IB024 Mapping the Arabidopsis core cell cycle machinery by targeted interactomics

Faculty of Science
Autumn 2010
Extent and Intensity
0/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. Geert de Jaeger (lecturer)
Guaranteed by
doc. RNDr. Jan Hejátko, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The mapping of protein interactions is a popular method for gene discovery, functional gene analysis or the study of gene networks. Yeast two-hybrid or protein complementation assays are used to map binary interactions, but the emergence of powerful, ultrasensitive high-throughput mass spectrometry (MS), together with the availability of comprehensive protein sequence repertoires, has favored the development of methods relying on in situ affinity purification of protein complexes. Especially, the tandem affinity purification (TAP) approach, based on the expression of a bait protein fused to a double affinity tag, has proven to be of great value. We developed a high throughput TAP technology platform for complex isolation from Arabidopsis cell suspension cultures (1,2) and applied it to map the core complex machinery at the heart of the Arabidopsis cell cycle control. The resulting network offers for the first time a comprehensive view on CDK/cyclin complexes of a higher plant and contains over 100 new candidate cell cycle proteins. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Furthermore, we could demonstrate that plants have evolved a combinatorial toolkit comprising at least a hundred different CDK/cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants.
Language of instruction
English
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught only once.
The course is taught: in blocks.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2011 - acreditation.
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