Bi9393c Analytical cytometry - practical course

Faculty of Science
Autumn 2020
Extent and Intensity
0/1/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Stanislav Drápela, Ph.D. (seminar tutor), Mgr. Radek Fedr (deputy)
Mgr. Markéta Hrušková (seminar tutor)
Ing. Michaela Chorvátová (seminar tutor)
Mgr. Barbora Kvokačková, Ph.D. (seminar tutor)
Mgr. Ondřej Vacek (seminar tutor)
Guaranteed by
Mgr. Karel Souček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 17. 5. 8:00–12:00 BFU, 13:00–17:00 BFU, Tue 18. 5. 8:00–12:00 BFU, 13:00–17:00 BFU, Wed 19. 5. 8:00–12:00 BFU, 13:00–17:00 BFU, Thu 20. 5. 8:00–12:00 BFU, 13:00–17:00 BFU
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16, only registered with preference (fields directly associated with the programme): 0/16
fields of study / plans the course is directly associated with
Course objectives
Practical training in basic methods of flow cytometry and high speed sorting.
Learning outcomes
The student will learn basic methods of cell analysis using flow cytometry including sample preparation acquisition and data analysis.
Syllabus
  • Basic training in operation of routine flow cytometer FACSCalibur. Setup, calibration, quality control. Basic training in operation of high speed sorter. Setup, calibration, quality control. Cell cycle analysis. Immunophenotype analysis. GFP expression analysis and sorting. Data analysis - software FlowJo.
Literature
  • Current Protocols in Cytometry, Print ISSN: 1934-9297 Online ISSN: 1934-9300
Teaching methods
practical training
Assessment methods
Evaluation of protocols.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2020/Bi9393c