Bi6336c Biology of germ-cells-practical lesson

Faculty of Science
Spring 2022
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. MVDr. Martin Anger, CSc. (seminar tutor)
Mgr. Jitka Danadová (seminar tutor)
Mgr. Bc. Kristína Kovačovicová, Ph.D. (seminar tutor)
Mgr. et Mgr. Martin Šodek (seminar tutor)
Guaranteed by
doc. MVDr. Martin Anger, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. MVDr. Martin Anger, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites (in Czech)
Bi4020c Molecular biology - practice || Bi4035 Molecular biology - lab.pract.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 1/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
Course objectives
The goal of this course is to train students in methods used in human assisted reproduction and research focused on mammalian germ cells and embryos.
Learning outcomes
At the end of the course students should have practical knowledge of: - methods used in isolation of mammalian germ cells and embryos - techniques of in vitro manipulation of germ cells and embryos - methods used in evaluation of male germ cells - basic methods used for analysis of chromosomes in germ cells and embryos including chromosomal spreads, immunofluorescence, laser microdissection, in situ hybridization, comparative genomic hybridization, Q-PCR - methods of widefield and confocal microscopy used in gamete research - microscopy of fixed samples, live cell imaging, FRET, FRAP and basic method of image analysis
Syllabus
  • 1. Isolation of mammalian oocytes and embryos in vitro 2. Basics of microinjection and micromanipulation of oocytes 3. Methods of evaluation of male germ cells 4. Basics and advanced methods used for analysis of chromosomes of germ cells and embryos - chromosomal spreads, immunofluorescence, laser microdissection, in situ hybridization, comparative genomic hybridization, qPCR 5. Methods and techniques of widefield and confocal microscopy used in research focused on germ cells and embryos - microscopy of fixed specimen, live cell imaging microscopy, FRET, FRAP, image analysis
Literature
  • ALBERTS, Bruce, Alexander JOHNSON, Julian LEWIS, David Owen MORGAN, Martin C. RAFF, Keith ROBERTS and Peter WALTER. Molecular biology of the cell. Edited by John H. Wilson - Tim Hunt. Sixth edition. New York, NY: Garland Science, 2014, xxxiv, 134. ISBN 9780815344322. info
Teaching methods
Content of this course is based on practical laboratory training and on demonstration of methods by lecturers.
Assessment methods
The course is completed by credit based on protocols provided by student.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
General note: Určeno pro doktorské a magisterské studium biologie.
The course is also listed under the following terms Spring 2013, Spring 2014, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2022/Bi6336c