BX940c Molecular Biology and Genetics - Practice

Faculty of Science
Autumn 1999
Extent and Intensity
0/0/5. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites (in Czech)
NOW ( B7940p Molecular biology )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Syllabus
  • Isolation of plasmid DNA; Determination of purity and concentration of DNA; Digestion of DNA by restriction endonucleases; Horizontal slab gel electrophoresis of DNA in agarose gels; Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids); Ultracentrifugation in sacharose gradient. Analysis of genomic bacterial DNA: identification of specific genes, characterization of bacterial strains by DNA fingerprinting; Pulsed-field gel electrophoresis of bacterial genomic DNA (preparation of samples, digestion with restriction endonuclease SmaI, PFGE); DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection); PCR (polymerase chain reaction), RAPD-PCR; DNA sequencing.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn1999/BX940c