IC162 ICP-MS Course

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
Dr. Norbert Jakubowski (lecturer), prof. RNDr. Viktor Kanický, DrSc. (deputy)
Guaranteed by
prof. RNDr. Viktor Kanický, DrSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Prerequisites
Basic knowledge of atomic spectroscopy.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Introduction to mass spectrometry in inductively coupled plasma, theory, instrumentation, research and applications.
Learning outcomes
Students will acquire knowledge about the principle of mass spectrometry, ionization source based on inductively coupled plasma discharge, various designs of mass spectrometers, measurement of signals and causes and corrections of interference, analytical properties of the method, applications of ICP-MS in conjunction with electrothermal atomization, separation methods, with applications for measuring isotope ratios, with application in life sciences, with laser ablation and imaging, in the analysis of polymers, ceramic materials and geological samples by laser ablation, use in nanoparticle analysis, single particles and single cells. Students will acquire information about current state of research and applications and about current trends in ICP-MS. The student will learn to orientate in the field of element analysis using mass spectrometry, using ICP, laser ablation and hyphenated methods.
Syllabus
  • ICP-MS Course Lecturer: Norbert Jakubowski 10 days á 120 min (20 hours) Starting: April 2019, for 4 weeks 25.4.2019 Thursday 14.00-15.00 26.4.2019 Friday 9.00-11.00 30.4.2019 Tuesday 10.00-12.00 14.00-16.00 3.5.2019 Friday 9.00-11.00 7.5.2019 Tuesday 10.00-12.00 14.00-16.00 10.5.2019 Friday 9.00-11.00 14.5.2019 Tuesday 10.00-12.00 14.00-16.00 17.5.2019 Friday 9.00-11.00 Outline: Lecture 1: Introduction to and fundamentals of ICP-MS Introduction History Fundamentals Sample introduction Lecture 2: Hardware of ICP-MS and instrumental concepts ICP Interface Mass Spectrometer Detector Computer and data handling Lecture 3: Spectral interferences in ICP-MS Spectral and non-spectral interferences Instrumental concepts Quadrupole devices Collision and Reaction Cell devices Sector Field Instruments Single Collector Multi-collector Time of flight instruments Ion traps MS/MS Instruments Operation Scan modes Time resolved measurements FIA Single particle mode Single cell mode Calibration strategies Lecture 4: Analytical figures of merit (definitions) and application 1: ETV-ICP-MS Analytical Figures of Merit (accuracy and precision), Statistics, Method validation, traceabilty Direct analysis Application 1: ETV-ICP-MS of solid materias (polymers, powders, ceramics) Lecture 5: Application 2: Speciation Hyphenation Speciation HPLC-ICP-MS (RP, SEC, HILIC) FFF & AF4-ICP-MS GC-ICP-MS Applications: Selected by choice Lecture 6: Isotope ratio measurements and application 3: isotope dilution Isotope Analysis and Isotope Dilution Analysis, Uncertainty Budget Isotopic fractionation in bio-systems Application 3: Isotope Analysis & Provenance testing Lecture 7: Application 4: Life science applications (DNA, proteins and antibodies) LA-ICP-MS of Gels and Western Blots Metalloproteins Metal-Protein-Adducts Bioconjugation of proteins and antibodies Application 4: Proteins and Antibodies Lecture 8: Laser ablation and Application 5: Bioimaging Lecturer: Norbert Jakubowski Fundamentals of laser ablation Imaging (LA-ICP-MS) Internal standard Quantification approaches: matrix matching and aerosol mixing Bioimaging Biomarker Immunoassays Application 5: Bio-Imaging of cells and tissue Lecture 9: Laser ablation and Application 6: Polymers, ceramics and geological samples Line scanning versus hole drilling Fractionation Standard reference materials Calibration by matrix matched standards Calibration by pressed powder pellets Calibration by dried residues: total consumption concept Lecture 10: Detection of transient signals; Application 6: Single nanoparticle and single cell detection by ICP-MS Microdroplet generator Nanoparticle Single particle ICP-MS Single cell ICP-MS Single cell LA-ICP-MS CyTOFMS Application 6: Nanoparticles and Cells Summary, final discussion
Literature
  • THOMAS, Robert J. Practical guide to ICP-MS : a tutorial for beginners. 3rd ed. Boca Raton: CRC Press, 2013, xxvii, 418. ISBN 9781466555433. info
  • THOMAS, Robert J. Practical guide to ICP-MS. New York: Marcel Dekker, 2004, xii, 324. ISBN 0824753194. info
Teaching methods
Lectures, demonstration and instruction in laboratory.
Assessment methods
Final discussion of teacher with students.
Language of instruction
English
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught only once.
Note related to how often the course is taught: 23. 4. - 17. 5. 2019.
Teacher's information
Dr. Norbert Jakubowski Federal Institute for Materials Research and Testing BAM Berlin Studies: 1974 – 1977: “University of Essen”: BSc 1977 – 1981: “University of Essen”: MSc 1987 –1991: Thesis at the “University of Hohenheim”: Ph.D. Professional experience: 1981 – 1982: Scientific consultant of the „Gesellschaft der Förderer des Deutschen Energiezentrums (Society of the Promotion of a German Energy Centre)“ 1982 until 2009: Senior scientist at the ISAS - Institute for Analytical Sciences, Dortmund, Germany 2009 until now: Head of division “Inorganic Trace Analysis” at the BAM - Federal Institute for Materials Research and Testing, Berlin, Germany Awards 2005: Zimmer International Scholar given by the University of Cincinnati, USA 2006: Waters Symposium Prize: Pioneers in ICP-MS, Pittcon 2006, Orlando, USA 2013: European Award for Plasma Spectrochemistry, Krakow, Poland Boards and Committees: “Advisory Board” Member of the following journals: The Analyst, Journal of Metallomics Research interest and expertise: Analytical Chemistry with special interest in development of instruments, methods and problem-orientated procedures based on elemental mass spectrometry of solid and soft materials. Key Words: Speciation of P, Pt, Sn, Gd, Cd; metalloproteins, antibodies, bioconjugation by use of metals; analytical characterization of nanoparticles and their interaction with single biological cells, bioimaging of tissues by use of LA-ICP-MS. Link: https://www.bam.de/Navigation/DE/Ueber-die-BAM/Organisation/Organigramm/Praesident/Abteilung-1/Fachbereich-11/fachbereich11.html
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