B7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2000
Extent and Intensity
3/0/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites (in Czech)
( B6390 Molecular Methods || B6400 Methods of molecular biology ) && NOW( B7311 Mol. biol. of procar.-practice )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 1999, Autumn 2001.
  • Enrolment Statistics (Autumn 2000, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2000/B7120