S2006 Fluorescence methods in life sciences – A journey from a molecule to cells

Faculty of Science
Spring 2015
Extent and Intensity
2/0. 2 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Ctirad Hofr, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable
Mon 11. 5. 9:00–11:50 E35/2S087, Tue 12. 5. 9:00–11:50 E35/2S087, Wed 13. 5. 9:00–11:50 E35/2S087, Thu 14. 5. 9:00–11:50 E35/2S087, Fri 15. 5. 9:00–11:50 E35/2S087
Course Enrolment Limitations
The course is offered to students of any study field.
Syllabus
  • Definition and characteristics of absorption, fluorescence and phosphorescence Instrumentation - spectrophotometer, spectrofluorometer Steady-state fluorescence Time-resolved fluorescence Fluorescence anisotropy and its change after interaction of macromolecules Absorption spectroscopy in biological analysis of molecules Fluorescence quenching, resonance energy transfer - application for studies of structural changes of biomolecules Intrinsic fluorescence of biologically important molecules Extrinsic fluorescence – fluorescent labels, probes, and indicators Fluorescence labeling of DNA and proteins Fluorescence microscopy Analytical applications of fluorescence for concentration determination Examples of fluorescence application in biology
Literature
  • LAKOWICZ, Joseph R. Principles of fluorescence spectroscopy. 3rd ed. New York: Springer, 2006, xxvi, 954. ISBN 0387312781. info
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms spring 2012 - acreditation, Spring 2016, Spring 2017, spring 2018, Spring 2019.
  • Enrolment Statistics (Spring 2015, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2015/S2006