B7312 Practical course of molecular biology of eukaryotes

Faculty of Science
Autumn 2001
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites (in Czech)
NOW( B7090 Eukaryotic cells )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Principles of cultivation of eukaryotic cells in vitro. Transfer of DNA into eukaryotic cells: transient transfection of fibroblasts using calcium phosphate precipitation and electroporation. Deteremination of exogenous DNA expression in transfected cells: SDS-PAGE of proteins and western blotting, measuring of enzyme activities of gene products (luciferase and beta-galactosidase assays).
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Autumn 1999, Autumn 2000.
  • Enrolment Statistics (recent)
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