PřF:B7311 Mol. biol. of procar.-practice - Course Information

B7311 Molecular Biology of Procaryotes - Laboratory Course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.

Prerequisites (in Czech)

NOW( B7120 Molecular biology of prokaryotes )

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24

fields of study / plans the course is directly associated with

• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

Pulsed-field gel electrophoresis of bacterial genomic DNA (preparation of samples, digestion with restriction endonuclease SmaI, PFGE). Isolation of spontaneous mutants of Staphylococcus aureus resistant to antibiotics on gradient agar plates. Fluctuation test and estimation of mutation rate. Conjugation of Escherichia coli. Replica plating. Isolation of auxotroph mutants of Staphylococcus aureus.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.

• Enrolment Statistics (Autumn 2000, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2000/B7311