PřF:Bi5130c Practical trainin in aDNA - Course Information

Bi5130c Introduction to human aDNA studies practical training

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. et Mgr. Kristýna Brzobohatá, Ph.D. (seminar tutor)

Guaranteed by

Mgr. et Mgr. Kristýna Brzobohatá, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. et Mgr. Kristýna Brzobohatá, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi5130c/01: Thu 13:00–14:50 D36/347, K. Brzobohatá
Bi5130c/02: Thu 15:00–16:50 D36/347, K. Brzobohatá

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16

fields of study / plans the course is directly associated with

• Bioanalytical Laboratory Diagnostics in Medicine - Medical Genetics and Molecular Diagnostics (programme PřF, N-LGM)
• Medical Genetics and Molecular Diagnostics (programme PřF, N-BI)
• Medical Genetics and Molecular Diagnostics (programme PřF, B-BI)
• Medical Genetics and Molecular Diagnostics (programme PřF, B-LGM)
• Molecular Biology and Genetics (programme PřF, B-EMB)
• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• Molecular Biology and Genetics (programme PřF, N-MBG)
• Special Biology (programme PřF, B-EXB)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Antropobiology and Antropogenetics)

Course objectives

The aim of the course is practical skills of genetic analysis of human ancient DNA - selection of a suitable sample, treatment and purification of selected tissue, DNA isolation, DNA amplification, DNA sequencing and evaluation of results. Students will also learn to work under DNA-free conditions.

Learning outcomes

After course, the student will be able to: - select a suitable part of human skeleton for analysis of aDNA and separate methods by appropriate methods - clean and treat the selected tissue to minimize the risk of recent DNA contamination - lysate bone tissue and purify aDNA - select the amplification protocol for a given target sequence (primer design, PCR modification ...) - Visualize the PCR products via ELFO and enzymatically purify them for sequencing - evaluate the acquired sequence and interpret it in historical contexts

Syllabus

• Tissue collection, sample cleaning, bone tissue lysis Isolation of a DNA Amplification of HVR I mtDNA ELFO, sample preparation for sequencing Evaluation of results

Literature

• HUMMEL, Susanne. Ancient DNA typing : methods, strategies and applications. Berlin: Springer, 2010, xii, 298. ISBN 9783642077050. info

Teaching methods

Laboratory practice

Assessment methods

Protocol indicating: - the material and methods used - photos of analyzed samples, determination of their preservation - a slide of ELFO PCR products, an evaluation of the success of the reaction - the electropherotram of the sequences obtained - a summary of the sequence obtained - putting into a historical context (e.g., mtDNA)

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

• Enrolment Statistics (Autumn 2019, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2019/Bi5130c