Bi0250c Diagnostic methods in parasitology - practice

Faculty of Science
Spring 2011
Extent and Intensity
0/2. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Oleg Ditrich, CSc. (lecturer), prof. RNDr. Milan Gelnar, CSc. (deputy)
RNDr. Božena Koubková, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Milan Gelnar, CSc.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Milan Gelnar, CSc.
Prerequisites (in Czech)
SOUHLAS
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Main objectives can be summmarized as follow: to gain basic information on parasites of humans (protozoans, helmiths and arthropods) and on diseases transmitted by these parasites. The course is not elementary one and it is organized according to practical approaches, it means not according to parasite system. Big attention is devoted to autochtonic parasites, however tropical ones represent also very significant part of the course.
Syllabus
  • 1: Procedures for detecting blood parasites. Thick and thin blood films, Pappenhaim staining. 2: Diagnosis of trichomoniasis, cultivation and demonstration of parasites. 3: Cultivation of amphizoic amoebae. Serlogical methods: IFAT 4: Grocott silver staining. Serological methods: ELISA, imunoblot 5: Isolation of DNA from oocyst of Cryptosporidium. PCR, identification. 6.: Methods of cultivation of microsporidia. Inverted microscopy, fluorescence microscopy. 7.: Histological techniques: fixation and preparation of parasitological material for histological investigation. 8.: Histological techniques: HE staining 9.:Parasite species identification - basic methods 10.: Documentation of parasitological material, drawing, microphotography. 11.: Parasite cultures in vivo: Schistosoma mansoni - experimental infection 12.: Biopsy material. Cultivation of dermatophyta. 13. Parasitological laboratory - visit
Literature
  • Garcia L.S., Bruckner D.A., 1993: Diagnostic Medical Parasitology. II. vydání, ASM Washington, 1 - 764
  • Doporučená literatura viz osnova předmětu
  • Jírovec O. a kol., 1977: Parasitologie pro lékaře, III. vydání, AVICENUM Praha, 1 - 798
  • Ash L.R., Orihel T.C., 1990: Atlas of human Parasitology. III. vydání, ASCP Chicago, 1 - 262 Orihel T.C., Ash L.R., 1995: Parasites in human tissues. ASCP Chicago, 1 - 386 Schmidt G.D., Roberts L. Foundations of Parasitology. Fifth Edition. Wm. C. Brown
Assessment methods
oral exam
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught once in two years.
Information on the per-term frequency of the course: Kurz otevírán v JS lichých let (ak. rok 2004/5).
The course is taught: in blocks.
General note: Povinné pro parazitology.
Information on course enrolment limitations: Minimálně tři studenti
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2007, Spring 2009, spring 2012 - acreditation.
  • Enrolment Statistics (recent)
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