PřF:S2006 Applied fluorescence - Course Information

S2006 Fluorescence methods in life sciences - a journey from molecules to cells

Faculty of Science
Spring 2017

Extent and Intensity

2/0. 2 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)

Guaranteed by

Timetable

Mon 20. 2. to Mon 22. 5. Tue 13:00–14:50 C02/211

Course Enrolment Limitations

The course is offered to students of any study field.

Syllabus

Definition and characteristics of absorption, fluorescence and phosphorescence Instrumentation - spectrophotometer, spectrofluorometer Steady-state fluorescence Time-resolved fluorescence Fluorescence anisotropy and its change after interaction of macromolecules Absorption spectroscopy in biological analysis of molecules Fluorescence quenching, resonance energy transfer - application for studies of structural changes of biomolecules Intrinsic fluorescence of biologically important molecules Extrinsic fluorescence – fluorescent labels, probes, and indicators Fluorescence labeling of DNA and proteins Fluorescence microscopy Analytical applications of fluorescence for concentration determination Examples of fluorescence application in biology

Literature

LAKOWICZ, Joseph R. Principles of fluorescence spectroscopy. 3rd ed. New York: Springer, 2006, xxvi, 954. ISBN 0387312781. info

Language of instruction

English

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Teacher's information