aVLBC0321s Biochemistry I - seminar

Faculty of Medicine
autumn 2022
Extent and Intensity
0/4/0. 7 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Mgr. Ondřej Peš, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Contact Person: prof. RNDr. Eva Táborská, CSc.
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Prerequisites (in Czech)
aVLBI0222p Medical Biology II - lect. && aVLBF011p Biophysics -lect.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to obtain knowledge on essential metabolic processes on the cellular level. Understanding these processes is a base for comprehension of metabolism on the tissue and organ level. Introductory lessons summarize the basic terms of chemistry needed for an understanding of body structure a physico-chemical processes occurring in it (chemical composition of the body, biologically important elements, water, electrolytes, non-electrolytes, osmotic pressure, acid-base, redox and precipitation reactions), the following lectures are focused on biochemical pathways in cells.
Learning outcomes
At the end of the course, students will be competent to:
- understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
- describe the role of macro/microbiogenic elements in the body.
- discuss the properties and function of enzymes
- describe basic catabolic and anabolic pathways of carbohydrates, lipids, and protein metabolism, and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications.
- understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discuss the principles of some diseases at the molecular level.
Syllabus
  • Electrolytes, osmolality, tonicity, oncotic pressure, osmolal gap.
  • Acid-base reactions, pH of body fluids.
  • Buffers, Henderson-Hasselbalch equation.
  • Biochemically important organic compounds I (alcohols, aldehydes).
  • Biochemically important organic compounds II (carboxylic acids and derivatives).
  • Bioenergetics, Gibbs energy, ATP, redox reactions.
  • Enzymes, kinetics, saturation curve, inhibition.
  • Cofactors of enzymes, relation to vitamins.
  • Citrate cycle, respiratory chain.
  • Saccharides, structures, nutrition, digestion. Transport of glucose into cells. Glycolysis.
  • Metabolism of glycogen. Metabolism of fructose and galactose.
  • Surfactants (types, physiological roles). Lipids (structures).
  • Biological membranes. Membrane transport.
  • Lipids in nutrition, digestion. Catabolism of fatty acids, ketone bodies.
  • Desaturation of fatty acids. Cholesterol.
  • Metabolism of bile acids, eicosanoids, phospholipids.
  • Lipophilic vitamins, ROS. Lipid peroxidation.
  • Amino acids, proteins, structure, properties.
  • Hemoglobin, types, function, abnormal types.
  • Digestion of proteins, general features of amino acid metabolism, synthesis of urea.
  • Metabolism of amino acids I (catabolism, synthesis of non-essential AA, congenital disorders).
  • Metabolism of amino acids II (conversions to special products, heme).
  • Structure of bases, nucleosides, nucleotides, roles of nucleotides. Metabolism of purine and pyrimidine bases I.
  • Metabolism of purine and pyrimidine bases II.
  • DNA, RNA structure, replication, transcription.
  • Protein synthesis. Post-translation modification, collagen synthesis.
Literature
    required literature
  • Seminar texts available in Information system
  • RODWELL, Victor W., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY and P. Anthony WEIL. Harper's illustrated biochemistry. Thirty-first edition. New York: McGraw-Hill, 2018. x, 789. ISBN 9781260288421. info
    recommended literature
  • LIEBERMAN, Michael, Allan D. MARKS and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 4th ed. Baltimore: Lippincott, Williams & Wilkins, 2013. ix, 1014. ISBN 9781451100037. info
  • KOOLMAN, Jan and Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info
  • VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Textbook of biochemistry for medical students. Eighth edition. New Delhi: Jaypee The Health Sciences Publisher, 2016. xvi, 718. ISBN 9789385999741. info
Teaching methods
Seminar. The course is based on group discussion on the given topics. The outlines of discussion are in the recommended textbook. Complementary materials are available in section Study materials in IS MUNI.
Assessment methods
Course-unit credit. Conditions for giving the course-unit credit (both conditions should be met):
  • Full attendance in lessons. All absences should be justified and then made up.
  • At least 70% success rate from partial revision tests or 50% success rate in credit test, the resit credit test can be taken only once.
    The detailed conditions are posted in Information System (Study materials – Course-related instructions).
  • Language of instruction
    English
    Further comments (probably available only in Czech)
    The course is taught annually.
    The course is taught: every week.
    Information on the extent and intensity of the course: 60.
    Listed among pre-requisites of other courses
    The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021.
    • Enrolment Statistics (recent)
    • Permalink: https://is.muni.cz/course/med/autumn2022/aVLBC0321s