PřF:S1004 Structure of biomolecules - Course Information
S1004 Methods for structural characterization of biomolecules
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- Mgr. Josef Houser, Ph.D. (lecturer)
Mgr. Filip Melicher (lecturer)
MVDr. Eva Paulenová, Ph.D. (lecturer)
prof. RNDr. Michaela Wimmerová, Ph.D. (lecturer) - Guaranteed by
- Mgr. Josef Houser, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science - Timetable
- Mon 13. 1. 9:00–16:50 C04/211, Tue 14. 1. 9:00–16:50 C04/211, Wed 15. 1. 9:00–16:50 C04/211, Thu 16. 1. 9:00–16:50 C04/211, Fri 17. 1. 9:00–16:50 C04/118, 9:00–16:50 C04/211, Mon 27. 1. 9:00–16:50 C04/211, Tue 28. 1. 9:00–16:50 C04/211, Wed 29. 1. 9:00–16:50 C04/211, Thu 30. 1. 9:00–16:50 C04/211, Fri 31. 1. 9:00–16:50 C04/211
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of the course students should be able to:
characterize protein sample for structural studies
understand and explain principles of crystal structure determination of proteins
solve X-ray protein structure
interpret electron density maps, analyze and validate pdb coordinates - Learning outcomes
- At the end of the course students should be able to:
characterize protein sample for structural studies
understand and explain principles of crystal structure determination of proteins
solve X-ray protein structure
interpret electron density maps, analyze and validate pdb coordinates - Syllabus
- 1. Biomolecular sample, characteristics, sample requirements 2. Sample quality analysis – stability, homogeneity 3. Structural methods for analysis of biomolecules, complementarity of techniques 4. Biomacromolecular crystals, crystallization techniques, crystallization experiment 5. Crystal properties, space group and symmetry, assymetric unit 6. Diffraction experiment, diffraciton images processing, methods for phasing, electron density 7. Structural model, density maps, model accuracy, validation, structural databases 8. Optimization of structural experiment 9. Laboratory practicals – characterization of protein sample and crystallization experiments
- Literature
- recommended literature
- PECORA R. Dynamic Light Scattering: Applications of Photon Correlation Spectroscopy, Springer, 1985
- Bergfors, T. M. Protein Crystallization: Second Edition, Oxford University Press, 2009.
- Fundamentals of crystallography. Edited by Carmelo Giacovazzo. 2nd ed. Oxford: Oxford University Press, 2002, xix, 825 s. ISBN 0-19-850957-X. info
- Circular dichroism and the conformational analysis of biomolecules. Edited by Gerald D. Fasman. New York: Plenum Press, 1996, ix, 738 s. ISBN 0-306-45142-5. info
- Teaching methods
- Intensive course covering lectures and class discussion
- Assessment methods
- in-term assessment, final written test
- Language of instruction
- English
- Further comments (probably available only in Czech)
- The course can also be completed outside the examination period.
The course is taught annually. - Teacher's information
- Course is being taught as a block of lectures, discussions and laboratory exercises at the end of semester - in the examination period.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2024/S1004