Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D., MBA (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
Mgr. Michal Vašina, Ph.D. (lecturer) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Microbiology (programme PřF, N-MIK)
- Course objectives
- The aim of the course is to provide comprehensive information on the molecular basis and major applications of recombinant DNA technology with an emphasis on environmentally friendly processes meeting modern trends of sustainable development.
The methodological part of the course provides a broad multidisciplinary overview of the fields of molecular biology, modern techniques of recombinant protein expression, synthetic biology, microfluidics and the use of artificial intelligence in the field.
The second part of the lectures focuses on the practical applications of recombinant DNA technology in the chemical and pharmaceutical industries, medicine, agriculture, and environmental protection.
Graduates will have a theoretical background and a comprehensive overview of the field relevant to their future employment in research laboratories, biomedical research, and development or the biotechnology industry. - Learning outcomes
- The student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA
- modern protein expression technologies
- basic methods in synthetic biology
- protein engineering
- microfluidics and "lab on chip" technologies
- artificial intelligence in life sciences
During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics
- long-term sustainable industries
- modern agriculture and biofuels
- environmental protection
At the end of this course, the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research, and development or biotechnology industry. - Syllabus
- Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- Advanced Molecular Biology Tools
- Protein Expression Technologies
- Introduction to Synthetic Biology
- Protein Engineering
- Microfludics - Lab on a Chip
- Artificial Intelligence in Life Sciences
- TECHNOLOGICAL LECTURES:
- Molecular Biotechnology Industry
- Molecular Biotechnology in Medicine I.
- Molecular Biotechnology in Medicine II.
- Molecular Biotechnology in Agriculture
- Environmental Molecular Biotechnology
- (the order of courses can be changed)
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ).
- Assessment methods
- Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain 10-12 questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F. - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9680en Artificial Intelligence in Biology, Chemistry, and Bioengineering
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Teacher's information
- https://loschmidt.chemi.muni.cz/peg/courses/
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2023
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D., MBA (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 16:00–17:50 B11/335
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Microbiology (programme PřF, N-MIK)
- Course objectives
- The aim of the course is to provide comprehensive information on the molecular basis and major applications of recombinant DNA technology with an emphasis on environmentally friendly processes meeting modern trends of sustainable development.
The methodological part of the course provides a broad multidisciplinary overview of the fields of molecular biology, modern techniques of recombinant protein expression, synthetic biology, microfluidics and the use of artificial intelligence in the field.
The second part of the lectures focuses on the practical applications of recombinant DNA technology in the chemical and pharmaceutical industries, medicine, agriculture, and environmental protection.
Graduates will have a theoretical background and a comprehensive overview of the field relevant to their future employment in research laboratories, biomedical research, and development or the biotechnology industry. - Learning outcomes
- The student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA
- modern protein expression technologies
- basic methods in synthetic biology
- protein engineering
- microfluidics and "lab on chip" technologies
- artificial intelligence in life sciences
During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics
- long-term sustainable industries
- modern agriculture and biofuels
- environmental protection
At the end of this course, the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research, and development or biotechnology industry. - Syllabus
- Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- Advanced Molecular Biology Tools
- Protein Expression Technologies
- Introduction to Synthetic Biology
- Protein Engineering
- Microfludics - Lab on a Chip
- Artificial Intelligence in Life Sciences
- TECHNOLOGICAL LECTURES:
- Molecular Biotechnology Industry
- Molecular Biotechnology in Medicine I.
- Molecular Biotechnology in Medicine II.
- Molecular Biotechnology in Agriculture
- Environmental Molecular Biotechnology
- (the order of courses can be changed)
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ).
- Assessment methods
- Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain 10-12 questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F. - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9680en Artificial Intelligence in Biology, Chemistry, and Bioengineering
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- https://loschmidt.chemi.muni.cz/peg/courses/
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2022
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D., MBA (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 16:00–17:50 B11/335
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Microbiology (programme PřF, N-MIK)
- Course objectives
- The aim of the course is to provide comprehensive information on the molecular basis and major applications of recombinant DNA technology with an emphasis on environmentally friendly processes meeting modern trends of sustainable development.
The methodological part of the course provides a broad multidisciplinary overview of the fields of molecular biology, modern techniques of recombinant protein expression, synthetic biology, microfluidics and the use of artificial intelligence in the field.
The second part of the lectures focuses on the practical applications of recombinant DNA technology in the chemical and pharmaceutical industries, medicine, agriculture, and environmental protection.
Graduates will have a theoretical background and a comprehensive overview of the field relevant to their future employment in research laboratories, biomedical research, and development or the biotechnology industry. - Learning outcomes
- The student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA
- modern protein expression technologies
- basic methods in synthetic biology
- protein engineering
- microfluidics and "lab on chip" technologies
- artificial intelligence in life sciences
During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics
- long-term sustainable industries
- modern agriculture and biofuels
- environmental protection
At the end of this course, the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research, and development or biotechnology industry. - Syllabus
- Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- Advanced Molecular Biology Tools
- Protein Expression Technologies
- Introduction to Synthetic Biology
- Protein Engineering
- Microfludics - Lab on a Chip
- Artificial Intelligence in Life Sciences
- TECHNOLOGICAL LECTURES:
- Molecular Biotechnology Industry
- Molecular Biotechnology in Medicine I.
- Molecular Biotechnology in Medicine II.
- Molecular Biotechnology in Agriculture
- Environmental Molecular Biotechnology
- (the order of courses can be changed)
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ).
- Assessment methods
- Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain 10-12 questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F. - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9680en Artificial Intelligence in Biology, Chemistry, and Bioengineering
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- https://loschmidt.chemi.muni.cz/peg/courses/
Bi7430 Molecular biotechnology
Faculty of Scienceautumn 2021
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D., MBA (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 11:00–12:50 B11/305
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Microbiology (programme PřF, N-MIK)
- Course objectives
- The aim of the course is to provide comprehensive information on the molecular basis and major applications of recombinant DNA technology with an emphasis on environmentally friendly processes meeting modern trends of sustainable development.
The methodological part of the course provides a broad multidisciplinary overview of the fields of molecular biology, modern techniques of recombinant protein expression, synthetic biology, microfluidics and the use of artificial intelligence in the field.
The second part of the lectures focuses on the practical applications of recombinant DNA technology in the chemical and pharmaceutical industries, medicine, agriculture, and environmental protection.
Graduates will have a theoretical background and a comprehensive overview of the field relevant to their future employment in research laboratories, biomedical research, and development or the biotechnology industry. - Learning outcomes
- The student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA
- modern protein expression technologies
- basic methods in synthetic biology
- protein engineering
- microfluidics and "lab on chip" technologies
- artificial intelligence in life sciences
During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics
- long-term sustainable industries
- modern agriculture and biofuels
- environmental protection
At the end of this course, the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research, and development or biotechnology industry. - Syllabus
- Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- Advanced Molecular Biology Tools
- Protein Expression Technologies
- Introduction to Synthetic Biology
- Protein Engineering
- Microfludics - Lab on a Chip
- Artificial Intelligence in Life Sciences
- TECHNOLOGICAL LECTURES:
- Molecular Biotechnology Industry
- Molecular Biotechnology in Medicine I.
- Molecular Biotechnology in Medicine II.
- Molecular Biotechnology in Agriculture
- Environmental Molecular Biotechnology
- (the order of courses can be changed)
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ).
- Assessment methods
- Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain 10-12 questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F. - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9680en Artificial Intelligence in Biology, Chemistry, and Bioengineering
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- https://loschmidt.chemi.muni.cz/peg/courses/
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2020
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D., MBA (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Ing. Andrea Smith, PhD. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 16:00–17:50 prace doma
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Microbiology (programme PřF, N-MIK)
- Course objectives
- The aim of the course is to provide comprehensive information on the molecular basis and major applications of recombinant DNA technology with an emphasis on environmentally friendly processes meeting modern trends of sustainable development.
The methodological part of the course provides a broad multidisciplinary overview of the fields of molecular biology, modern techniques of recombinant protein expression, synthetic biology, microfluidics and the use of artificial intelligence in the field.
The second part of the lectures focuses on the practical applications of recombinant DNA technology in the chemical and pharmaceutical industries, medicine, agriculture, and environmental protection.
Graduates will have a theoretical background and a comprehensive overview of the field relevant to their future employment in research laboratories, biomedical research, and development or the biotechnology industry. - Learning outcomes
- The student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA
- modern protein expression technologies
- basic methods in synthetic biology
- protein engineering
- microfluidics and "lab on chip" technologies
- artificial intelligence in life sciences
During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics
- long-term sustainable industries
- modern agriculture and biofuels
- environmental protection
At the end of this course, the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research, and development or biotechnology industry. - Syllabus
- Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- Basic Molecular Biology Tools
- Protein Expression Technologies
- Introduction to Synthetic Biology
- Protein Engineering
- Microfludics - Lab on a Chip
- Artificial Intelligence in Life Sciences
- TECHNOLOGICAL LECTURES:
- Molecular Biotechnology Industry
- Molecular Biotechnology in Medicine I.
- Molecular Biotechnology in Medicine II.
- Molecular Biotechnology in Agriculture
- Environmental Molecular Biotechnology
- (the order of courses can be changed)
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- Teaching will be organized online in the autumn 2020 in the MS Teams environment.
During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ). - Assessment methods
- Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain 10-12 questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F. - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9680en Artificial Intelligence in Biology, Chemistry, and Bioengineering
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- https://loschmidt.chemi.muni.cz/peg/courses/
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2019
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D., MBA (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Ing. Andrea Smith, PhD. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Thu 14:00–15:50 B11/335
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Microbiology (programme PřF, N-MIK)
- Course objectives
- The aim of the course is to provide comprehensive information on the molecular basis and major applications of recombinant DNA technology with an emphasis on environmentally friendly processes meeting modern trends of sustainable development.
The methodological part of the course provides a broad multidisciplinary overview of the fields of molecular biology, modern techniques of recombinant protein expression, synthetic biology, microfluidics and the use of artificial intelligence in the field.
The second part of the lectures focuses on the practical applications of recombinant DNA technology in the chemical and pharmaceutical industries, medicine, agriculture, and environmental protection.
Graduates will have a theoretical background and a comprehensive overview of the field relevant to their future employment in research laboratories, biomedical research, and development or the biotechnology industry. - Learning outcomes
- The student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA
- modern protein expression technologies
- basic methods in synthetic biology
- protein engineering
- microfluidics and "lab on chip" technologies
- artificial intelligence in life sciences
During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics
- long-term sustainable industries
- modern agriculture and biofuels
- environmental protection
At the end of this course, the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research, and development or biotechnology industry. - Syllabus
- Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- Basic Molecular Biology Tools
- Protein Expression Technologies
- Introduction to Synthetic Biology
- Protein Engineering
- Microfludics - Lab on a Chip
- Artificial Intelligence in Life Sciences
- TECHNOLOGICAL LECTURES:
- Molecular Biotechnology Industry
- Molecular Biotechnology in Medicine I.
- Molecular Biotechnology in Medicine II.
- Molecular Biotechnology in Agriculture
- Environmental Molecular Biotechnology
- (the order of courses can be changed)
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ).
- Assessment methods
- Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F. - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9680en Artificial Intelligence in Biology, Chemistry, and Bioengineering
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- https://loschmidt.chemi.muni.cz/peg/courses/
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2018
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. Andrea Schenkmayerová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 1. 10. to Fri 14. 12. Thu 10:00–11:50 B11/335
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.
- Learning outcomes
- Student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA;
- basic methods in synthetic biology;
- protein engineering;
- microfluidics and "lab on chip" technologies;
During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics;
- long-term sustainable industries;
- modern agriculture and biofuels;
- environmental protection;
At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry. - Syllabus
- Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- Basic Molecular Biology Tools
- Metabolic Engineering
- Protein Engineering
- Microfludics - Lab on a Chip
- TECHNOLOGICAL LECTURES:
- Biofuels
- Molecular Biotechnology Industry
- Environmental Molecular Biotechnology
- Molecular Biotechnology in Medicine I.
- Molecular Biotechnology in Medicine II.
- Molecular Biotechnology in Agriculture
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared.
- Assessment methods
- Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F). - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- https://loschmidt.chemi.muni.cz/peg/courses/
Bi7430 Molecular biotechnology
Faculty of Scienceautumn 2017
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Ing. Andrea Schenkmayerová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Lukáš Chrást (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 18. 9. to Fri 15. 12. Thu 16:00–17:50 D29/252-RCX1
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.
- Learning outcomes
- Student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA;
- basic methods in synthetic biology;
- protein engineering;
- microfluidics and "lab on chip" technologies;
During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics;
- long-term sustainable industries;
- modern agriculture and biofuels;
- environmental protection;
At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry. - Syllabus
- 1. Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- 2. Basic Molecular Biology Tools
- 3. Introduction to Synthetic Biology
- 4. Protein Engineering
- 5. Microfludics - Lab on a Chip
- TECHNOLOGICAL LECTURES:
- 6. Biofuels
- 7. Molecular Biotechnology Industry
- 8. Environmental Molecular Biotechnology
- 9. Molecular Biotechnology in Medicine I.
- 10. Molecular Biotechnology in Medicine II.
- 11. Molecular Biotechnology in Agriculture
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared.
- Assessment methods
- Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F). - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- https://loschmidt.chemi.muni.cz/peg/courses/
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2016
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. Táňa Koudeláková, Ph.D. (lecturer)
Mgr. Lukáš Chrást (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 19. 9. to Sun 18. 12. Thu 15:00–16:50 C05/114
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.
- Syllabus
- 1. Opening motivation lecture / Organization of the course
- 2. Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- 3. Molecular Biology Tools
- 4. Protein Engineering
- 5. Microfludics - Lab on a Chip
- TECHNOLOGICAL LECTURES:
- 6. Biofuels
- 7. Environmental molecular biotechnology
- 8. Molecular biotechnology industry
- 9. Molecular biotechnology in medicine I.
- 10. Molecular biotechnology in medicine II.
- 11. Molecular Biotechnology in Agriculture
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared.
- Assessment methods
- Short written tests will be prepared at the beginning of lectures No. 4, 6, 8 and 10. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F). - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2015
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Táňa Koudeláková, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Thu 12:00–13:50 B11/235
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.
- Syllabus
- 1. Opening motivation lecture / Organization of the course
- 2. Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- 3. Molecular Biology Tools
- 4. Protein Engineering
- 5. Metabolic Engineering I.
- 6. Metabolic Engineering II.
- 7. Microfludics - Lab on a Chip
- TECHNOLOGICAL LECTURES:
- 8. Molecular Biotechnology in Agriculture
- 9. Molecular biotechnology industry
- 10. Molecular biotechnology in medicine I.
- 11. Molecular biotechnology in medicine II.
- 12. Environmental molecular biotechnology
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared.
- Assessment methods
- Short written tests will be prepared at the beginning of lectures No. 4, 6, 8 and 10. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F). - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2014
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Koen Beerens, PhD. (lecturer)
Mgr. Martina Damborská (assistant) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 16:00–17:50 B11/306
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.
- Syllabus
- 1. Opening lecture / Organization of the course
- 2. Introduction to Molecular Biotechnology
- METHODOLOGICAL LECTURES:
- 3. Protein Engineering
- 4. Metabolic Engineering I.
- 5. Metabolic Engineering II.
- 6. Microfludics - Lab on a Chip
- TECHNOLOGICAL LECTURES:
- 7. Molecular Biotechnology in Agriculture
- 8. Molecular biotechnology industry
- 9. Molecular biotechnology in medicine I.
- 10. Molecular biotechnology in medicine II.
- 11. Molecular biotechnology in medicine III.
- 12. Environmental molecular biotechnology
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared.
- Assessment methods
- Short written tests will be prepared at the beginning of lectures No. 4, 6, 8 and 10. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F). - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2013
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (lecturer)
Koen Beerens, PhD. (lecturer)
Mgr. Martina Damborská (assistant) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 16. 9. to Fri 6. 12. Mon 9:00–10:50 B11/235
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.
- Syllabus
- 1. Opening lecture / Organization of the course
- 2. Introduction to Molecular Biotechnology
- 3. Protein Engineering
- 4. Metabolic Engineering I.
- 5. Metabolic Engineering II.
- 6. Molecular Biotechnology in Agriculture
- 7. Molecular biotechnology industry
- 8. Molecular biotechnology in medicine I.
- 9. Molecular biotechnology in medicine II.
- 10. Molecular biotechnology in medicine III.
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared.
- Assessment methods
- Short written tests will be prepared at the beginning of lectures No. 4, 6, 8 and 10. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.
The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F). - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8590 Protein Preparation and Characterization I - Expression and Purification
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer) - Guaranteed by
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Thu 10:00–11:50 B11/235
- Prerequisites
- Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- The course of molecular biotechnology is focused on the specific aspects of modern biotechnology covering the latest innovations and development of therapeutics, vaccines, biotransformations, transgenic plants and animals and the role of these modern technologies in sustainable living. The number of examples illustrates the latest principles and applications of recombinant DNA technology in up to date industrial, agricultural, pharmaceutical, and biomedical applications.
In this course, students learn about the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will acquire the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry. - Syllabus
- 1. Opening lecture / Organization of the course
- 2. Introduction to Molecular Biotechnology
- 3. Protein Engineering
- 4. Metabolic Engineering I.
- 5. Metabolic Engineering II.
- 6. Molecular biotechnology industry
- 7. Molecular biotechnology in medicine I.
- 8. Molecular biotechnology in medicine II.
- 9. Molecular Biotechnology in Agriculture
- 10. Molecular Biotechnology and Environmental Protection
- Literature
- An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
- GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
- Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info
- Teaching methods
- During lecturing, the powerpoint presentations are used, pdf materials for printing and notices are available, prepared are short written tests at the beginning of selected lectures and short exercises and discussions during lectures.
- Assessment methods
- Short written tests will be prepared at the beginning of lectures No. 4, 6, 8 and 10. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.
In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period. - Language of instruction
- Czech
- Follow-Up Courses
- Bi0210 Plant Gene Engineering
- Bi0951 The Basics of Bioethics for Biologists
- Bi5000 Bioinformatics
- Bi5220 Immunology
- Bi6384 Advanced and Applied Immunology
- Bi7410 Protein Engineering
- Bi8090 Gene engineering
- Bi8980 Protein Preparation and Characterization I - Expression and Purification
- Bi9060 Bioinformatics II – proteins
- Bi9080 Technical processes in biotechnology
- Bi9410 Structural Biology
- Bi9950 Introduction to bioethics
- C6210 Biotechnology
- C8857 Protein Preparation and Characterization III - Protein-mediated interactions
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2011
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 12:00–13:50 Bpt,01013
- Prerequisites
- The course is destined for students interested in practical use of recombinant DNA technology in molecular biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biology (Bi4020) and General Microbiology (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students should be able to explain principles of the use of recombinant DNA technology for gene manipulation and construction of genetically modified organisms producing commercial products. Using the acquired data they will be able to understand the different cloning systems used and their use in preparation of different commercial products. Based on the obtained knowledge, they will be able to assess and evaluate new genetically modified organisms.
- Syllabus
- Relationship between chemical production and biotechnologies.
- Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
- Recombinant DNA technology. Plasmid cloning vectors and their use.
- Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
- Vectors for cloning large fragments of DNA.
- Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
- Increasing of protein stability. Fusion proteins.
- Translation expression vectors. DNA integration into host chromosome.
- Heterologous protein production in eukaryotic cells.
- Directed mutagenesis and protein engineering.
- Microbial molecular biotechnology. Molecular diagnostics.
- Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
- Production of small biological molecules, antibiotics and biopolymers.
- Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
- Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.
- Literature
- ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
- GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info
- Teaching methods
- Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.
- Assessment methods
- The course is closed with a test and a spoken exam. The test contains 60 questions, which must be answered briefly. Each student who has correctly answered 40 questions is qualified for the spoken exam. The student answers 2 to 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2010
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 8:00–9:50 Bpt,01013
- Prerequisites
- The course is destined for students interested in practical use of recombinant DNA technology in molecular biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biology (Bi4020) and General Microbiology (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students should be able to explain principles of the use of recombinant DNA technology for gene manipulation and construction of genetically modified organisms producing commercial products. Using the acquired data they will be able to understand the different cloning systems used and their use in preparation of different commercial products. Based on the obtained knowledge, they will be able to assess and evaluate new genetically modified organisms.
- Syllabus
- Relationship between chemical production and biotechnologies.
- Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
- Recombinant DNA technology. Plasmid cloning vectors and their use.
- Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
- Vectors for cloning large fragments of DNA.
- Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
- Increasing of protein stability. Fusion proteins.
- Translation expression vectors. DNA integration into host chromosome.
- Heterologous protein production in eukaryotic cells.
- Directed mutagenesis and protein engineering.
- Microbial molecular biotechnology. Molecular diagnostics.
- Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
- Production of small biological molecules, antibiotics and biopolymers.
- Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
- Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.
- Literature
- ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
- GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info
- Teaching methods
- Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.
- Assessment methods
- The course is closed with a test and a spoken exam. The test contains 60 questions, which must be answered briefly. Each student who has correctly answered 40 questions is qualified for the spoken exam. The student answers 2 to 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2009
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 15:00–16:50 Bpt,01013
- Prerequisites
- The course is destined for students interested in practical use of recombinant DNA technology in molecular biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biology (Bi4020) and General Microbiology (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students should be able to explain principles of the use of recombinant DNA technology for gene manipulation and construction of genetically modified organisms producing commercial products. Using the acquired data they will be able to understand the different cloning systems used and their use in preparation of different commercial products. Based on the obtained knowledge, they will be able to assess and evaluate new genetically modified organisms.
- Syllabus
- Relationship between chemical production and biotechnologies.
- Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
- Recombinant DNA technology. Plasmid cloning vectors and their use.
- Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
- Vectors for cloning large fragments of DNA.
- Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
- Increasing of protein stability. Fusion proteins.
- Translation expression vectors. DNA integration into host chromosome.
- Heterologous protein production in eukaryotic cells.
- Directed mutagenesis and protein engineering.
- Microbial molecular biotechnology. Molecular diagnostics.
- Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
- Production of small biological molecules, antibiotics and biopolymers.
- Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
- Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.
- Literature
- ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
- GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info
- Teaching methods
- Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.
- Assessment methods
- The course is closed with a test and a spoken exam. The test contains 60 questions, which must be answered briefly. Each student who has correctly answered 40 questions is qualified for the spoken exam. The student answers 2 to 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 11:00–12:50 Bpt,01013
- Prerequisites
- The course is for the students with interest about molecular biotechnology. Prerequisites are exams from Molecular biology(Bi4020) and General microbiology(Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Definition of molecular biotechnology and its emergence. Impact of recombinant DNA technology on molecular biotechnology. Procedures enabling gene transfer from one organism into an other (vectors for cloning of DNA fragments of different lengths, construction and screening of gene and genome libraries, cDNA and metagenomic libraries). Manipulation of gene expression in prokaryotes (the use of strong and regulatable promotors, expression vectors, integration vectors, vectors for construction of fusion proteins). Heterologous protein production in eukaryotic cells. Microbial molecular biotechnology and its use in molecular diagnostics, in production of small molecules, therapeutic proteins and vaccines, in bioremediation and in biomass utilisation. The next use of transgenic microorganisms, plants and animals. Regulating of GMO use. This course is supplemented by practical course Bi7430c.
At the end of this course, student should be able to explain the use of recombinant DNA technology for the construction of genetic modified organisms producing commercial products. Using aquired data he will be able to understand new genetic modified organisms. - Syllabus
- Relationship between chemical production and biotechnologies.
- Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
- Recombinant DNA technology. Plasmid cloning vectors and their use.
- Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
- Vectors for cloning large fragments of DNA.
- Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
- Increasing of protein stability. Fusion proteins.
- Translation expression vectors. DNA integration into host chromosome.
- Heterologous protein production in eukaryotic cells.
- Directed mutagenesis and protein engineering.
- Microbial molecular biotechnology. Molecular diagnostics.
- Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
- Production of small biological molecules, antibiotics and biopolymers.
- Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
- Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.
- Literature
- ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
- GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info
- Assessment methods
- Lectures are enclosed by test and oral exam.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2007
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 9:00–10:50 Bpt,01013
- Prerequisites
- Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creating and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Directed mutagenesis and protein engineering. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
- Syllabus
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
- Literature
- Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.
- Assessment methods (in Czech)
- Předmět je ukončen zkouškou
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2006
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 14:00–15:50 Bpt,01013
- Prerequisites
- Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creating and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Directed mutagenesis and protein engineering. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
- Syllabus
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
- Literature
- Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.
- Assessment methods (in Czech)
- Předmět je ukončen zkouškou
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2005
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 13:00–14:50 Bpt,01013
- Prerequisites
- Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creating and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Directed mutagenesis and protein engineering. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
- Syllabus
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
- Literature
- Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.
- Assessment methods (in Czech)
- Předmět je ukončen zkouškou
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2004
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 11:00–12:50 Bpt,01013
- Prerequisites
- Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creating and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Directed mutagenesis and protein engineering. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
- Syllabus
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creation and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Direct mutagenesis and protein engineering. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
- Literature
- 1. Watson J.D., Gilman M., Witkowski J. Zoller M. Recombinant DNA (2nd ed.), SAB, New York 1992. 2. Glick B.R., Pasternak J.J. Molecular Biotechnology, ASM Press, Washington 1994.
- Assessment methods (in Czech)
- Předmět je ukončen zkouškou
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2003
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creating and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Directed mutagenesis and protein engineering. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
- Syllabus
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creation and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Direct mutagenesis and protein engineering. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
- Literature
- 1. Watson J.D., Gilman M., Witkowski J. Zoller M. Recombinant DNA (2nd ed.), SAB, New York 1992. 2. Glick B.R., Pasternak J.J. Molecular Biotechnology, ASM Press, Washington 1994.
- Assessment methods (in Czech)
- Předmět je ukončen zkouškou
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7430 Úvod do molekulární biotechnologie
Faculty of ScienceAutumn 2002
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creating and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Directed mutagenesis and protein engineering. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
- Syllabus
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creation and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Direct mutagenesis and protein engineering. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
- Literature
- 1. Watson J.D., Gilman M., Witkowski J. Zoller M. Recombinant DNA (2nd ed.), SAB, New York 1992. 2. Glick B.R., Pasternak J.J. Molecular Biotechnology, ASM Press, Washington 1994.
- Assessment methods (in Czech)
- Předmět je ukončen zkouškou
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2011 - acreditation
The information about the term Autumn 2011 - acreditation is not made public
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in practical use of recombinant DNA technology in molecular biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biology (Bi4020) and General Microbiology (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students should be able to explain principles of the use of recombinant DNA technology for gene manipulation and construction of genetically modified organisms producing commercial products. Using the acquired data they will be able to understand the different cloning systems used and their use in preparation of different commercial products. Based on the obtained knowledge, they will be able to assess and evaluate new genetically modified organisms.
- Syllabus
- Relationship between chemical production and biotechnologies.
- Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
- Recombinant DNA technology. Plasmid cloning vectors and their use.
- Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
- Vectors for cloning large fragments of DNA.
- Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
- Increasing of protein stability. Fusion proteins.
- Translation expression vectors. DNA integration into host chromosome.
- Heterologous protein production in eukaryotic cells.
- Directed mutagenesis and protein engineering.
- Microbial molecular biotechnology. Molecular diagnostics.
- Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
- Production of small biological molecules, antibiotics and biopolymers.
- Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
- Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.
- Literature
- ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
- GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info
- Teaching methods
- Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.
- Assessment methods
- The course is closed with a test and a spoken exam. The test contains 60 questions, which must be answered briefly. Each student who has correctly answered 40 questions is qualified for the spoken exam. The student answers 2 to 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in practical use of recombinant DNA technology in molecular biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biology (Bi4020) and General Microbiology (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students should be able to explain principles of the use of recombinant DNA technology for gene manipulation and construction of genetically modified organisms producing commercial products. Using the acquired data they will be able to understand the different cloning systems used and their use in preparation of different commercial products. Based on the obtained knowledge, they will be able to assess and evaluate new genetically modified organisms.
- Syllabus
- Relationship between chemical production and biotechnologies.
- Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
- Recombinant DNA technology. Plasmid cloning vectors and their use.
- Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
- Vectors for cloning large fragments of DNA.
- Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
- Increasing of protein stability. Fusion proteins.
- Translation expression vectors. DNA integration into host chromosome.
- Heterologous protein production in eukaryotic cells.
- Directed mutagenesis and protein engineering.
- Microbial molecular biotechnology. Molecular diagnostics.
- Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
- Production of small biological molecules, antibiotics and biopolymers.
- Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
- Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.
- Literature
- ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
- GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info
- Teaching methods
- Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.
- Assessment methods
- The course is closed with a test and a spoken exam. The test contains 60 questions, which must be answered briefly. Each student who has correctly answered 40 questions is qualified for the spoken exam. The student answers 2 to 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creating and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Directed mutagenesis and protein engineering. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
- Syllabus
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
- Literature
- Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.
- Assessment methods (in Czech)
- Předmět je ukončen zkouškou
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Enrolment Statistics (recent)