Bi7035c Special Virological Techniques - practical course

Faculty of Science
Autumn 2024
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
prof. RNDr. Daniel Růžek, Ph.D. (lecturer)
doc. RNDr. Luděk Eyer, Ph.D. (lecturer)
RNDr. Jiří Salát, Ph.D. (lecturer)
Mgr. Jiří Holoubek, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Daniel Růžek, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 11:00–12:50 D36/213
Prerequisites
Bi5420 General virology && NOW( Bi7035 Special Virological Techniques )
The subject is designated for virology students.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 6/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
The student will be able to understand and explain the basic virological techniques, use the information about the methods for their application in practise, make decisions based on the interpretation of the results, apply the practical knowledge in the laboratory, understand the principles of the methods, have basic experience in handling infectious material and under aseptic conditions, be involved in laboratory practise.
Learning outcomes
The student will have a basic experience in handling infectious materiál and under aspetic conditions, will know how to work with cell cultures and virus culture in vitro and in vivo, will understand the basic as well as advanced virological methods, will be able to interpret the results of virological analyses.
Syllabus
  • The practical course takes place in the form of block teaching due to the time-consuming nature of the individual methods. The student will become familiar with the following tasks: Virus growth curve; Plaque titration; Interference test; Hemadsorption; Purification of viruses; Inoculation of chicken embryos; Infection and dissection of the laboratory mouse; Virus-neutralization test; Hemagglutination and inhibition of hemagglutination.
Literature
    recommended literature
  • MISTRÍKOVÁ, Jela. Základné laboratórne techniky používané vo virológii. Univerzita Komenského, 2000. ISBN 978-80-223-1483-1. info
Teaching methods
Laboratory practical experiments, demonstration of selected basic as well as advanced virological methods.
Assessment methods
The participation in the practical course is mandatory. At the end, there will be a final test and students will present their laboratory protocols.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2024/Bi7035c