C9033 Classical & Molecular Cytogenetics – Selected Topics

Faculty of Science
Autumn 2013
Extent and Intensity
0/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Prof. dr hab. Ingo Schubert (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: prof. Mgr. Martin Lysák, Ph.D., DSc.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15, only registered with preference (fields directly associated with the programme): 0/15
Syllabus (in Czech)
  • Program: Introduction and microscopic exercises to selected topics (see below).
  • Tracing and interpretation of chromosome rearrangements with evolutionary consequences.
  • The focus will be on technologies which are complementing and needed for correct interpretation of genomic, molecular and cell genetic research approaches.
  • Seminar on ‘how to get a scientific paper’
  • 1. Aims of Cytogenetics; mitotic cell cycle; chromosome structure & morphology
  • 2. Meiosis (interpretation of normal and aberrant stages)
  • 3. Chromosome mutagenesis (incl. apoptosis in interspecific hybrids and gametocidal effects)
  • 4. Karyotype analysis by chromosomal length differentiation via banding techniques (G, R, C, T, N, AgNO3, RE, Q…, mobile NORs, nucleolar dominance)
  • 5. Differential labeling of chromosomes by base analogues (cell cycle, replication patterns; SCE)
  • 6. Mechanisms of karyotype evolution (ploidy level; primary & secondary rearrangements; chromosome number alterations, incl. fusion/fission and B-B-F-cycles; too long/too short chromosomes)
  • 7. In situ hybridization & immunostaining (FISH, GISH, PRINS, Painting & applications)
  • 8. Microdissection, flow-sorting & applications
  • 9. Telomeres & Centromeres - prerequisites for eukaryotic chromosomes
  • 10. Heterochromatin & Euchromatin, features and marks
  • 11. Interphase chromosome organization (homologous pairing; sister chromatid cohesion, ‘Rabl’ orientation - mirror image orientation; meristematic vs. differentiated tissues; evolutionary conservation)
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course is taught only once.
Information on the per-term frequency of the course: 23..-27.9.2013.
The course is taught: in blocks.
General note: www: http://www.ipk-gatersleben.de/Internet/Forschung/CytogenetikGenomanalyse/Karyotypevolution.
Information on the extent and intensity of the course: 1D.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2013/C9033