S5015 Light microscopy methods in biology

Faculty of Science
autumn 2021
Extent and Intensity
1/0/0. 1 credit(s) (plus 1 for the colloquium). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Milan Ešner, Ph.D. (lecturer)
Wojciech Krzysztof Jesionek, M.Sc., Ph.D. (lecturer)
Mgr. Petra Kučerová, Ph.D. (lecturer)
Mgr. Jakub Pospíšil, Ph.D. (lecturer)
Guaranteed by
Mgr. Milan Ešner, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: Jitka Kvěchová
Supplier department: Central European Institute of Technology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives (in Czech)
The main goal of this course is to to acquaint students with the principles of light microscopy. Main imaging modalities used in life sciences research will be explained. Theoretical part will complemented with practicals.
It is recommended to complete the course in combination with "S5015c Light microscopy methods in biology - practical course".
Learning outcomes (in Czech)
Students will be able to explain the basic principles and uses of light microscopy in life sciences reasearch.
It is recommended to complete the course in combination with "S5015c Light microscopy methods in biology - practical course".
Syllabus (in Czech)
  • 4 x 90 minutes lectures - possible attend online via MS Teams or watch the recordings of the lectures (available as part of the study materials in IS MU):
  • 1) 30.9.2021, 9:00 - History of microscopy, fundamentals of light microscopy. Contrasting techniques in transmitted light microscopy – brightfield, darkfield, phase contrast, DIC. Widefield fluorescence microscopy - deconvolution, point spread function, point spread function measurements, description. Detectors in widefield microscopy, filters, light sources.
  • 2) 14.10.2021, 9:00 - Confocal microscopy - principles, types of confocal microscopes – scanning, spinning disc, channel vs spectral imaging, linear unmixing. Lasers, detectors, beam splitters, optical sections. Lightsheet microscopy.
  • 3) 25.10.2021, 9:00 - Imaging below diffraction limits – SIM, SMLM, STED.
  • 4) 4.11.2021, 9:00 - Basics of Image analysis – image types, pixels, voxels, basic filters, image processing, metadata, deconvolution, 3D visualization, objects detection, quantification.
  • It is recommended to complete the course in combination with "S5015c Light microscopy methods in biology - practical course".
Teaching methods (in Czech)
online lectures (e-learning)
Assessment methods (in Czech)
colloqium – discussion in a small group
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
Note related to how often the course is taught: 4 x 90 minutes lectures per semestr.
Information on the extent and intensity of the course: 4 x 90 minutes lectures per semestr.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2021/S5015