EMAR0933c Methods of Assisted Reproduction III-practice

Faculty of Medicine
autumn 2024
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. MVDr. Aleš Hampl, CSc. (seminar tutor)
PharmDr. Zuzana Holubcová, Ph.D. (seminar tutor)
MUDr. Eva Mecová (seminar tutor)
MUDr. Volodymyr Porokh (seminar tutor)
RNDr. Gabriela Tauwinklová (seminar tutor)
Drahomíra Kyjovská (assistant)
Guaranteed by
doc. MVDr. Aleš Hampl, CSc.
Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Gabriela Tauwinklová
Supplier department: Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
EMAR0933c/01: Wed 14:00–17:20 F01B1/630
EMAR0933c/02: Wed 10:30–13:50 F01B1/630
Prerequisites
EMAR0832c
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main aim of the course is to introduce students to the practical aspects of basic and special methods of assisted reproduction. In the course of the exercises, students will learn micromanipulation of gametes and embryos, laboratory methods performed in the course of obtaining gametes, their evaluation and preparation for fertilization. Much attention will be paid to practical training in fertilization methods, embryo quality assessment, cryopreservation of gametes and embryos, and sample collection for genetic testing. Practical skills training will be a key element of the course.
Learning outcomes
Upon completion of the course, the student will:
- master the procedures of obtaining gametes and their preparation for fertilization.
- In addition, the student will gain experience in performing micromanipulation techniques including methods of fertilizing oocytes using intracytoplasmic sperm injection (ICSI) and collecting a sample for genetic testing.
- Able to independently assess gamete quality and gain an understanding of how to evaluate embryo development.
- Know how to prepare an embryo for transfer and know the basic procedures for cryopreservation of gametes and embryos.
- be able to adjust a microscope and micromanipulator.
Syllabus
  • - Basic and special AR methods
  • - Processing (ejaculate/testicular biopsy), preparation of sperm for in vitro fertilization, determination and evaluation of spermiogram
  • - Search and evaluation of oocyte-cumulus complex, oocyte denudation, evaluation of oocyte maturity and quality, in vitro follicle maturation, polarizing microscopy
  • - Conventional in vitro fertilization
  • - Fertilization by intracytoplasmic sperm injection ICSI, special methods of sperm injection (PICSI, IMSI)
  • - Classical and extended embryo culture, individual and group culture, assisted hatching
  • - Evaluation of embryo development - basic morphological criteria, evaluation of morphokinetic parameters (time lapse systems)
  • - Practical training on biopsy of polar body, blastomeres and trophoectoderm for genetic examination
  • - Embryo transfer - practice with the transfer catheter
  • - Methods of cryopreservation (slow freezing, vitrification; open and closed freezing systems), practical training in freezing and thawing, sperm, embryos, oocytes and reproductive tissues; safety of working with liquid nitrogen
  • - Documentation in the laboratory
Literature
    recommended literature
  • The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. In Hum Reprod. 2011 Jun;26(6):1270-83.
  • http://atlas.eshre.eu/
  • WHO laboratory manual for the examination and processing of human semen, 5th ed., 2010.
  • Elder K.: In Vitro Fertilization. 3rd ed. Cambridge 2011.
  • Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology:
  • Magli, C, Jones,G.M., Lundin,K., Van den Abbeel E. and The Special Interest Group on Embryology: Atlas 27, Suppl. 1.
  • Coward K., Wells D.: Textbook of Clinical Embryology, 1st ed., Cambridge 2013.
  • In vitro fertilization. Edited by Kay Elder - Brian Dale. 2nd ed. New York: Cambridge University Press, 2000, xii, 310 p. ISBN 0521778638. info
Teaching methods
100% participation in the exercises is required. Completion is by practical examination and credit.
Assessment methods
The main element of the course will be practical teaching in the teaching laboratory supplemented by an excursion to the clinical embryology laboratory, during the practical teaching mouse oocytes, embryos, and reproductive organs (or biological material from other species) will be used.
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on the extent and intensity of the course: 60.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/med/autumn2024/EMAR0933c