PřF:B6120 Plant Tissue and Organ Culture - Course Information

B6120 Plant Cell, Tissue, and Organ Culture

Extent and Intensity

2/2/0. 4 credit(s). Type of Completion: zk (examination).

Teacher(s)

RNDr. Jaroslava Dubová, CSc. (lecturer)

Guaranteed by

RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová

Prerequisites (in Czech)

B4060 Plant physiology && B6560 Plant physiology

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Plant Physiology)
• General Biology (programme PřF, N-BI, specialization Plant Physiology)

Course objectives

Historical Background, Conditions of in vitro Culture. In vitro Vegetative Multiplication, Types of Regeneration, Thermotherapy. Callus Culture. Suspension Cell Culture, Direct and Indirect Somatic Embryogenesis. Test-tube Pollination, Fertilisation in vitro. Embryo Culture. Androgenesis (Anther Culture, Microspore and Pollen Culture), Gynogenesis (Ovary and Ovule Culture), Dihaploidization. Protoplast Culture, Isolation and Fusion of Protoplasts, Somatic Hybridisation. Direct and Indirect Methods of Transformation. Cryopreservation.

Literature

• Cell and tissue culture :Laboratory procedures. Insertion instructions. Edited by A. Doyle - J. B. Griffiths - D. G. Newell. Chichester: John Wiley & Sons, 1993, Volné lis. ISBN 0-471-92852-6. info
• ONDŘEJ, Miloš. Genové inženýrství kulturních rostlin. 1. vyd. Praha: Academia, 1992, 232 s. ISBN 80-200-0310-X. info

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

• Enrolment Statistics (Spring 2001, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2001/B6120