S5015c Light microscopy methods in biology – practical course

Faculty of Science
autumn 2021
Extent and Intensity
0/0/0. 1 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
Mgr. Milan Ešner, Ph.D. (lecturer)
Wojciech Krzysztof Jesionek, M.Sc., Ph.D. (lecturer)
Mgr. Petra Kučerová, Ph.D. (lecturer)
Mgr. Jakub Pospíšil, Ph.D. (lecturer)
Guaranteed by
Mgr. Milan Ešner, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: MVDr. Jana Tšponová, Ph.D.
Supplier department: Central European Institute of Technology
Prerequisites (in Czech)
The practical part can be done only if the student is enrolled in the online theoretical part - S5015 Light microscopy methods in biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives (in Czech)
The main goal of this practical course is to acquaint students with the principles of light microscopy. Main imaging modalities used in life sciences research will be explained and showed in practice.
Learning outcomes (in Czech)
Students will be able to explain the basic principles and uses of light microscopy in life sciences reasearch. Students will be able to explain how to prepare samples, use microscopes and analyze data.
Syllabus (in Czech)
  • practical 8 hours in two blocks (two days = 2x8 hours):
  • Day 1: Transmitted light techniques – brightfield, phase contrast, DIC, darkfield. Sample preparation for fluorescence microscopy. Image acquisition using widefield, confocal and structured illumination microscopy (Apotome, lattice SIM).
  • Day 2: Single molecule localisation microscopy – sample preparation and image acquisition. Image analysis part – image processing, deconvolution, single molecule localisation data analysis, object segmentation and quantification.
  • The practical part can be done only if the student is enrolled in the online theoretical part - S5015 Light microscopy methods in biology.
Teaching methods (in Czech)
Students perform laboratory experiments according to curriculum. Study materials are available in the IS MU.
Assessment methods (in Czech)
active involvement in practical tasks and discussions
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course is taught: in blocks.
Note related to how often the course is taught: practical 8 hours in two blocks (two days = 2x8 hours).
Information on the extent and intensity of the course: practical 8 hours in two blocks (two days = 2x8 hours) - 7.1. + 10.1.2022, C02, room 1.15, 1.13a, 9:00 - 17:00.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2021/S5015c