Course Information

aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
autumn 2023

Extent and Intensity

0/4/0. 7 credit(s). Type of Completion: z (credit).
Taught in person.

Teacher(s)

doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)

Guaranteed by

doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: MUDr. Michaela Králíková, Ph.D.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable of Seminar Groups

aVLBC0321s/30: Tue 14:00–15:40 A16/213, Fri 14:00–15:40 A16/215, J. Slanina
aVLBC0321s/31: Tue 14:00–15:40 A16/213, Fri 8:00–9:40 A16/213, H. Paulová, J. Slanina
aVLBC0321s/32: Mon 10:00–11:40 A16/213, Fri 10:00–11:40 A16/213, E. Táborská
aVLBC0321s/33: Mon 10:00–11:40 A16/213, Fri 10:00–11:40 A16/213, E. Táborská
aVLBC0321s/34: Tue 8:00–9:40 A16/215, Fri 14:00–15:40 A16/215, H. Paulová, J. Slanina
aVLBC0321s/35: Tue 8:00–9:40 A16/215, Fri 8:00–9:40 A16/213, H. Paulová
aVLBC0321s/36: Tue 16:00–17:40 A16/213, Fri 10:00–11:40 A16/215, J. Dostál, J. Tomandl
aVLBC0321s/37: Tue 16:00–17:40 A16/213, Fri 10:00–11:40 A16/215, J. Dostál, J. Tomandl
aVLBC0321s/38: Tue 15:00–16:40 A16/215, Thu 15:00–16:40 A16/213, J. Dostál, J. Tomandl
aVLBC0321s/39: Tue 15:00–16:40 A16/215, Thu 15:00–16:40 A16/213, J. Dostál, J. Tomandl

Prerequisites (in Czech)

aVLBI0222p Medical Biology II - lect. && aVLBF011p Biophysics -lect.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (programme LF, M-GM)
General Medicine (eng.) (programme LF, M-VL)

Course objectives

The aim of the course is to practice basic metabolic processes and pathways occurring at the cellular level.
Understanding these processes is the basis for understanding metabolism at the tissue and organ level, which is the focus of the follow-up course Biochemistry II.

Learning outcomes

At the end of the course, students will:
- understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
- discuss the properties and function of enzymes
- describe basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level, and the transport processes on the membrane.
- describe protein synthesis, starting with replication, transcription, translation, and post-translational modifications. Understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discuss the principles of some diseases at the molecular level.

Syllabus

Introduction to biochemistry. Electrolytes, ion activity, osmolarity, osmolality, tonicity, oncotic pressure.
Acid-base reactions, weak acids/bases. Buffers, Henderson-Hasselbalch equation. Properties of organic compounds (alcohols, aldehydes, ketones, carboxylic acids and their derivatives).
Introduction to bioenergetics, Gibbs energy. High-energy compounds. Redox reaction.
Enzymes – activity, enzyme kinetics, enzyme kinetic parameters, enzyme inhibition.
Enzyme cofactors – main features of structure, functions, and relationship to vitamins.
Citrate cycle, respiratory chain, oxidative phosphorylation.
Structure and properties of carbohydrates. Carbohydrates in nutrition. Carbohydrate digestion. Glucose transport into cells.
Glycolysis. Gluconeogenesis.
Glycogen metabolism. Pentose phosphate pathway. Metabolism of fructose and galactose.
Structure and properties of lipids. Surfactants. Cell membranes, membrane transport.
Lipids in nutrition. Digestion of lipids. Lipids in nutrition. Breakdown of fatty acids. Metabolism of ketone bodies.
Synthesis, elongation and desaturation of fatty acids.
Cholesterol metabolism. Bile acid metabolism.
Metabolism of triacylglycerols, phospholipids.
Lipid peroxidation. Eicosanoids. Lipophilic vitamins. Reactive oxygen species.
Structure and function of hemoglobin
Structure and properties of amino acids, peptides, proteins. Protein digestion. Overview of protein metabolism.
General features of conversion amino acids. Urea synthesis.
Catabolism of amino acids. Synthesis of non-essential amino acids, reversible transformations.
Conversions of amino acids into specialized products. Heme synthesis.
Structure and significance of bases/nucleotides. Uric acid. Common features of nucleotide biosynthesis.
Metabolism of purine/pyrimidine nucleotides. The importance of tetrahydrofolate and vitamin B12.
Structure of DNA, RNA, replication, transcription, protein synthesis, post-translational modification of proteins.

Literature

required literature

Interactive Syllabus of the course in IS MUNI.
RODWELL, Victor W., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY and P. Anthony WEIL. Harper's illustrated biochemistry. Thirty-first edition. New York: McGraw-Hill, 2018, x, 789. ISBN 9781260288421. info
Essentials of Medical Chemistry and Biochemistry (Ed. Dostál J.). Download: https://portal.med.muni.cz/article-623-essentials-of-medical-chemistry-and-biochemistry.html

recommended literature

LIEBERMAN, Michael and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 5th edition. Philadelphia: Wolters Kluwer, 2018, xii, 1051. ISBN 9781496387721. info
KOOLMAN, Jan and Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info
VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Textbook of biochemistry for medical students. Eighth edition. New Delhi: Jaypee The Health Sciences Publisher, 2016, xvi, 718. ISBN 9789385999741. info

Teaching methods

Active work in seminars, problem solving, discussions.

Assessment methods

Credit. Conditions for giving the course-unit credit:
1) completion of all seminars and 2) completion of in-term tests.
Failure to meet the requirements of in-term tests requires a resit test, which may be repeated only once. All absences must be made up before a resit test.
Detailed conditions are published in Study materials posted under the course/Interactive Syllabus.

Language of instruction

English

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 60.

Listed among pre-requisites of other courses

The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022.

aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
autumn 2022

Extent and Intensity

0/4/0. 7 credit(s). Type of Completion: z (credit).
Taught in person.

Teacher(s)

Guaranteed by

doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable of Seminar Groups

aVLBC0321s/30: Mon 16:00–17:40 A16/213, Thu 17:00–18:40 A16/215, J. Dostál, J. Slanina
aVLBC0321s/31: Tue 10:00–11:40 A16/215, Thu 17:00–18:40 A16/215, J. Slanina
aVLBC0321s/32: Tue 14:00–15:40 A16/215, Fri 14:00–15:40 A16/213, E. Táborská
aVLBC0321s/33: Tue 14:00–15:40 A16/215, Fri 14:00–15:40 A16/213, E. Táborská
aVLBC0321s/34: Tue 8:00–9:40 A16/215, Thu 8:00–9:40 A16/215, H. Paulová
aVLBC0321s/35: Tue 8:00–9:40 A16/215, Thu 8:00–9:40 A16/215, H. Paulová
aVLBC0321s/36: Tue 10:00–11:40 A16/215, Thu 13:00–14:40 A16/215, J. Dostál, J. Slanina
aVLBC0321s/37: Mon 16:00–17:40 A16/213, Thu 13:00–14:40 A16/215, J. Dostál
aVLBC0321s/38: Mon 10:00–11:40 A16/215, Fri 12:00–13:40 A16/215, J. Tomandl
aVLBC0321s/39: Mon 10:00–11:40 A16/215, Fri 12:00–13:40 A16/215, J. Tomandl

Prerequisites (in Czech)

aVLBI0222p Medical Biology II - lect. && aVLBF011p Biophysics -lect.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (programme LF, M-GM)
General Medicine (eng.) (programme LF, M-VL)

Course objectives

The aim of the course is to obtain knowledge on essential metabolic processes on the cellular level. Understanding these processes is a base for comprehension of metabolism on the tissue and organ level. Introductory lessons summarize the basic terms of chemistry needed for an understanding of body structure a physico-chemical processes occurring in it (chemical composition of the body, biologically important elements, water, electrolytes, non-electrolytes, osmotic pressure, acid-base, redox and precipitation reactions), the following lectures are focused on biochemical pathways in cells.

Learning outcomes

At the end of the course, students will be competent to:
- understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
- describe the role of macro/microbiogenic elements in the body.
- discuss the properties and function of enzymes
- describe basic catabolic and anabolic pathways of carbohydrates, lipids, and protein metabolism, and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications.
- understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discuss the principles of some diseases at the molecular level.

Syllabus

Electrolytes, osmolality, tonicity, oncotic pressure, osmolal gap.
Acid-base reactions, pH of body fluids.
Buffers, Henderson-Hasselbalch equation.
Biochemically important organic compounds I (alcohols, aldehydes).
Biochemically important organic compounds II (carboxylic acids and derivatives).
Bioenergetics, Gibbs energy, ATP, redox reactions.
Enzymes, kinetics, saturation curve, inhibition.
Cofactors of enzymes, relation to vitamins.
Citrate cycle, respiratory chain.
Saccharides, structures, nutrition, digestion. Transport of glucose into cells. Glycolysis.
Metabolism of glycogen. Metabolism of fructose and galactose.
Surfactants (types, physiological roles). Lipids (structures).
Biological membranes. Membrane transport.
Lipids in nutrition, digestion. Catabolism of fatty acids, ketone bodies.
Desaturation of fatty acids. Cholesterol.
Metabolism of bile acids, eicosanoids, phospholipids.
Lipophilic vitamins, ROS. Lipid peroxidation.
Amino acids, proteins, structure, properties.
Hemoglobin, types, function, abnormal types.
Digestion of proteins, general features of amino acid metabolism, synthesis of urea.
Metabolism of amino acids I (catabolism, synthesis of non-essential AA, congenital disorders).
Metabolism of amino acids II (conversions to special products, heme).
Structure of bases, nucleosides, nucleotides, roles of nucleotides. Metabolism of purine and pyrimidine bases I.
Metabolism of purine and pyrimidine bases II.
DNA, RNA structure, replication, transcription.
Protein synthesis. Post-translation modification, collagen synthesis.

Literature

required literature

Seminar texts available in Information system
RODWELL, Victor W., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY and P. Anthony WEIL. Harper's illustrated biochemistry. Thirty-first edition. New York: McGraw-Hill, 2018, x, 789. ISBN 9781260288421. info

recommended literature

LIEBERMAN, Michael and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 5th edition. Philadelphia: Wolters Kluwer, 2018, xii, 1051. ISBN 9781496387721. info
KOOLMAN, Jan and Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info
VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Textbook of biochemistry for medical students. Eighth edition. New Delhi: Jaypee The Health Sciences Publisher, 2016, xvi, 718. ISBN 9789385999741. info

Teaching methods

Seminar. The course is based on group discussion on the given topics. The outlines of discussion are in the recommended textbook. Complementary materials are available in section Study materials in IS MUNI.

Assessment methods

Course-unit credit. Conditions for giving the course-unit credit (both conditions should be met):

Full attendance in lessons. All absences should be justified and then made up.

At least 70% success rate from partial revision tests or 50% success rate in credit test, the resit credit test can be taken only once.
The detailed conditions are posted in Information System (Study materials – Course-related instructions).

Language of instruction

English

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 60.

Listed among pre-requisites of other courses

The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2023.

aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
autumn 2021

Extent and Intensity

0/4/0. 7 credit(s). Type of Completion: z (credit).
Taught in person.

Teacher(s)

Guaranteed by

doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: prof. RNDr. Eva Táborská, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable of Seminar Groups

aVLBC0321s/28: Tue 10:00–11:40 A16/213, Fri 8:00–9:40 A16/213, J. Dostál
aVLBC0321s/29: Tue 10:00–11:40 A16/213, Fri 8:00–9:40 A16/213, J. Dostál
aVLBC0321s/30: Tue 8:00–9:40 A16/215, Fri 8:00–9:40 A16/215, H. Paulová, J. Tomandl
aVLBC0321s/31: Tue 8:00–9:40 A16/215, Fri 8:00–9:40 A16/215, H. Paulová, J. Tomandl
aVLBC0321s/32: Tue 14:00–15:40 A16/213, Fri 14:00–15:40 A16/215, H. Paulová, E. Táborská
aVLBC0321s/33: Tue 14:00–15:40 A16/213, Fri 14:00–15:40 A16/215, H. Paulová, E. Táborská
aVLBC0321s/34: Tue 10:00–11:40 A16/215, Thu 10:00–11:40 A16/213, E. Táborská, J. Tomandl
aVLBC0321s/35: Tue 10:00–11:40 A16/215, Thu 10:00–11:40 A16/213, E. Táborská, J. Tomandl
aVLBC0321s/36: Mon 14:00–15:40 A16/215, Wed 8:00–9:40 A16/215, H. Paulová, J. Slanina
aVLBC0321s/37: Mon 14:00–15:40 A16/215, Wed 8:00–9:40 A16/215, H. Paulová, J. Slanina
aVLBC0321s/38: Tue 16:00–17:40 A16/213, Thu 16:00–17:40 A16/213, J. Slanina, J. Tomandl
aVLBC0321s/39: Tue 16:00–17:40 A16/213, Thu 16:00–17:40 A16/213, J. Slanina, J. Tomandl

Prerequisites (in Czech)

aVLBI0222p Medical Biology II - lect. && aVLBF011p Biophysics -lect.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (programme LF, M-GM)
General Medicine (eng.) (programme LF, M-VL)

Course objectives

Learning outcomes

Syllabus

Electrolytes, osmolality, tonicity, oncotic pressure, osmolal gap.
Acid-base reactions, pH of body fluids.
Buffers, Henderson-Hasselbalch equation.
Biochemically important organic compounds I (alcohols, aldehydes).
Biochemically important organic compounds II (carboxylic acids and derivatives).
Bioenergetics, Gibbs energy, ATP, redox reactions.
Enzymes, kinetics, saturation curve, inhibition.
Cofactors of enzymes, relation to vitamins.
Citrate cycle, respiratory chain.
Saccharides, structures, nutrition, digestion. Transport of glucose into cells. Glycolysis.
Metabolism of glycogen. Metabolism of fructose and galactose.
Surfactants (types, physiological roles). Lipids (structures).
Biological membranes. Membrane transport.
Lipids in nutrition, digestion. Catabolism of fatty acids, ketone bodies.
Desaturation of fatty acids. Cholesterol.
Metabolism of bile acids, eicosanoids, phospholipids.
Lipophilic vitamins, ROS. Lipid peroxidation.
Amino acids, proteins, structure, properties.
Hemoglobin, types, function, abnormal types.
Digestion of proteins, general features of amino acid metabolism, synthesis of urea.
Metabolism of amino acids I (catabolism, synthesis of non-essential AA, congenital disorders).
Metabolism of amino acids II (conversions to special products, heme).
Structure of bases, nucleosides, nucleotides, roles of nucleotides. Metabolism of purine and pyrimidine bases I.
Metabolism of purine and pyrimidine bases II.
DNA, RNA structure, replication, transcription.
Protein synthesis. Post-translation modification, collagen synthesis.

Literature

required literature

Seminar texts available in Information system
RODWELL, Victor W., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY and P. Anthony WEIL. Harper's illustrated biochemistry. Thirty-first edition. New York: McGraw-Hill, 2018, x, 789. ISBN 9781260288421. info

recommended literature

LIEBERMAN, Michael, Allan D. MARKS and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 4th ed. Baltimore: Lippincott, Williams & Wilkins, 2013, ix, 1014. ISBN 9781451100037. info
KOOLMAN, Jan and Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info
VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Textbook of biochemistry for medical students. Eighth edition. New Delhi: Jaypee The Health Sciences Publisher, 2016, xvi, 718. ISBN 9789385999741. info

Teaching methods

Assessment methods

Course-unit credit. Conditions for giving the course-unit credit (both conditions should be met):

Full attendance in lessons. All absences should be justified and then made up.

Language of instruction

English

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 60.

Listed among pre-requisites of other courses

The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2022, autumn 2023.

aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
autumn 2020

Extent and Intensity

0/2/0. 4 credit(s). Type of Completion: z (credit).
Taught partially online.

Teacher(s)

doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: Monika Šudáková
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable of Seminar Groups

aVLBC0321s/30: Tue 16:00–17:40 A16/215, J. Tomandl
aVLBC0321s/31: Tue 16:00–17:40 A16/215, J. Tomandl
aVLBC0321s/32: Tue 12:00–13:40 A16/213, E. Táborská
aVLBC0321s/33: Tue 12:00–13:40 A16/213, E. Táborská
aVLBC0321s/34: Tue 8:00–9:40 A16/213, H. Paulová
aVLBC0321s/35: Tue 8:00–9:40 A16/213, H. Paulová
aVLBC0321s/36: Tue 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/37: Tue 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/38: Mon 14:00–15:40 A16/215, J. Slanina
aVLBC0321s/39: Mon 14:00–15:40 A16/215, J. Slanina

Prerequisites (in Czech)

aVLBI0222c Medical Biology II - pract. && aVLBF011c Biophysics - pract.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (programme LF, M-GM)
General Medicine (eng.) (programme LF, M-VL)
General Medicine (programme LF, M-VL) (2)

Course objectives

Seminars (A) and practicals (B) have a common content. The aim of the course is to obtain knowledge on essential metabolic processes on the cellular level. Understanding of these proceses is a base for comprihension of metabolism on the tissue and organ level. Introductory lessons summarize the basic terms of chemistry needed for understanding of body structure a physico-chemical processes occuring in it (chemical composition of the body, biologically important elements, water, electrolytes, non-electrolytes, osmotic pressure, acid-base, redox and precipitation reactions), the following lectures are focused on biochemichal pathways in cells.

Learning outcomes

At the end of the course students will be competent to:
- understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
- describe the role of macro/microbiogenic elements in the body.
- discusse the properties and function of enzymes
- describe basic catabolic and anabolic pathways of carbohydrates, lipids, and protein metabolism, and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications. Understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discusse the principles of some diseases at the molecular level.

Syllabus

1A: Introduction to biochemistry.
1B: Electrolytes, osmolality, tonicity, oncotic pressure, osmolal gap.
2A: Acid-base reactions, pH of body fluids.
2B: Buffers, Henderson-Hasselbalch equation.
3A: TEST 1 (sem 1A-2B). Biochemically important organic compounds I (alcohols, aldehydes).
3B: Biochemically important organic compounds II (carboxylic acids and derivatives).
4A: Bioenergetics, Gibbs energy, ATP, redox reactions.
4B: Enzymes, kinetics, saturation curve, inhibition.
5A: Cofactors of enzymes, relation to vitamins.
5B: Citrate cycle, respiratory chain.
6A: Saccharides, structures, nutrition, digestion. Transport of glucose into cells. Glycolysis.
6B: Metabolism of glycogen. Metabolism of fructose and galactose.
7A: TEST 2 (sem 3A-6B). Surfactants (types, physiological roles). Lipids (structures).
7B: Cell membranes, transport.
8A: Lipids in nutrition, digestion. Catabolism of fatty acids, ketone bodies.
8B: Desaturation of fatty acids. Cholesterol.
9A: Metabolism of bile acids, eicosanoids, phospholipids.
9B: Lipophilic vitamins, ROS. Lipoperoxidation.
10A: TEST 3 (sem 7A-9B). Amino acids, proteins, structure, properties.
10B: Hemoglobin, types, function, abnormal types.
11A: Digestion of proteins, general features of amino acid metabolism, synthesis of urea.
11B: Metabolism of amino acids I (catabolism, synthesis of non-essential AA, congenital disorders).
12A: Metabolism of amino acids II (conversions to special products, heme).
12B: TEST 4 (sem 10A-12A). Structure of bases, nucleosides, nucleotides, roles of nucleotides. Metabolism of purine and pyrimidine bases I.
13A: Metabolism of purine and pyrimidine bases II.
13B: DNA, RNA structure, replication, transcription.
14A: Protein synthesis. Post-translation modification, collagen synthesis. Credit test.
14B: Consultations, compensatory lessons.

Literature

required literature

Seminar texts available in Information system
Lecture files including lecture notes available in IS
RODWELL, Victor W., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY and P. Anthony WEIL. Harper's illustrated biochemistry. Thirty-first edition. New York: McGraw-Hill, 2018, x, 789. ISBN 9781260288421. info
KOOLMAN, Jan and Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info

Teaching methods

Course is based on group discusion to the given topics. The outlines of discusion are in the recommended textbook. Complementary materials are available in section Study materials.

Assessment methods

Full attendance in seminars is the principal condition. If any absence, it must be apologized through Department of Study Affairs up to five days. If apology is recorded in Information System, then student is allowed to make up the absence according to teacher's instructions. Four revision tests are written in seminars, semestral limit for credit is 42. If the semestral limit is not fulfilled, student must write the Credit test (limit 14/30). All absences must be made up before writing the credit test. One repetition of the Credit test is approved. Depending on epidemiological situation, assessment method may be altered. The current conditions will be posted in IS (Study materials – Course-related instructions).

Language of instruction

English

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 30.

Listed among pre-requisites of other courses

The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2021, autumn 2022, autumn 2023.

aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
autumn 2019

Extent and Intensity

0/2/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (assistant)
PharmDr. Katarína Kostolanská (assistant)
Lenka Nerudová (assistant)
Mgr. Roman Sándor, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable of Seminar Groups

aVLBC0321s/30: Tue 16:00–17:40 A16/213, J. Tomandl
aVLBC0321s/31: Tue 16:00–17:40 A16/213, J. Tomandl
aVLBC0321s/32: Tue 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/33: Tue 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/34: Mon 16:00–17:40 A16/215, H. Paulová
aVLBC0321s/35: Mon 16:00–17:40 A16/215, H. Paulová
aVLBC0321s/36: Tue 12:00–13:40 A16/215
aVLBC0321s/37: Tue 12:00–13:40 A16/215
aVLBC0321s/38: Mon 13:00–14:40 A16/215, J. Slanina
aVLBC0321s/39: Mon 13:00–14:40 A16/215, J. Slanina

Prerequisites (in Czech)

aVLBI0222c Biology II-pract. && aVLBF011c Biophysics - pract.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (eng.) (programme LF, M-VL)
General Medicine (programme LF, M-VL)

Course objectives

Learning outcomes

Syllabus

1A: Introduction to biochemistry.
1B: Electrolytes, osmolality, tonicity, oncotic pressure, osmolal gap.
2A: Acid-base reactions, pH of body fluids.
2B: Buffers, Henderson-Hasselbalch equation.
3A: TEST 1 (sem 1A-2B). Biochemically important organic compounds I (alcohols, aldehydes).
3B: Biochemically important organic compounds II (carboxylic acids and derivatives).
4A: Bioenergetics, Gibbs energy, ATP, redox reactions.
4B: Enzymes, kinetics, saturation curve, inhibition.
5A: Cofactors of enzymes, relation to vitamins.
5B: Citrate cycle, respiratory chain.
6A: Saccharides, structures, nutrition, digestion. Transport of glucose into cells. Glycolysis.
6B: Metabolism of glycogen. Metabolism of fructose and galactose.
7A: TEST 2 (sem 3A-6B). Surfactants (types, physiological roles). Lipids (structures).
7B: Cell membranes, transport.
8A: Lipids in nutrition, digestion. Catabolism of fatty acids, ketone bodies.
8B: Desaturation of fatty acids. Cholesterol.
9A: Metabolism of bile acids, eicosanoids, phospholipids.
9B: Lipophilic vitamins, ROS. Lipoperoxidation.
10A: TEST 3 (sem 7A-9B). Amino acids, proteins, structure, properties.
10B: Hemoglobin, types, function, abnormal types.
11A: Digestion of proteins, general features of amino acid metabolism, synthesis of urea.
11B: Metabolism of amino acids I (catabolism, synthesis of non-essential AA, congenital disorders).
12A: Metabolism of amino acids II (conversions to special products, heme).
12B: TEST 4 (sem 10A-12A). Structure of bases, nucleosides, nucleotides, roles of nucleotides. Metabolism of purine and pyrimidine bases I.
13A: Metabolism of purine and pyrimidine bases II.
13B: DNA, RNA structure, replication, transcription.
14A: Protein synthesis. Post-translation modification, collagen synthesis. Credit test.
14B: Consultations, compensatory lessons.

Literature

required literature

Lecture files including lecture notes available in IS
Seminar texts available in Information system
RODWELL, Victor W., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY and P. Anthony WEIL. Harper's illustrated biochemistry. Thirty-first edition. New York: McGraw-Hill, 2018, x, 789. ISBN 9781260288421. info
KOOLMAN, Jan and Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info

Teaching methods

Course is based on group discusion to the given topics. The outlines of discusion are in the recommended textbook. Complementary materials are available in section Study materials.

Assessment methods

Language of instruction

English

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 30.

Listed among pre-requisites of other courses

The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
autumn 2018

Extent and Intensity

0/2/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

Ing. Martina Čarnecká, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (assistant)
PharmDr. Katarína Kostolanská (assistant)
Lenka Nerudová (assistant)
Mgr. Lucie Novotná (assistant)
Mgr. Anna Pleskačová, Ph.D. (assistant)
Mgr. Roman Sándor, Ph.D. (assistant)

Guaranteed by

Timetable of Seminar Groups

aVLBC0321s/30: Tue 11:00–12:40 A16/215
aVLBC0321s/31: Mon 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/32: Mon 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/33: Mon 16:00–17:40 A16/213, E. Táborská
aVLBC0321s/34: Tue 11:00–12:40 A16/213
aVLBC0321s/35: Tue 16:00–17:40 A16/213, J. Tomandl
aVLBC0321s/36: Tue 16:00–17:40 A16/213, J. Tomandl
aVLBC0321s/37: Mon 16:00–17:40 A16/213, E. Táborská
aVLBC0321s/38: Tue 11:00–12:40 A16/215

Prerequisites (in Czech)

aVLBI0222c Biology II - pract. && aVLBF011c Biophysics - pract.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (eng.) (programme LF, M-VL)
General Medicine (programme LF, M-VL)

Course objectives

Seminars and exercises have a common content and serve for active practice of the subject matter in Biochemistry I. The aim of the course is to obtain knowledge on essential metabolic processes on the cellular level. Understanding of these proceses is a base for comprihension of metabolism on the tissue and organ level. In the introductory lessons are summarized basic terms from chemistry needed for understanding of body structure a physico-chemical processes occuring in it ((chemical composition of the body, survay of biologically important elements,water, elektrolytes, non-elektrolytes, osmotic pressure, acid-base, redox and precipitation reactions), the following lectures are focused on biochemichal pathways in cells.

Learning outcomes

In the end of the course will students understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
Describes the role of macro- and microbiogenic elements in the organism
Discusses the properties and function of enzymes
Describes basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism, and their relationships.
Understands the principles of energy production, utilization and deposition at the cellular level.
Explains the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
Describes protein synthesis, starting with the replication and transcription, translation and post-translational modifications. Understands the relationship between protein structure and function.
Explains the function of hemoglobin in oxygen transport and maintaining acid-base balance.
Discusses the principles of some diseases at the molecular level.

Syllabus

Chemical composition of human body.
Water, electrolytes, osmotic pressure, osmolality. pH, buffers. Structure of haemoglobin and its relationship to the function. Hb types in the blood of healthy subjects, HbCO and MetHb, abnormal Hb types. Enzymes - reaction rate, progress curve, the Michaelis plot and Km, enzyme inhibition. Coenzymes, their relationship to vitamins. Membrane structure and assembly. Transport across membranes. Metabolism of glucose: Glycolysis under anaerobic and aerobic conditions and the oxidation of pyruvate. Gluconeogenesis. Glycogenesis and glycogenolysis. Metabolism of proteins. Common features of amino acid conversion. The synthesis of urea. Nitrogen balance. Important reactions in amino acid catabolism. Biosynthesis and desaturation of fatty acids. The sources of essential fatty acids. Metabolism of triacylglycerols. Metabolism of phospholipids. Biosynthesis of eicosanoids. Peroxidation of lipids. The citric acid cycle. The respiratory chain and oxidative phosphorylation. Replication, transcription, proteosynthesis.

Literature

required literature

Seminar texts available in Information system

Teaching methods

Course is based on group discusion to the given topics. The outlines of discusion are in the recommended textbook. Complementary materials are available in section Study materials.

Assessment methods

Language of instruction

English

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 30.

Listed among pre-requisites of other courses

The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
Autumn 2017

Extent and Intensity

0/2/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

Guaranteed by

Timetable of Seminar Groups

aVLBC0321s/30: Tue 10:30–12:10 A16/215, H. Paulová
aVLBC0321s/31: Mon 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/32: Mon 14:00–15:40 A16/215, J. Dostál
aVLBC0321s/33: Mon 16:00–17:40 A16/215, E. Táborská
aVLBC0321s/34: Tue 10:30–12:10 A16/215, H. Paulová
aVLBC0321s/35: Tue 16:40–18:20 A16/215, J. Tomandl
aVLBC0321s/36: Tue 16:40–18:20 A16/215, J. Tomandl
aVLBC0321s/37: Mon 16:00–17:40 A16/215, E. Táborská
aVLBC0321s/38: Tue 10:30–12:10 A16/215, J. Slanina

Prerequisites (in Czech)

aVLBI0222c Biology II - pract. && aVLBF011c Biophysics - pract.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (eng.) (programme LF, M-VL)
General Medicine (programme LF, M-VL)

Course objectives

Learning outcomes

Syllabus

Chemical composition of human body.
Water, electrolytes, osmotic pressure, osmolality. pH, buffers. Structure of haemoglobin and its relationship to the function. Hb types in the blood of healthy subjects, HbCO and MetHb, abnormal Hb types. Enzymes - reaction rate, progress curve, the Michaelis plot and Km, enzyme inhibition. Coenzymes, their relationship to vitamins. Membrane structure and assembly. Transport across membranes. Metabolism of glucose: Glycolysis under anaerobic and aerobic conditions and the oxidation of pyruvate. Gluconeogenesis. Glycogenesis and glycogenolysis. Metabolism of proteins. Common features of amino acid conversion. The synthesis of urea. Nitrogen balance. Important reactions in amino acid catabolism. Biosynthesis and desaturation of fatty acids. The sources of essential fatty acids. Metabolism of triacylglycerols. Metabolism of phospholipids. Biosynthesis of eicosanoids. Peroxidation of lipids. The citric acid cycle. The respiratory chain and oxidative phosphorylation. Replication, transcription, proteosynthesis.

Literature

required literature

Seminar texts available in Information system

Teaching methods

Course is based on group discusion to the given topics. The outlines of discusion are in the recommended textbook. Complementary materials are available in section Study materials.

Assessment methods

Language of instruction

English

Further Comments

The course is taught annually.

Listed among pre-requisites of other courses

The course is also listed under the following terms Autumn 2016, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
Autumn 2016

Extent and Intensity

0/2/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

Guaranteed by

Timetable of Seminar Groups

Prerequisites (in Czech)

aVLBI0222c Biology II - pract. && aVLBF011c Biophysics - pract.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (eng.) (programme LF, M-VL)
General Medicine (programme LF, M-VL)

Course objectives

The aim of the course is to obtain knowledge on essential metabolic processes on the cell level. Understanding of these proceses is a base for comprihension of metabolism on the tissue and organ level. In the introductory lessons are summarized basic terms from chemistry needed for understanding of body structure a physico-chemical processes occuring in it ((chemical composition of the body, survay of biologically important elements,water, elektrolytes, non-elektrolytes, osmotic pressure, acid-base, redox and precipitation reactions), the following lectures are focused on biochemichal pathways in cells. The graduate is acquainted with principles of acid-base, precipitation and redox reactions, role of macro- and micro elements in the body, properties and function of enzymes, is oriented in catabolic and anabolic pathways of metabolism of saccharides, lipids and proteins and their consequences.Understands the principles of cell bioenegetics.Become familiar with the funcion of cell membranes, principle of cell compartmentation and membrane transport.Has knowledge on proteosynthesis including processes replication,transcription, translation and posttranslantion modifications.Understands the relation between the structure and function of proteins. Is acquinted with function of hemoglobine in oxygen transport.Molecular bases of some diseases are introduced. The course provides the essential knowledge for future understanding of metabolism on organe and inter-organe level and its disturbances.

Syllabus

Chemical composition of human body.
Water, electrolytes, osmotic pressure, osmolality. pH, buffers. Structure of haemoglobin and its relationship to the function. Hb types in the blood of healthy subjects, HbCO and MetHb, abnormal Hb types. Enzymes - reaction rate, progress curve, the Michaelis plot and Km, enzyme inhibition. Coenzymes, their relationship to vitamins. Membrane structure and assembly. Transport across membranes. Metabolism of glucose: Glycolysis under anaerobic and aerobic conditions and the oxidation of pyruvate. Gluconeogenesis. Glycogenesis and glycogenolysis. Metabolism of proteins. Common features of amino acid conversion. The synthesis of urea. Nitrogen balance. Important reactions in amino acid catabolism. Biosynthesis and desaturation of fatty acids. The sources of essential fatty acids. Metabolism of triacylglycerols. Metabolism of phospholipids. Biosynthesis of eicosanoids. Peroxidation of lipids. The citric acid cycle. The respiratory chain and oxidative phosphorylation. Replication, transcription, proteosynthesis.

Teaching methods

Course is based on group discusion to the given topics. The outlines of discusion are in the recommended textbook. Complementary materials are available in section Study materials.

Assessment methods (in Czech)

Full (100%) attendance in seminars is the principal condition. If any absence, it must be apologized through Department of Study Affairs up to five days. If apology is recorded in Information System, then student is allowed to make up the absence according to teacher's instructions. Four revision tests are written in seminars, semestral limit for credit is 42. If the semestral limit is not fulfilled, student must write the Credit test (limit 14/30). All absences must be made up before writing the credit test. One repetition of the Credit test is approved.

Language of instruction

English

Further Comments

The course is taught annually.

Listed among pre-requisites of other courses

The course is also listed under the following terms Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

Enrolment Statistics (recent)

Course Information

aVLBC0321s Biochemistry I - seminar

aVLBC0321s Biochemistry I - seminar

aVLBC0321s Biochemistry I - seminar

aVLBC0321s Biochemistry I - seminar

aVLBC0321s Biochemistry I - seminar

aVLBC0321s Biochemistry I - seminar

aVLBC0321s Biochemistry I - seminar

aVLBC0321s Biochemistry I - seminar

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