Bi8870c Mechanisms of cell death - practical lesson

Faculty of Science
Spring 2017
Extent and Intensity
0/1. 1 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Hyršlová Vaculová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
The practical course accompanies the lecture Mechanisms of cell death, significance, methods. Within the two six-hour-blocks students will have an opportunity to practically learn and perform several selected modern methods of apoptosis detection using flow cytometry and microscopy. Due o the complex innovation of this course in 2015, several new flow cytometry-based methods of the cell death detection have been introduced and optimized for the effective realization within the practical courses. Specifically, students will have an opportunity to learn multiparametric analysis of the activation of selected specific molecules (active caspase-3) and events (changes in plasma membrane summetry, phosphatidyl serine translocation) involved in cell death regulation. Moreover, these will be studied simultaneously in combination with analysis of other important cellular processes such as cell death progression (total DNA content, propidium iodide staining), DNA damage (gamma-H2AX phosphorylation) and cell viability (specific probes). Therefore, the students will benifit not only from learning to detect the „classical“ cell death parameters, but also to study them in context of other crucial processes affecting the final cell fate. The innovation of the practical course represents important improvement in complex study of the cell death and correct interpretation of the data obtained. Finally, this will enable students to get better and more relevant view of the complex cell death regulation. They will be provided with information how to evaluate the results obtained, interprete the data, and get practical recommendations about the application of individual methods, their advantages, disadvantages, and limits.
Syllabus
  • selected apoptosis detection methods using - flow cytometry - microscopy
Literature
  • Apoptosis methods and protocols. Edited by Hugh J. M. Brady. Totowa, N.J.: Humana Press, 2004, xi, 304. ISBN 0896038734. info
  • LOCKSHIN, R. A. and Zahra ZAKERI. When cells die 2 : a comprehensive evaluation of apoptosis and programmed cell death. 1st ed. Hoboken: Wiley-Liss, 2004, xi, 549. ISBN 0471219479. info
Teaching methods
laboratory practical course, practical performance of selected methods of apoptosis detection
Assessment methods
Attendance of the whole practical course and practical realization of the methods of the course.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
The course is also listed under the following terms spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2017, recent)
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