C6850 Chromatographic Methods II.

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Tue 10:00–11:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inverse chromatography and hyphenated separation and related analytical techniques;
- asses the possibility of combination of chromatographic and other separation and analytical techniques for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- apply theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capillary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resolution high throughput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018.

C6850 Chromatographic Methods II.

Faculty of Science
spring 2018
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 10:00–11:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inverse chromatography and hyphenated separation and related analytical techniques;
- asses the possibility of combination of chromatographic and other separation and analytical techniques for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- apply theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capillary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resolution high throughput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2017
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Tue 10:00–11:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inverse chromatography and hyphenated separation and related analytical techniques;
- asses the possibility of combination of chromatographic and other separation and analytical techniques for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- apply theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capillary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resolution high throughput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2016
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 11:00–12:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inverse chromatography and hyphenated separation and related analytical techniques;
- asses the possibility of combination of chromatographic and other separation and analytical techniques for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- apply theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capillary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resolution high throughput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2015
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 11:00–12:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inverse chromatography and hyphenated separation and related analytical techniques;
- asses the possibility of combination of chromatographic and other separation and analytical techniques for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- apply theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems.
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 4.- 7. Hybrid electromigration/chromatographic separation methods. Ion progression in an electrical field, the basic equations, concepts, terms and parameters. Principles of electromigration separation, principles of elektrochromatographic methods. Capillary electrophoresis (CE), isotachophoresis (ITP), isoelectric focusing, capillary electrochromatography (CEC), micellar electrokinetic chromatography (MEKC), capillary gel electrophoresis, microemulsion electroinetic chromatography(MEEKC), transient isotachophoresis. Applications. Instrumentation.
  • 8.- 9. Inverse chromatography
  • 10.- 11. Hyphenated techniques in separation analysis.
  • 12. - 13. Trends in chromatography. Multidimensional chromatography. Rapid resolution high throughput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
  • JPC - Journal of Planar Chromatography-Modern TLC, Akadémiai Kiadó, ISSN 0933-4173 (Print) 1789-0993 (Online)
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 11:00–12:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inverse chromatography and hyphenated separation and related analytical techniques;
- asses the possibility of combination of chromatographic and other separation and analytical techniques for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- apply theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capillary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resolution high throughput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2013
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 11:00–12:50 D29/252-RCX1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus (in Czech)
  • 1. Tenkovrstvá chromatografie. Popis experientálního uspořádání. Charakteristické rysy TLC. Fázové systémy TLC. Postupy, kroky planární chromatografie. Vyvíjení chromatogramu, Techniky řízené kapilárními salami, techniky s nuceným tokem mobilní faze. Gradientová eluce v TLC. 2. Instrumentace TLC. Konstrukce a příprava tenké vrstvy, materiály pro stacionární fáze. Vysoce účinná TLC. Dávkování vzorků, Eluční techniky, volba mobilní faze, klasifikace složek mobilní faze, modely Snyder a Nyiredy,. 3. Detekce v TLC. Přímé moetody, derivatizační postupy. Dokumentace. Vyhodnocení TLC 4. Vícerozměrná TLC. Oblasti použití TLC. 5. Hybridní metody, elektrochromatografie, micelární elektrokinetická chromatografie, mikroemulsní elektrokinetická chromatografie, kapilární chirální elektroforéza, gelová elektroforéza. Experimentální podmínky. 6. Pohyb iontu v elektrickém poli, základní rovnice, pojmy a parametry. Elektroosmotický tok. Kapilární elektroforéza (CE), Izotachoforéza, izoelektrická fokusace. Přechodová isotachoforéza. 7. Instrumentace a postupy hybridních technik. Ddávkování vzorků, Detekce 8. Inversní chromatografie. Definice, zařazení v chromatografických metodách 9. Oblasti použití inverzní chroatografie. Výstupy. Studium adsorpčních vlastností pevných materialů, měření adsorpční izotermy –Glueckauf, Gregg/Stock. Adsorpční teplo. 10. Oblasti použití inverzní chroatografie. Mezimolekulové interakce. Porozita chromatografického lože. Studium vlastností imobilizovaných polymer. Humínové kyseliny, biopolymery. Aktivní centra na povrchu pevné faze. Katalytická aktivita. Fázové přeměny. Elekrokinetické studie. 11. Kombinované techniky v separační analýze. Vícerozměrné separace. Spojení LC-GC. On-line extrakce a předseparace, SPE, SPME, SFE, ASE. Spektrální metody pro on-line detekci a identifikaci, GC-MS, LC-MS, GC(LC)-ICP-MS, LC-NMR, LC-FTIR. 12. Trendy v chromatografii. Vícerozměrová chromatografie. Vysoce rychlá chromatografie. Mikroseparace.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2012
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 11:00–12:50 409-stara KAM1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2011
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Wed 9:00–10:50 409-stara KAM1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Hybrid methods of electrophforetic an chromatographic analysis. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of hybrid methods. Eloectrochromatographic methods. Methods of capillary electrochromatography (CEC). Methods of micelar electrochromatography (MEKC, MEEKC).Instrumentation. Experimental properties. Applications. Quantitation.
  • 7.- 8. Inverse chromatography. The use of chromatograpfhic principles for study of phase systems.
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations. Experimental problems in chromatographic methods.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2010
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Wed 9:00–10:50 409-stara KAM1
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Wed 9:00–10:50 kamenice
Prerequisites
Knowledge of principles of chromatograohy corresponding C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to;
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks: 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods
lectures, oral exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2008
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Wed 9:00–10:50 kamenice
Prerequisites
Knowledge of principles of chromatograohy and parameters corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of thin layer chromatography (TLC) capillary electrophoresis (HPCE), capillary electrochromatography (CEC), inverse chromatography and hyphenated techniques.
Syllabus
  • According to number of semester weeks: 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application. 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation. 7.- 8. Inverse chromatography 9.- 10. Hyphenated techniques in separation analysis. 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2007
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
doc. RNDr. Zdeněk Šimek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Tue 9:00–10:50 kamenice
Prerequisites
Knowledge of principles of chromatograohy and parameters corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of thin layer chromatography (TLC) capillary electrophoresis (HPCE), capillary electrochromatography (CEC), inverse chromatography and hyphenated techniques.
Syllabus
  • According to number of semester weeks: 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application. 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation. 7.- 8. Inverse chromatography 9.- 10. Hyphenated techniques in separation analysis. 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2006
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
doc. RNDr. Zdeněk Šimek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Tue 9:00–10:50 kamenice
Prerequisites
Knowledge of principles of chromatograohy and parameters corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of thin layer chromatography (TLC) capillary electrophoresis (HPCE), capillary electrochromatography (CEC), inverse chromatography and hyphenated techniques.
Syllabus
  • According to number of semester weeks: 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application. 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation. 7.- 8. Inverse chromatography 9.- 10. Hyphenated techniques in separation analysis. 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2005
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
doc. RNDr. Zdeněk Šimek, CSc.
Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Tue 11:00–12:50 kamenice
Prerequisites
Knowledge of fundamental chromatographic principles and parameters corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of capillary electrophoresis (HPCE), capillary electrochromatography (CEC), and gas chromatography (HRGC) are discussed in details.
Syllabus
  • I. Capillary electrophoresis and electrochromatography. II.1 Ion progression in electrical field, fundamental equations, terms, and parameters. II.2 Principles of CE techniques and those of capilllary electrochromatography, applications. II.3 Instrumentation. Quantitation. II. Gas chromatography. II.1 Retention characteristics, Gas flow in porous media, van Deemter equation and Golay equation. Retention indexes. II.2 Gas-Liquid Chromatography. Columns, liquid phases and their characterization, supports. II.3 Adsorption gas chromatography. Features, comparison with GLC. Adsorbents, applications. II.4 Capillary columns, packed, WCOT, PLOT, SCOT. Quality evaluation. II.5 Mobile phase, comparison of gases. II.6 Instrumentation, sample injection, detection. GC/MS, GC/AED, and GC/FTIR hyphenation. Multidimensional GC. Parameter programming.
Literature
  • ROOD, D. A Practical Guide to the Care, Maintenance, and Troubleshooting of Capillary Gas Chromatographic Systems. Heidelberg: Huethig Buch Verlag, 1991. Chromatographic Methods. ISBN 3-7785-1898-4. info
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2004
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: SDzk (final examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
doc. RNDr. Zdeněk Šimek, CSc.
Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Mon 13:00–14:50 kamenice
Prerequisites
Knowledge of fundamental chromatographic principles and parameters corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of capillary electrophoresis (HPCE), capillary electrochromatography (CEC), and gas chromatography (HRGC) are discussed in details.
Syllabus
  • I. Capillary electrophoresis and electrochromatography. II.1 Ion progression in electrical field, fundamental equations, terms, and parameters. II.2 Principles of CE techniques and those of capilllary electrochromatography, applications. II.3 Instrumentation. Quantitation. II. Gas chromatography. II.1 Retention characteristics, Gas flow in porous media, van Deemter equation and Golay equation. Retention indexes. II.2 Gas-Liquid Chromatography. Columns, liquid phases and their characterization, supports. II.3 Adsorption gas chromatography. Features, comparison with GLC. Adsorbents, applications. II.4 Capillary columns, packed, WCOT, PLOT, SCOT. Quality evaluation. II.5 Mobile phase, comparison of gases. II.6 Instrumentation, sample injection, detection. GC/MS, GC/AED, and GC/FTIR hyphenation. Multidimensional GC. Parameter programming.
Literature
  • ROOD, D. A Practical Guide to the Care, Maintenance, and Troubleshooting of Capillary Gas Chromatographic Systems. Heidelberg: Huethig Buch Verlag, 1991. Chromatographic Methods. ISBN 3-7785-1898-4. info
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2003
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (assistant)
Guaranteed by
RNDr. Miroslav Ciganek, Ph.D.
RECETOX – Faculty of Science
Prerequisites
Knowledge of fundamental chromatographic principles and parameters corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of capillary electrophoresis (HPCE), capillary electrochromatography (CEC), and gas chromatography (HRGC) are discussed in details.
Syllabus
  • I. Capillary electrophoresis and electrochromatography. II.1 Ion progression in electrical field, fundamental equations, terms, and parameters. II.2 Principles of CE techniques and those of capilllary electrochromatography, applications. II.3 Instrumentation. Quantitation. II. Gas chromatography. II.1 Retention characteristics, Gas flow in porous media, van Deemter equation and Golay equation. Retention indexes. II.2 Gas-Liquid Chromatography. Columns, liquid phases and their characterization, supports. II.3 Adsorption gas chromatography. Features, comparison with GLC. Adsorbents, applications. II.4 Capillary columns, packed, WCOT, PLOT, SCOT. Quality evaluation. II.5 Mobile phase, comparison of gases. II.6 Instrumentation, sample injection, detection. GC/MS, GC/AED, and GC/FTIR hyphenation. Multidimensional GC. Parameter programming.
Literature
  • ROOD, D. A Practical Guide to the Care, Maintenance, and Troubleshooting of Capillary Gas Chromatographic Systems. Heidelberg: Huethig Buch Verlag, 1991. Chromatographic Methods. ISBN 3-7785-1898-4. info
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2002
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Aleš Hrdlička, CSc.
Prerequisites
Knowledge of fundamental chromatographic principles and parameters corresponding to the level of introductory part of C5910 course, Chromatographic methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 19 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of capillary electrophoresis (HPCE), capillary electrochromatography (CEC), and gas chromatography (HRGC) are discussed in details.
Syllabus
  • I. Capillary electrophoresis and electrochromatography. II.1 Ion progression in electrical field, fundamental equations, terms, and parameters. II.2 Principles of CE techniques and those of capilllary electrochromatography, applications. II.3 Instrumentation. Quantitation. II. Gas chromatography. II.1 Retention characteristics, Gas flow in porous media, van Deemter equation and Golay equation. Retention indexes. II.2 Gas-Liquid Chromatography. Columns, liquid phases and their characterization, supports. II.3 Adsorption gas chromatography. Features, comparison with GLC. Adsorbents, applications. II.4 Capillary columns, packed, WCOT, PLOT, SCOT. Quality evaluation. II.5 Mobile phase, comparison of gases. II.6 Instrumentation, sample injection, detection. GC/MS, GC/AED, and GC/FTIR hyphenation. Multidimensional GC. Parameter programming.
Literature
  • ROOD, D. A Practical Guide to the Care, Maintenance, and Troubleshooting of Capillary Gas Chromatographic Systems. Heidelberg: Huethig Buch Verlag, 1991. Chromatographic Methods. ISBN 3-7785-1898-4. info
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2001
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Aleš Hrdlička, CSc.
Prerequisites
Knowledge of fundamental chromatographic principles and parameters corresponding to the level of introductory part of C5910 course, Chromatographic methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 19 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of capillary electrophoresis (HPCE), capillary electrochromatography (CEC), and gas chromatography (HRGC) are discussed in details.
Syllabus
  • I. Capillary electrophoresis and electrochromatography. II.1 Ion progression in electrical field, fundamental equations, terms, and parameters. II.2 Principles of CE techniques and those of capilllary electrochromatography, applications. II.3 Instrumentation. Quantitation. II. Gas chromatography. II.1 Retention characteristics, Gas flow in porous media, van Deemter equation and Golay equation. Retention indexes. II.2 Gas-Liquid Chromatography. Columns, liquid phases and their characterization, supports. II.3 Adsorption gas chromatography. Features, comparison with GLC. Adsorbents, applications. II.4 Capillary columns, packed, WCOT, PLOT, SCOT. Quality evaluation. II.5 Mobile phase, comparison of gases. II.6 Instrumentation, sample injection, detection. GC/MS, GC/AED, and GC/FTIR hyphenation. Multidimensional GC. Parameter programming.
Literature
  • ROOD, D. A Practical Guide to the Care, Maintenance, and Troubleshooting of Capillary Gas Chromatographic Systems. Heidelberg: Huethig Buch Verlag, 1991. Chromatographic Methods. ISBN 3-7785-1898-4. info
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2000
Extent and Intensity
2/0/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
Guaranteed by
doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Aleš Hrdlička, CSc.
Prerequisites
Knowledge of fundamental chromatographic principles and parameters on the level of introductionary part of C5910 course, Chromatographic methods I. CE/MS and GC/MS methods are discussed in C5900 course, Mass spectrometry.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 19 fields of study the course is directly associated with, display
Syllabus
  • I. Capillary electrophoresis and electrochromatography II.1 Ion progression in electrical field. II.2 Principles of CE techniques,applications. Capillary electrochromatography. II.3 Instrumentation. Quantitation II. Gas chromatography II.1 Retention characteristics, Gas flow in porous media. II.2 Gas-Liquid Chromatography: Columns, Interactions for separation. II.3 Adsorption gas chromatography: Features, adsorbents, applications. II.4 Capillary columns: packed, WCOT, PLOT, SCOT. II.5 Mobile phase: Properties of gases. II.6 Instrumentation. GC/MS, GC/AED, and GC/FTIR hyphenation. Multidimensional GC. Parameter programming.
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Prerequisites
Knowledge of principles of chromatograohy corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks:
  • 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
  • Electrokinetic chromatography : theory, instrumentation and applications. Edited by Ute Pyell. Hoboken, N.J.: John Wiley & Sons, 2006, xii, 539. ISBN 0470871024. info
  • LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.

C6850 Chromatographic Methods II.

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Prerequisites
Knowledge of principles of chromatograohy and parameters corresponding to the level of introductory part of C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of thin layer chromatography (TLC) capillary electrophoresis (HPCE), capillary electrochromatography (CEC), inverse chromatography and hyphenated techniques.
Syllabus
  • According to number of semester weeks: 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application. 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation. 7.- 8. Inverse chromatography 9.- 10. Hyphenated techniques in separation analysis. 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.
  • Enrolment Statistics (recent)