PřF:B7722c Metody analýzy mikroorg. II -c

B7722c Speciální metody analýzy mikroorganizmů II. -c

Faculty of Science
Autumn 1999

Extent and Intensity

0/3/0. Type of Completion: z (credit).

Teacher(s)

Guaranteed by

Prerequisites

Laboratory training course is a part of the course B7722.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Syllabus

The first part of laboratory training course is devoted to the work with recombinant plasmid DNA. The second part is devoted to the DNA/DNA hybridisation. The content of laboratory training course is following: Cultivation of bacterial cells carrying recombinant plasmid DNA pUC19. Small scale isolation of recombinant plasmid DNA. Electrotransformation of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM 109. Selection of transformants. Chromatographic purification of recombinant plasmid DNA probe. The labelling of plasmid DNA probe by digoxigenine using nick translation. The preparation of dot blots of bacterial DNA. The carrying out of DNA/DNA hybridisation. Detection of hybrid molecules DNA using antidigoxigenin antibody.

Literature

1. Sambrook J., Fritsch E.F., Maniatis T. (1989) Molecular Cloning. 2 ed., CSHL Press. 2. Darling D.C. and Brickell P.M. (1996) Nucleic Acid Blotting. IRL Press, Oxford.

Assessment methods (in Czech)

Cvičení je ukončeno zápočtem.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.