LF:MBKH081p Clinical hematology II - lectu - Course Information

MBKH081p Clinical hematology II - lectures

Extent and Intensity

3/0/0. 5 credit(s). Type of Completion: zk (examination).

Teacher(s)

RNDr. Ludmila Bourková (lecturer)
MUDr. Alena Buliková, Ph.D. (lecturer)
MUDr. Jan Kamelander (lecturer)
MUDr. Jarmila Kissová, Ph.D. (lecturer)
prof. MUDr. Miroslav Penka, CSc. (lecturer)
MUDr. Petr Smejkal, Ph.D. (lecturer)
RNDr. Jiřina Zavřelová (lecturer)
MUDr. Zdeňka Čermáková, Ph.D. (assistant)
doc. MUDr. Milan Dastych, CSc., MBA (assistant)
Michaela Gregorovičová (assistant)

Guaranteed by

prof. MUDr. Miroslav Penka, CSc.
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine

Timetable

Mon 19. 2. to Fri 6. 4. Thu 12:00–12:50 C05/107, Thu 13:00–13:50 C05/107, Thu 14:00–14:50 C05/107

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Bioanalyst - Specialist in Laboratory Methods (programme PřF, N-BCH)

Course objectives

The aim of the theoretical part of the course is to provide detailed information on methodologies utilizing the most recent knowledge of the art with respect to the present state of routine use of the current processes and technologies. Detailed knowledge of the indication and interpretation of laboratory diagnostic hematology methods in relation to clinical units and diagnoses.
This subject summarizes the information on the origin, development, structure and function of the hematopoietic cell and organ system as well as the system of blood clotting. Both are described both in health and in various innate and acquired diseases.
Upon completion of the course, the students will be informed in detail about the pathophysiological mechanisms, in the framework of clinical, but in particular about the laboratory findings of haematological diseases. They will be able to indicate, interpret and also introduce laboratory methods to diagnose, monitor and monitor the treatment of a wide range of diseases, both hematologically and in a condition where the involvement of any organ or system is reflected in a change in hematopoiesis and / or hemostasis. They will be able to interpret the results obtained during a laboratory haematological examination for the diagnosis or differential diagnosis. Special attention will be paid to the principles of laboratory diagnostics in hematology, instrumentation and laboratory diagnostics (reagents) during the course of study, including the principles of reliability detection and cost / benefit principles. Students will be introduced to the system of good laboratory practice, including internal and external quality control, follow-up processes, reproducibility and reliability testing and, last but not least, the principles of certification and accreditation at the hematological laboratory.

Learning outcomes

Upon completion of the course, the students will be informed in detail about the pathophysiological mechanisms, in the framework of clinical, but in particular about the laboratory findings of haematological diseases. They will be able to indicate, interpret and also introduce laboratory methods to diagnose, monitor and monitor the treatment of a wide range of diseases, both hematologically and in a condition where the involvement of any organ or system is reflected in a change in hematopoiesis and / or hemostasis. They will be able to interpret the results obtained during a laboratory haematological examination for the diagnosis or differential diagnosis. Special attention will be paid to the principles of laboratory diagnostics in hematology, instrumentation and laboratory diagnostics (reagents) during the course of study, including the principles of reliability detection and cost / benefit principles. Students will be introduced to the system of good laboratory practice, including internal and external quality control, follow-up processes, reproducibility and reliability testing and, last but not least, the principles of certification and accreditation at the hematological laboratory.

Syllabus

• Hematopoiesis in health and illness, principles of its management. Methods of detection of this process (tissue culture, detection of interleukins and growth factors, cytogenetics and molecular genetics to reveal innate processes).
• Red blood cell disorders:
• Differential diagnostics of anemias and principles of their detection: determination of parameters of blood count, reticulocytes, erythrocyte morphology, findings in bone marrow including necessary cytochemistry, hemoglobin and / or non-globulosine electrophoresis, isoelectric focusing, HLPC, flow cytometry for dif. anemic conditions, cytogenetic methods and molecular genetic genetics. dg. anemic syndromes.
• Polycythemia and secondary polyglobulia: basic laboratory parameters, indicative histopathological findings, diagnostics based on EEC growth, molecular diagnostics and differential diagnosis of inborn and acquired situations.
• Defects in the number and function of leukocytes: basic laboratory parameters, possibilities of laboratory diagnostics - blood picture morphology, flow cytometry, molecular diagnostic methods. Basic diff. cancer of the hematopoiesis.
• Platelet count and function disorders: basic laboratory methods, pseudotrombocytopenia and methods of detection; thrombocyte morphology, primary haemostasis (PFA) defects, detail - bleeding time and its limitation, platelet aggregation including modalities, flow cytometry, molecular diagnostics, HIT detection methods, TTC (ADAMST 13 + inhibitor).
• Diagnosis and differential diagnosis of haematological malignancies including myelodysplastic syndrome: WHO classification and its principles: Possible findings in blood stain, glass coating. Principles of diagnostics and differential diagnostics of peripheral and bone marrow coatings and their interpretation, limitations. Peripheral blood and bone marrow cytochemistry, benefits and limitations. Flow cytometry and its possibilities, advantages and limitations, cytogenetics and molecular genetics, possibilities and limitations.
• Investigation of Primary Haemostasis: Basic nosological units, congenital and acquired, frequency of occurrence. Platelet count, including modalities, their morphology. Functional examination of platelets including modalities, PFA, retraction, flow cytometry. Methods for diagnosis and differential diagnosis of von Willebrand's disease and von Willebrand's syndrome, dig. diagnosis and methods of therapy monitoring. Monitoring antiplatelet therapy.
• Investigation of Factors of Factors and Inhibitors: Basic nosological units (congenital and acquired), differential diagnostics. Methods for detecting activity / antigen of plasma factors or inhibitors. Differential diagnosis of congenital and acquired causes, resp. arteficial "laboratory deviations". Interpretation of treatment findings (heparin, LMWH, coumarins, pentasaccharides, xabans, dabigatran).
• Investigation of fibrinolysis processes: Basic and special examinations, their interpretation and possible findings in various clinical situations.
• Global trials for the assessment of blood clotting processes: Thrombin generation tests, detection of thrombin-antithrombin complexes, viscoelastic methods (TEG, ROTEM, free oscillatory rheometry). Their interpretation and contribution to clinical interpretation in specific situations. POCT, benefits and limitations.
• Good Laboratory Practice: Laboratory Manual, standard analytical and instrument operational procedures, internal and external quality control system, methodology of their nonconformities. Possible certification and accreditation in the Hematology Laboratory, recommendations of the CHS JEP laboratory section, their up-grade and publication. Communication of laboratory findings, legal aspects.
• Monitoring of antithrombotic medication. Basic tests and their influence on various antithrombotics. Specific tests and their interpretations. Possibilities to influence further routine or special haemocoagulation findings in antithrombotic medication.
• Information technologies: Their conditions, possibilities and limitations, connection to the hospital information system, possibilities of communication and potential problems, solution of non-conformities.

Literature

• Adam Z., Vorlíček J. a kol.: Hematologie II, Přehled maligních hematologických nemocí. Grada Publishing Praha, 2001, 21 – 677
• Penka M., Buliková A., Matýšková M., Zavřelová J.: Hematologie I,. Neonkologická hematologie. Grada Publishing Praha, 2001, 11 – 201
• Penka M.,Tesařová E. a kolektiv. Hematologie a transfuzní lékařství I, Hematologie. Grada, Praha, 2011: 5-421
• PECKA, Miroslav. Laboratorní hematologie v přehledu : fyziologie a patofyziologie krevní buňky. 1. vyd. Český Těšín: FINIDR, 2006, 304 s. ISBN 8086682021. info
• PECKA, Miroslav. Laboratorní hematologie v přehledu : fyziologie a patofyziologie hemostázy. 1. vyd. Český Těšín: FINIDR, 2004, 237 s. ISBN 808668203X. info

Teaching methods

lectures with use of audiovisual aids (ppt presentations, commented videos) includinginteractive inputs

Assessment methods

oral examination

Language of instruction

Czech

Further Comments

Study Materials

• Enrolment Statistics (Spring 2018, recent)
  
• Permalink: https://is.muni.cz/course/med/spring2018/MBKH081p