E0210 Chromatographic Methods

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Mgr. Jiří Kohoutek, Ph.D. (lecturer)
Mgr. Petr Kukučka, Ph.D. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Zdeněk Šimek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Mon 11:00–12:50 D29/252-RCX1
Prerequisites
The course requires a basic knowledge of analytical chemistry, the insight into instrumental separation methods is an advantage.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to explain the basic theory of chromatographic separation and apply it to individual types of chromatographic methods. Detailed description of the main chromatographic systems, principles of sample injecting and detection techniques. The course describes trends in the development of chromatographic techniques. Students will acquire the expertise needed to select a suitable method for a given analysis and to optimize the experimental conditions of chromatographic methods.
Learning outcomes
Students completing the course will be able to:

- understand basic principle of the most commonly used; chromatographic methods and some other analytical separation techniques;

- know important practical and technical aspects of modern chromatography instrumentation applicable and highly relevant to scientific work;

- propose a protocol and technical implementation of chromatographic methods in relation to the type of analysis and the physico-chemical properties of the analyte;

- explain generic separation theory of chromatographic separation and underlying mechanisms and select suitable chromatographic system, stationary phase and separation mode to obtain high-quality scientific data;

- apply chromatographic theory to characterize analytical systems, surface and material of stationary phase, and supposed interaction of the analyte within the system;

- evaluate and interpret results of chromatographic analysis;

- assess the practical relevance of the combination of chromatographic techniques with different types of detection techniques, in particular with spectroscopic and spectrometric techniques;

- overview current trends in the instrumentation, stationary phases and method development - utilize chromatographs for qualitative and quantitative determination of various analytes in biological matrices;

- select and optimize experimental conditions of separation techniques suitable to increase selectivity, separation efficiency, number of identifications and optimal quantification limits for the most sensitive analysis
Syllabus
  • 1. Basic terms of chromatographic separation, chemical equilibrium, retention parameters, chromatogram
  • 2. Chromatographic resolution, separation efficiency, elution zone, theoretical lplates. Knox and van Deemter equation.
  • 3. Gas chromatography. Mobile and stationary phases for GC, comparison of gas properties.
  • 4. GC instrumentation, sample injecting, types of detectors and the principle of detection.
  • 5. Liquid chromatography (LC), column and planar chromatography, separation conditions.
  • 6. Liquid chromatography (LC), chromatographic columns, types of stationary phases, chemical nature and chromatographic properties of stationary phases.
  • 7. Liquid chromatography (LC) Mobile phase, classification of solvents, buffers and mobile phase additives, mobile phase for gradient elution, optimization procedures, elution modes.
  • 8. Liquid chromatography (LC): normal (NP), reverse (RP), ion-exchange (IEC), supercritical fluid (SEC), gel (GPC), exclusion (SEC).
  • 9. Instrumentation for high performance (HPLC) and ultra-performance (UHPLC) liquid chromatography, mobile phase transport, sample injecting, types of detectors and the principle of detection.
  • 10. Trends in the development of chromatographic methods, development of stationary phases
  • 11. Examples of applications of chromatographic methods
  • 12. Inverse chromatography
  • 13. Hiphenated techniques
Literature
    recommended literature
  • GIDDINGS, John Calvin. Unified Separation Science. Wiley, 1991, 352 pp. ISBN 0-471-52089-6. info
  • ROBARDS, Kevin, Paul R. HADDAD and Peter E. JACKSON. Principles and practice of modern chromatographic methods. London: Academic Press, 1994, x, 495. ISBN 0125895704. info
  • NOVÁKOVÁ, Lucie and Michal DOUŠA. Moderní HPLC separace v teorii a praxi. 1. vyd. [Klatovy]: Michal Douša, 2013, 299 s. ISBN 9788026042433. info
  • NOVÁKOVÁ, Lucie and Michal DOUŠA. Moderní HPLC separace v teorii a praxi. 1. vyd. [Klatovy]: Michal Douša, 2013, 235 s. ISBN 9788026042440. info
Teaching methods
The teaching is performed in the form of a lecture with a Powerpoint presentation. Students will receive printed copies of the presentations before each lecture for entering their own notes and questions. The comprehensibility of difficult parts is checked interactively.
Assessment methods
Attendance at the lecture is not mandatory, but recommended for easy fluency and understanding of the material. During the lesson, the emphasis is on interaction with students and discussion on the topic of the subject already covered is encouraged. The acquired knowledge is verified by an oral exam.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Teacher's information
https://is.muni.cz/auth/predmety/predmet?pvysl=30297613
The course is also listed under the following terms Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
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