 2, p. 283-296. ISSN 1742-464X. Available from: https://dx.doi.org/10.1111/febs.13126. URL info
• PERNIKÁŘOVÁ, Vendula, Vojtěch SEDLÁČEK, David POTĚŠIL, Iva PROCHÁZKOVÁ, Zbyněk ZDRÁHAL, Pavel BOUCHAL and Igor KUČERA. Proteomic responses to a methyl viologen-induced oxidative stress in the wild type and FerB mutant strains of Paracoccus denitrificans. Journal of Proteomics. Amsterdam: ELSEVIER SCIENCE BV, 2015, vol. 125, July, p. 68-75. ISSN 1874-3919. Available from: https://dx.doi.org/10.1016/j.jprot.2015.05.002. URL info
• SEDLÁČEK, Vojtěch, Tomáš KLUMPLER, Jaromír MAREK and Igor KUČERA. The Structural and Functional Basis of Catalysis Mediated by NAD(P)H:acceptor Oxidoreductase (FerB) of Paracoccus denitrificans. PLOS ONE. Public Library of Science, 2014, vol. 9, No 5, p. 1-13. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0096262. URL info
• ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. Folia Microbiologica. Dordrecht, The Netherlands: Springer, 2011, vol. 56, No 1, p. 72-76. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-011-0004-6. URL info
• SEDLÁČEK, Vojtěch and Igor KUČERA. Chromate reductase activity of the Paracoccus denitrificans ferric reductase B (FerB) protein and its physiological relevance. Archives of microbiology. Springer, 2010, vol. 192, No 11, p. 919-926. ISSN 0302-8933. URL info
• ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA. Re-examination of the rationale for the use of bipyridylium cation radicals as site-specific electron donors for respiratory nitrate reduction. In FEBS Journal Supplement 33rd FEBS Congress. 2008. ISSN 1742-464X. info
• KUČERA, Igor. Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans. Microbiology-SGM. Reading, Berks (Great Britain): Society for General Microbiology, 2006, vol. 152, No 12, p. 3529-3534. ISSN 1350-0872. info
• KUČERA, Igor. Energy coupling to nitrate uptake into the denitrifying cells of Paracoccus denitrificans. Biochimica et Biophysica Acta Bioenergetics. Amsterdam: Elsevier, 2005, vol. 1709, No 2, p. 113-118. ISSN 0005-2728. URL info
• SEDLÁČEK, Vojtěch, Igor KUČERA and Oldřich JANICZEK. Purification and some new kinetic parameters of FerB enzyme from Paracoccus denitrificans. In ChemZi. Bratislava: Slovenská chemická společnost, 2005, p. 280-280. ISSN 1336-7242. info
• BOUCHAL, Pavel and Igor KUČERA. Examination of membrane protein expression in Paracoccus denitrificans by two-dimensional gel electrophoresis. Journal of Basic Microbiology. Weinheim, Germany: WILEY-VCH Verlag GmbH & Co., 2004, vol. 44, No 1, p. 17-22. ISSN 0233-111X. Publikace v pdf formátu info
• MAZOCH, Jiří, Radek TESAŘÍK, Vojtěch SEDLÁČEK, Igor KUČERA and Jaroslav TURÁNEK. Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans. European Journal of Biochemistry. Oxford: Blackwell Science, 2004, vol. 271, No 3, p. 553-562. ISSN 0014-2956. URL info
• KUŇÁK, Michal, Igor KUČERA and Rob J.M. VAN SPANNING. Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells. Archives of biochemistry and biophysics. New York: Academic Press, 2004, vol. 429, No 2, p. 237-243. ISSN 0003-9861. URL info
• KUČERA, Igor. Inhibition by phenylglyoxal of nitrate transport in Paracoccus denitrificans: a comparison with the effect of a protonophorous uncoupler. Archives of biochemistry and biophysics. New York: Academic Press, 2003, vol. 409, No 2, p. 327-334. ISSN 0003-9861. URL info
• KUČERA, Igor. Passive penetration of nitrate through the plasma membrane of Paracoccus denitrificans and its potentiation by the lipophilic tetraphenylphosphonium cation. Biochimica et Biophysica Acta. Amsterdam: Elsevier Science B.V., 2003, vol. 1557, No 1, p. 119-124. ISSN 0005-2728. URL info
• MAZOCH, Jiří, Michal KUŇÁK, Igor KUČERA and Rob VAN SPANNING. Fine-tuned regulation by oxygen and nitric oxide of the activity of a semi-synthetic FNR-dependent promoter and expression of denitrification enzymes in Paracoccus denitrificans. Microbiology. United Kingdom (Reading): Society for General Microbiology, UK, 2003, vol. 149, No 12, p. 3405-3412. ISSN 1350-0872. URL info
• KUČERA, Igor and Michal KUŇÁK. Steady-state kinetic analysis of substrate pair cycling between two enzymes: application to a mediated electron transport between the cytoplasmic membrane and the periplasmic nitrite reductase of Paracoccus denitrificans. Biophysical Chemistry. Elsevier, 2003, vol. 104, No 3, p. 617-622. ISSN 0301-4622. URL info

2016/01/29