Životopis

Curriculum vitae

Person Identification

• Asssoc. Prof. Mgr. Ctirad Hofr, Ph.D.

Workplace

• Functional Genomics and Proteomics – National Centre for Biomolecular Research
  Faculty of Science
  Masaryk University
  Kamenice 5, 625 00 Brno
  Czech Republic

Employment Position

• associate professor

Education and Academic Qualifications

• Assoc. Prof. , Biomolecular chemistry, Masaryk University 2015
• Ph.D., Biophysics, Masaryk University 2000
• M.Sc., Biophysics, Masaryk University 1997

Employment Summary

• Functional Genomics and Proteomics – National Centre for Biomolecular Research from 2019
  Faculty of Science, Masaryk University
• Scientific Incubator from 2019
  Institute of Biophysics, Academy of Sciences of the Czech Republic
• Chromatin Molecular Complexes 2011 – 2019
  CEITEC, Masaryk University, Brno
• Laboratory of Functional genomics and Proteomics from 2005
  Faculty of Science, Masaryk University, Brno
• Department of Chemistry and Chemical Biology 2002 – 2003
  Rutgers, The State University of New Jersey, New Brunswick, USA
• Laboratory of Molecular Biophysics and Pharmacology1995 - 2005
  Institute of Biophysics, Academy of Sciences of the Czech Republic

Pedagogical Activities

• C7230  Fluorescence Methods in Life Sciences from 2007
• C7235  Fluorescence Methods in Life Sciences - Practicals from 2007
• F9070  Experimental Methods in Biophysics from 2001
• F7790  Seminar in Biophysics and Biophysical Chemistry from 2011
• CG090 Methods in Proteomics from 2019
• Summer school on experimental biology 2010 - 2019

Scientific and Research Activities

• Applied biophysical methods in analyzing biomolecular interactions, the molecular mechanism of action of essential protein complexes affecting DNA transcription, telomerase activity in cancer cells, molecular principles of action of clinically used and novel drugs, studies of protein-DNA, protein-RNA, and protein-protein interactions. Continuous development of expertise in basic research-oriented towards medical applications.

Academical Stays

• Laboratory of Thomas Cech (Nobel Laureate), University of Colorado Boulder, Boulder USA 2013, 2014
• Rutgers, The State University of New Jersey, Department of Chemistry and Chemical Biology, New Brunswick, USA 2002-2003

University Activities

• Academic Senate of Masaryk University 2015 - 2017
• State Examination Committee of the Doctoral Study Programme - Genomics and Proteomics from 2019
• State Examination Committee of the Doctoral Study Programme - Bio-omics 2017 - 2020

Extrauniversity Activities

• Examination committee of the Study Programme Biomedical Engineering and Bioinformatics,Brno University of Technology 2017 - 2019
• Evaluation Panel of Biophysics and Macromolecular Physics, Grant Agency of the Czech Republic, 2017 - 2020
• Reviewering for Nucleic Acids Research, Nature Communications, Chemical Communications, Biophysical Journal, Journal of Biological Chemistry, and other journals from Royal Society of Chemistry Publishing.

Appreciation of Science Community

• King Rudolf II. Sholarship, Ministry of Education, Youth and Sports 2003-2004
• Award for Young Researcher of the Biophysical Institute 2001

Selected Publications

• KUČERA, Josef, Ondřej PEŠ, Tomáš JANOVIČ, Ctirad HOFR, Lenka KUBINYIOVÁ, Ján TÓTH, Štěpán KÁŇA a Petr TÁBORSKÝ. Enhancement of luminescence signal by deuterated water - Practical implications. Sensors and Actuators B: Chemical. Amsterdam: Elsevier B.V., roč. 352, č. 1, s. 1-8. ISSN 0925-4005. doi:10.1016/j.snb.2021.131029. 2022. URL info
• JANOVIČ, Tomáš, Martin STOJASPAL, Pavel VEVERKA a Ctirad HOFR. Assembly of human guardians of telomeres: TRF1 exchanges TRF2 bound to TIN2 but tolerates TRF2 in TPP1 presence. In CEITEC PhD Conference. 2021. URL info
• JANOVIČ, Tomáš, Tomáš BROM, Martin STOJASPAL, Pavel VEVERKA, Denisa HORÁKOVÁ a Ctirad HOFR. Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 When TPP1 Is Absent. In 26th International Workshop on “Single Molecule Spectroscopy and Super-resolution Microscopy”. 2021. URL info
• BROM, Tomáš, Martin STOJASPAL, Tomáš JANOVIČ, Pavel VEVERKA a Ctirad HOFR. Molecular mechanism of glioblastoma cell differentiation. In FEBS INSTRUCT MOBIEU Practical course: Hydrodynamic and ther- modynamic analysis of biological macromolecules and their interactions. 2020. info
• JANOVIČ, Tomáš a Ctirad HOFR. Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 when TPP1 Is Absent. In CEITEC PhD Conference. 2020. URL info
• JANOVIČ, Tomáš, Martin STOJASPAL, Pavel VEVERKA, Denisa HORÁKOVÁ a Ctirad HOFR. Human Telomere Repeat Binding Factor TRF1 Replaces TRF2 Bound to Shelterin Core Hub TIN2 when TPP1 Is Absent. Journal of Molecular Biology. London: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, roč. 431, č. 17, s. 3289-3301. ISSN 0022-2836. doi:10.1016/j.jmb.2019.05.038. 2019. Full Text info
• VEVERKA, Pavel, Tomáš JANOVIČ a Ctirad HOFR. Quantitative Biology of Human Shelterin and Telomerase: Searching for the Weakest Point. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, roč. 20, č. 13, s. 1-13. ISSN 1422-0067. doi:10.3390/ijms20133186. 2019. Full Text info
• HOFR, Ctirad, Ivona NEČASOVÁ, Eliška JANOUŠKOVÁ, Tomáš JANOVIČ, Martin STOJASPAL a Pavel VEVERKA. HOW TO TRAIN OUR CELLS TO BECOME YOUNGER – QUANTITATIVE BIOPHYSICS OF HUMAN TELOMERASE AND ITS GUARD SHELTERIN. In František Foret, Jana Křenková, Iveta Drobníková, Karel Klepárník, Jan Přikryl. 15 th International Interdisciplinary Meeting on Bioanalysis - Conference Proceedings. Česká republika: Institute of Analytical Chemistry of the CAS, v. v. i., Brno, Czech Republic. s. 59-63. ISBN 978-80-904959-7-5. 2018. URL info
• HOFR, Ctirad, Ivona NEČASOVÁ a Eliška JANOUŠKOVÁ. Rap1 and TRF2 in D-loop unwinding and telomere finding. In EMBO workshop, Telomere biology and human disease 1-6 May 2018, Tróia, Portugal. 2018. URL info
• NEČASOVÁ, Ivona, Eliška JANOUŠKOVÁ, Tomáš KLUMPLER a Ctirad HOFR. Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Research. roč. 45, č. 21, s. 12170-12180. ISSN 0305-1048. doi:10.1093/nar/gkx812. 2017. LifeB - Laboratory of interaction and function of essential biomolecules Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it info
• VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ, Ctirad HOFR a Karel ŘÍHA. Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. Plant Cell. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, roč. 29, č. 6, s. 1533-1545. ISSN 1040-4651. doi:10.1105/tpc.17.00064. 2017. URL info
• NEČASOVÁ, Ivona, Eliška JANOUŠKOVÁ, Tomáš KLUMPLER a Ctirad HOFR. Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it (gkx812) (vol 45, pg 12170, 2017). Oxford: Oxford University Press. doi:10.1093/nar/gkx968. 2017. info
• SEPSIOVA, Regina, Ivona NEČASOVÁ, Smaranda WILLCOX, Katarina PROCHAZKOVA, Peter GORILÁK, Jozef NOSEK, Ctirad HOFR, Jack D. GRIFFITH a Ĺubomír TOMÁŠKA. Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins. Plos one. San Francisco: Public Library of Science, roč. 11, č. 4, s. 1-17. ISSN 1932-6203. doi:10.1371/journal.pone.0154225. 2016. Full Text info
• DALBY, AB, Ctirad HOFR a TR CECH. Contributions of the TEL-patch Amino Acid Cluster on TPP1 to Telomeric DNA Synthesis by Human Telomerase. Journal of Molecular Biology. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, roč. 427, č. 6, s. 1291-1303. ISSN 0022-2836. doi:10.1016/j.jmb.2015.01.008. 2015. URL info
• JANOUŠKOVÁ, Eliška, Ivona NEČASOVÁ a Ctirad HOFR. How human Rap1 prompts more accurate and selective TRF2 recognition of telomeric DNA? In EMBO Workshop Telomeric chromatin and telomere fragility. 2015. info
• JANOUŠKOVÁ, Eliška, Ivona NEČASOVÁ, Jana PAVLOUŠKOVÁ, Michal ZIMMERMANN, Milan HLUCHÝ, María Victoria MARINI PALOMEQUE, Monika NOVÁKOVÁ a Ctirad HOFR. Human Rap1 modulates TRF2 attraction to telomeric DNA. Nucleic Acids Research. Oxford: Oxford University Press, roč. 43, č. 5, s. 2691-2700. ISSN 0305-1048. doi:10.1093/nar/gkv097. 2015. URL info
• NEČASOVÁ, Ivona, Tomáš KLUMPLER, Karel KUBÍČEK a Ctirad HOFR. Revisiting the role of TRF2 basic domain in chromatin maintenance with the most recent structural and functional approaches. In EMBO Workshop Telomeric chromatin and telomere fragility. 2015. info
• JANOUŠKOVÁ, Eliška, Ivona NEČASOVÁ, Michal ZIMMERMANN, Milan HLUCHÝ a Ctirad HOFR. Human Rap1 Induces TRF2 Release from Telomeric DNA. In EMBO Conference Telomeres, Telomerase and disease. 2014. info
• JANOUŠKOVÁ, Eliška, Ivona NEČASOVÁ a Ctirad HOFR. How Rap1 Affects TRF2 Recognition of Telomeres? In XVI. Setkání biochemiků a molekulárních biologů. ISBN 978-80-210-7340-1. 2014. info
• NEČASOVÁ, Ivona, Michal ZIMMERMANN a Ctirad HOFR. How specific is TRF2 binding to telomeric DNA? In EMBO conference Telomeres, telomerase and disease. 2014. info
• BUCHLOVIČ, Marian, Zdeněk KŘÍŽ, Ctirad HOFR a Milan POTÁČEK. New PAH derivatives functionalized by cyclic nitrone framework: Synthetic design, anti-proliferative activity and interaction with DNA. Bioorganic & Medicinal Chemistry. Elsevier, roč. 21, č. 5, s. 1078-1081. ISSN 0968-0896. doi:10.1016/j.bmc.2013.01.004. 2013. URL info
• NEČASOVÁ, Ivona, Michal ZIMMERMANN a Ctirad HOFR. Impact of N-terminal basic domain of protein TRF2 on its DNA binding affinity. In CSHL: Telomeres & Telomerase. 2013. info
• POTÁČEK, Milan, Marian BUCHLOVIČ, Zdeněk KŘÍŽ a Ctirad HOFR. Synthesis, activity and interaction of nitrone functionalized PAH derivatives. 2013. info
• JANOUŠKOVÁ, Eliška a Ctirad HOFR. How does protein Rap1 affect interactions of its binding partner TRF2? In CSHL: Telomeres & Telomerase. 2013. info
• KUBÍČEK, Karel, Hana ČERNÁ, Peter HOLUB, Josef PASULKA, Dominika HROŠŠOVÁ, Frank LOEHR, Ctirad HOFR, Štěpánka VAŇÁČOVÁ a Richard ŠTEFL. Serine phosphorylation and proline isomerization in RNAP II CTD control recruitment of Nrd1. Genes & Development. New York: Cold Spring Harbor Laboratory Press, roč. 26, č. 17, s. 1891-1896. ISSN 0890-9369. doi:10.1101/gad.192781.112. 2012. URL info
• VISACKA, Katarina, Ctirad HOFR, Smaranda WILLCOX, Ivona NEČASOVÁ, Jana PAVLOUŠKOVÁ, Regina SEPSIOVA, Michaela WIMMEROVÁ, Lucia SIMONICOVA, Jozef NOSEK, Jiří FAJKUS, Jack D. GRIFFITH a Lubomir TOMASKA. Synergism of the Two Myb Domains of Tay1 Protein Results in High Affinity Binding to Telomeres*. The Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., roč. 287, č. 38, s. 32206-32215. ISSN 0021-9258. doi:10.1074/jbc.M112.385591. 2012. URL info
• NEČASOVÁ, Ivona, Michal ZIMMERMANN a Ctirad HOFR. N-terminal basic domain of genome guarding telomeric protein TRF2 affects its DNA binding affinity. In EMBO Conference Telomeres and the DNA damage response. 2012. info
• NEČASOVÁ, Ivona, Michal ZIMMERMANN a Ctirad HOFR. Stanovení vlivu bazické domény lidského telomerového proteinu TRF2 na vazbu k DNA. In XII mezioborové setkání mladých biologů, biochemiků a chemiků z Čech a Slovenska. ISSN 0009-2770. 2012. info
• HÓBOR, Fruzsina, Roberto PERGOLI, Karel KUBÍČEK, Dominika HROŠŠOVÁ, Veronika BAČÍKOVÁ, Michal ZIMMERMANN, Josef PASULKA, Ctirad HOFR, Štěpánka VAŇÁČOVÁ a Richard ŠTEFL. Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein. The Journal of Biological Chemistry. Am. Soc. for Biochem. and Mol. Biol., roč. 286, č. 5, s. 3645-3657. ISSN 0021-9258. doi:10.1074/jbc.M110.158774. 2011. info
• ŠTEFL, Richard, Florian OBERSTRASS, Jeniffer HOOD, Muriel JOURDAN, Michal ZIMMERMANN, Lenka SKŘÍŠOVSKÁ, Christophe MARIS, Li PENG, Ctirad HOFR, Ronald EMESON a Frederic ALLAIN. The Solution Structure of the ADAR2 dsRBM-RNA Complex Reveals a Sequence-Specific Readout of the Minor Groove. CELL. UNITED STATES: CELL PRESS, roč. 143, č. 2, s. 225-237. ISSN 0092-8674. 2010. info
• HOFR, Ctirad, Pavla ŠULTESOVÁ, Michal ZIMMERMANN, Iva MOZGOVÁ, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ, Michaela WIMMEROVÁ a Jiří FAJKUS. Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics. BIOCHEMICAL JOURNAL. ENGLAND: PORTLAND PRESS LTD, roč. 419, Neuveden, s. 221-228. ISSN 0264-6021. 2009. info
• ZIMMERMANN, Michal, Ctirad HOFR, Pavla ŠULTESOVÁ, Iva MOZGOVÁ, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ a Jiří FAJKUS. Mechanismus vazby SMH proteinů na telomerickou DNA. In Chemické listy. Praha: Neuveden. 2 s. ISSN 0009-2770. 2009. info
• ZIMMERMANN, Michal, Ctirad HOFR, Pavla ŠULTESOVÁ, Iva MOZGOVÁ, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ a Jiří FAJKUS. Application of fluorescence anisotropy to monitor protein binding to telomeric DNA. In FluoroFest Prague 2009. 2009. info
• MOZGOVÁ, Iva, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ, Ctirad HOFR a Jiří FAJKUS. Functional characterization of domains in AtTRB1, a putative telomere-binding protein in Arabidopsis thaliana. Phytochemistry. England: Pergamon-Elsevier Science Ltd, roč. 69, č. 9, s. 1814-1819. ISSN 0031-9422. 2008. info
• HOFR, Ctirad, Pavla ŠULTESOVÁ, Iva MOZGOVÁ, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ a Jiří FAJKUS. Telomere repeat binding protein variants show distinct kinetics and energetic nature of DNA-protein interaction. In 4th European workshop on: Role of Telomeres and Telomerase in Cancer and Aging. 2008. info
• KUCHAŘ, Milan, Petra SCHRUMPFOVÁ, Iva ŠANTRŮČKOVÁ, Jan PALEČEK, Ctirad HOFR a Jiří FAJKUS. Mapping of interaction domains of putative plant telomere preteins. In Plant DNA Repair & Recombination. 2007. info
• ŠANTRŮČKOVÁ, Iva, Ctirad HOFR, Petra SCHRUMPFOVÁ a Jiří FAJKUS. Characterisation of AtTRB1 binding properties. In Plant DNA Repair & Recombination. 2007. info
• MOZGOVÁ, Iva, Ctirad HOFR, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ a Jiří FAJKUS. Charakterizace vazebných vlastností ATTRB1. In Bulletin České společnosti experimentální biologie rostlin a Fyziologické sekce Slovenské botanické společnosti. OIomouc: Česká společnost experimentální biologie rostlin. s. 66. ISSN 1213-6670. 2007. info
• HOFR, Ctirad. Mikrokalorimetrie bilogicky významných molekul. Československý časopis pro fyziku. Česká republika: Fyzikální ústav AVČR, roč. 56, č. 5, s. 288-292. ISSN 0009-0700. 2006. info
• MITCHELL, Edward, Charles SABIN, Lenka ŠNAJDROVÁ, Martina POKORNÁ, Stephanie PERRET, Catherine GAUTIER, Ctirad HOFR, Nechama GILBOA-GARBER, Jaroslav KOČA, Michaela WIMMEROVÁ a Anne IMBERTY. High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches. Proteins: Structure, Function, and Bioinformatics. Wiley, roč. 58, č. 3, s. 735-746. ISSN 0887-3585. 2005. info
• BRABEC, Viktor a Ctirad HOFR. Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II). BIOPOLYMERS. 111 RIVER ST, HOBOKEN, NJ 07030 USA: JOHN WILEY & SONS INC, roč. 77, č. 4, s. 222-229, 9 s. ISSN 0006-3525. 2005. URL info
• HOFR, Ctirad a Viktor BRABEC. Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., roč. 276, č. 276, s. 9655-9661. ISSN 0021-9258. 2001. info
• HOFR, Ctirad, Nicholas FARRELL a Viktor BRABEC. Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex. Nucleic Acids Research. Oxford, UK: Oxford Press, roč. 29, č. 10, s. 2034-2040. ISSN 0305-1048. 2001. info
• MALINA, Jaroslav, Ctirad HOFR, Lucciana MARESCA, Giovanni NATILE a Viktor BRABEC. DNA interactions of antitumor cisplatin analogs containing enantiomeric amine ligands. Biophysical Journal. New York, USA: Rockefeller University, roč. 2000, č. 78, s. 2008-2021. ISSN 0006-3465. 2000. info
• NOVÁKOVÁ, Olga, Ctirad HOFR a Viktor BRABEC. Modification of natural, double-helical DNA by antitumor cis- and trans-[Cl2(Me2SO4)4Ru] in cell-free media. Biochemical Pharmacology. Oxford, UK: Elsevier, roč. 60, č. 12, s. 1761-1771. ISSN 0006-2952. 2000. info

2022/02/21